MOESM1 of CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis

Additional file 1. Supplementary materials and methods. Subcellular fraction assay. The separation of cytoplasmic and nuclear fractions was performed using Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Wilmington, DE, USA) following the manufacturer’s protocols. Total RNA extracted from each fraction was used for detection of circNFIX and miR-378e by qRT-PCR assay. The relative expression levels of circNFIX and miR-378e in cytoplasmic and nuclear fractions were analyzed using GAPDH or U6 as cytoplasmic or nuclear control, respectively

MOESM2 of CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis

Additional file 2: Figure S1. CircNFIX was more resistant to RNase R than NFIX in glioma cells. (A) NFIX was a host gene of circNFIX (hsa_circ_0049658) and sanger sequencing validated the sequence on the junction sites of circNFIX. (B and C) The expression levels of circNFIX and NFIX were measured in U251 and T98 cells after treatment of RNase R. ***P<0.001. Figure S2. CircNFIX and miR-378e were predominantly localized in the cytoplasm. (A-D) The expression levels of circNFIX and miR-378e were measured in cytoplasmic and nuclear fractions, with GAPDH and U6 as the internal controls, respectively. Figure S3. RPN2 promoted the activation of ERK pathway in glioma cells. (A and B) The protein levels of p-ERK, ERK, p=MEK and MEK were measured in U251 cells transfected with pcDNA or RPN2 and T98 cells transfected with si-NC or si-RPN2. ***P<0.001

Fabrication of Mesoporous Co₃O₄ from LP-FDU-12 via Nanocasting Route and Effect of Wall/Pore Size on Their Magnetic Properties

Highly ordered mesoporous Co₃O₄ nanostructures were prepared using LP-FDU-12 as hard templates. By changing the hydrothermal temperature or by the acid treatment of the LP-FDU-12 template, Co₃O₄ replicas with different cell parameters and wall thicknesses have been obtained. The structure and textural characteristics of both LP-FDU-12 and Co₃O₄ replicas were investigated by X-ray diffraction, transmission electron microscopy, and N₂ adsorption–desorption isotherm analysis. The cell parameter and wall thickness of a mesoporous Co₃O₄ have been varied systematically within the ranges 30.4–33.9 and 24.8–18.2 nm, respectively, and the materials exhibit surface areas in the 29.6–52.9 m² g^–1 range, while preserving a highly ordered 3D pore structure and highly crystalline walls. Most importantly, magnetic studies show that the factors which affect the magnetic behavior in the Co₃O₄ nanosphere system are not only the sphere size but also the space-filled parameter at the nanoscale

Very Large, Soluble Endohedral Fullerenes in the Series La₂C₉₀ to La₂C₁₃₈: Isolation and Crystallographic Characterization of La₂@<i>D</i>₅(450)-C₁₀₀

An extensive series of soluble dilanthanum endohedral fullerenes that extends from La2C90 to La2C138 has been discovered. The most abundant of these, the nanotubular La2@D5(450)-C100, has been isolated in pure form and characterized by single-crystal X-ray diffraction

Very Large, Soluble Endohedral Fullerenes in the Series La₂C₉₀ to La₂C₁₃₈: Isolation and Crystallographic Characterization of La₂@<i>D</i>₅(450)-C₁₀₀

An extensive series of soluble dilanthanum endohedral fullerenes that extends from La2C90 to La2C138 has been discovered. The most abundant of these, the nanotubular La2@D5(450)-C100, has been isolated in pure form and characterized by single-crystal X-ray diffraction