sj-pdf-1-imr-10.1177_03000605211058868 - Supplemental material for Management of inferior vena cava injury and secondary thrombosis after percutaneous nephrolithotomy: a case report

Supplemental material, sj-pdf-1-imr-10.1177_03000605211058868 for Management of inferior vena cava injury and secondary thrombosis after percutaneous nephrolithotomy: a case report by Pan Li, Dengjiu Mao, Jie Zhou and Hongmei Sun in Journal of International Medical Research</p

Template-Free Synthesis of Renewable Macroporous Carbon via Yeast Cells for High-Performance Supercapacitor Electrode Materials

The urgent need for sustainable development has forced material scientists to explore novel materials for next-generation energy storage devices through a green and facile strategy. In this context, yeast, which is a large group of single cell fungi widely distributed in nature environments, will be an ideal candidate for developing effective electrode materials with fascinating structures for high-performance supercapacitors. With this in mind, herein, we present the first example of creating three-dimensional (3D) interpenetrating macroporous carbon materials via a template-free method, using the green, renewable, and widespread yeast cells as the precursors. Remarkably, when the as-prepared materials are used as the electrode materials for supercapacitors, they exhibit outstanding performance with high specific capacitance of 330 F g^–1 at a current density of 1 A g^–1, and good stability, even after 1000 charge/discharge cycles. The approach developed in this work provides a new view of making full use of sustainable resources endowed by nature, opening the avenue to designing and producing robust materials with great promising applications in high-performance energy-storage devices

Synthesis of Cationic N-Heterocyclic Carbene Lanthanide Bromide and the Influence of N-Heterocyclic Carbene and Lanthanide Metals

The lithium salt of the novel amido-phenoxo-functionalized NHC L (L = [3,5-tBu2-2-(O)C6H2CH(nBu)NH(CH2)2(C{N(CH)2NiPr})]), (LiL)2 (1), was synthesized by the direct reaction of the salicylaldimino-functionalized imidazolium salt H2L′Br (L′ = [3,5-tBu2-2-(O)C6H2CHN(CH2)2(C{N(CH)2NiPr})]) with 2 equiv of LinBu and structurally characterized. Treatment of LnCl3 with in situ formed 1 in a molar ratio of 1:1 at room temperature yielded the first cationic complexes [LnL2]+[Br]− (Ln = Y (2), Lu (3), Er (4)) in good yields. Treatment of Li(THF)Ln(NiPr2)4 with 2 equiv of H2L′Br at −78 °C afforded the cationic complexes [L′2Ln(THF)]+[Br]− (Ln = Y (5) and Yb (6)) in desired yields. Each central metal in cations [LnL2]+ for 2−4 is coordinated by two L ligands, while the central metal in [L′2Ln(THF)]+ for 5 and 6 is ligated by two ligands and one THF molecule. The structures of NHC lanthanide bromides have been found to depend on both the size of the metal and the ligand

Lanthanide Carbene Halides through Protonolysis of Ln−N Bonds by Imidazolium Salts: Synthesis and Structure of Salicylaldiminato-Functionalized N-Heterocyclic Carbene Lanthanide Bromides

The three salicylaldiminato-functionalized N-heterocyclic carbene lanthanide bromides L2LnBr (Ln = Nd (2), Sm (3), Er (4); L = [3,5-tBu2-2-(O)C6H2CHNCH2CH2(C{NCHCHNiPr})]) with early to late lanthanide metals were conveniently synthesized in moderate yields by a protonolysis strategy via the direct reaction of LiLn(NiPr2)4 with [H2L]Br (1). All complexes were characterized by X-ray crystal determinations

Relationship between Chemical Structure and Supramolecular Effective Molarity for Formation of Intramolecular H‑Bonds

Effective molarity (EM) is a key parameter that determines the efficiency of a range of supramolecular phenomena from the folding of macromolecules to multivalent ligand binding. Coordination complexes formed between zinc porphyrins equipped H-bond donor sites and pyridine ligands equipped with H-bond acceptor sites have allowed systematic quantification of EM values for the formation of intramolecular H-bonds in 240 different systems. The results provide insights into the relationship of EM to supramolecular architecture, H-bond strength, and solvent. Previous studies on ligands equipped with phosphonate diester and ether H-bond acceptors were inconclusive, but the experiments described here on ligands equipped with phosphine oxide, amide, and ester H-bond acceptors resolve these ambiguities. Chemical double-mutant cycles were used to dissect the thermodynamic contributions of individual H-bond interactions to the overall stabilities of the complexes and hence determine the values of EM, which fall in the range 1–1000 mM. Solvent has little effect on EM, and the values measured in toluene and 1,1,2,2-tetrachloroethane are similar. For H-bond acceptors that have similar geometries but different H-bond strengths (amide and ester), the values of EM are very similar. For H-bond acceptors that have different geometries but similar H-bond strengths (amide and phosphonate diester), there is little correlation between the values of EM. These results imply that supramolecular EMs are independent of solvent and intrinsic H-bond strength but depend on supramolecular architecture and geometric complementarity

Nickel-Catalyzed Reductive Cross-Coupling of Benzyl Chlorides with Aryl Chlorides/Fluorides: A One-Pot Synthesis of Diarylmethanes

The first nickel-catalyzed, magnesium-mediated reductive cross-coupling between benzyl chlorides and aryl chlorides or fluorides is reported. A variety of diarylmethanes can be prepared in good to excellent yields in a one-pot manner using easy-to-access mixed PPh₃/NHC Ni­(II) complexes of Ni­(PPh₃)­(NHC)­Br₂ (NHC = 1,3-bis­(2,6-diisopropylphenyl)­imidazol-2-ylidene, IPr, <b>1a</b>; 1,3-di-<i>tert-</i>butylimidazol-2-ylidene, ItBu, <b>1b</b>) as catalyst precursors. Activation of polychloroarenes or chemoselective cross-coupling based on the difference in catalytic activity between <b>1a</b> and <b>1b</b> is used to construct <i>oligo</i>-diarylmethane motifs

DataSheet_1_DCE-MRI and DWI can differentiate benign from malignant prostate tumors when serum PSA is ≥10 ng/ml.zip

BackgroundThis study investigated the diagnostic utility of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and diffusion-weighted imaging (DWI) parameters for distinguishing between benign and malignant prostate tumors when serum prostate-specific antigen (PSA) level is ≥10 ng/ml.MethodsPatients with prostate cancer (PCa) and benign prostatic hyperplasia (BPH) with serum PSA ≥10 ng/ml before treatment were recruited. Transrectal ultrasound-guided biopsy or surgery was performed for tumor classification and patients were stratified accordingly into PCa and BPH groups. Patients underwent DCE-MRI and DWI scanning and the transfer constant (Ktrans), rate constant (Kep), fractional volume of the extravascular extracellular space, plasma volume (Vp), and Prostate Imaging Reporting and Data System Version 2 (PI-RADS v2) score were determined. The apparent diffusion coefficient (ADC) was calculated from DWI. The diagnostic performance of these parameters was assessed by receiver operating characteristic (ROC) curve analysis, and those showing a significant difference between the PCa and BPH groups were combined into a multivariate logistic regression model for PCa diagnosis. Spearman’s correlation was used to analyze the relationship between Gleason score and imaging parameters.ResultsThe study enrolled 65 patients including 32 with PCa and 33 with BPH. Ktrans (P=0.006), Kep (P=0.001), and Vp (P=0.009) from DCE-MRI and ADC (PConclusionDCE-MRI and DWI parameters can distinguish between benign and malignant prostate tumors in patients with serum PSA ≥10 ng/ml.</p

Effects of RNAi on Cbfa1 gene expression in antler stem cell-derived nodules.

<p>A: qPCRof Cbfa1. Note that five of six siRNA target sequences (S1, S3, S4, S5, S6)down-regulated Cbfa1 gene expression compared to the scrambled sequence (C), particularly the S6 sequence that had most significant effects and knocked down expression of Cbfa1 gene up to 88%. B–D: Immunohistochemistry of Cbfa1 (400X). B, Section from the uninfected nodule. C, Section from the S6-infected nodule. D, Section from the S6-infected nodule but in the absence of the primary antibody. Note that uninfected nodule tissue had the strongest staining, and the staining of the S6-infected nodule tissue was significantly weaker, but still stronger than the S6-infected nodule in the absence of the primary antibody. E: Western blot analysis of Cbfa1 gene expression. 1 and 2, Cbfa1 protein bands from the two uninfected nodules; 3 and 4, from two of the S6-infected nodules. Note that the band sizes from the uninfected nodules were substantially bigger than those from the S6-infected nodules; whereas, no difference in GAPDH (control) could be visualised amongst the bands between the infected and uninfected nodules.</p

Image_3_MaCDSP32 From Mulberry Enhances Resilience Post-drought by Regulating Antioxidant Activity and the Osmotic Content in Transgenic Tobacco.TIF

Desiccation tolerance is a complex phenomenon that depends on the regulated expression of numerous genes during dehydration and subsequent rehydration. Our previous study identified a chloroplast drought-induced stress protein (MaCDSP32) in mulberry, a thioredoxin (Trx) that is upregulated under drought conditions and is likely to confer drought tolerance to transgenic plants. Mulberry (Morus spp.) is an ecologically and economically important perennial woody plant that is widely used in forest management to combat desertification. However, its stress tolerance physiology is not well understood. In this study, the functions of MaCDSP32 gene were investigated. The expression of MaCDSP32 exhibited a circadian rhythm and was induced by mild and severe water deficits. Under abiotic stress, MaCDSP32-overexpressing plants exhibited increased stress sensitivity with lower water retention capacity and more severe lipid peroxidation than the wild-type (WT) plants. Furthermore, the activity of superoxide dismutase (SOD), the contents of proline and soluble sugars and the expression of stress-related transcription factors were lower in the MaCDSP32-overexpressing plants than in the WT plants. However, the MaCDSP32-overexpressing lines exhibited stronger recovery capability after rewatering post-drought. Moreover, the SOD enzyme activity, proline content, and soluble sugar content were higher in the transgenic plants after rewatering than in the WT plants. The production of the reactive oxygen species (ROS) H2O2 and O2– was significantly lower in the transgenic plants than in the WT plants. In addition, under abiotic stress, the MaCDSP32-overexpressing lines exhibited improved seed germination and seedling growth, these effects were regulated by a positive redox reaction involving MaCDSP32 and one of its targets. In summary, this study indicated that MaCDSP32 from mulberry regulates plant drought tolerance and ROS homeostasis mainly by controlling SOD enzyme activity and proline and soluble sugar concentrations and that this control might trigger the stress response during seed germination and plant growth. Overall, MaCDSP32 exerts pleiotropic effects on the stress response and stress recovery in plants.</p

DataSheet_2_DCE-MRI and DWI can differentiate benign from malignant prostate tumors when serum PSA is ≥10 ng/ml.zip

BackgroundThis study investigated the diagnostic utility of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and diffusion-weighted imaging (DWI) parameters for distinguishing between benign and malignant prostate tumors when serum prostate-specific antigen (PSA) level is ≥10 ng/ml.MethodsPatients with prostate cancer (PCa) and benign prostatic hyperplasia (BPH) with serum PSA ≥10 ng/ml before treatment were recruited. Transrectal ultrasound-guided biopsy or surgery was performed for tumor classification and patients were stratified accordingly into PCa and BPH groups. Patients underwent DCE-MRI and DWI scanning and the transfer constant (Ktrans), rate constant (Kep), fractional volume of the extravascular extracellular space, plasma volume (Vp), and Prostate Imaging Reporting and Data System Version 2 (PI-RADS v2) score were determined. The apparent diffusion coefficient (ADC) was calculated from DWI. The diagnostic performance of these parameters was assessed by receiver operating characteristic (ROC) curve analysis, and those showing a significant difference between the PCa and BPH groups were combined into a multivariate logistic regression model for PCa diagnosis. Spearman’s correlation was used to analyze the relationship between Gleason score and imaging parameters.ResultsThe study enrolled 65 patients including 32 with PCa and 33 with BPH. Ktrans (P=0.006), Kep (P=0.001), and Vp (P=0.009) from DCE-MRI and ADC (PConclusionDCE-MRI and DWI parameters can distinguish between benign and malignant prostate tumors in patients with serum PSA ≥10 ng/ml.</p