Image_1_Extracellular Vesicles Long Non-Coding RNA AGAP2-AS1 Contributes to Cervical Cancer Cell Proliferation Through Regulating the miR-3064-5p/SIRT1 Axis.tif

Cervical cancer is one of the most severe and prevalent female malignancies and a global health issue. The molecular mechanisms underlying cervical cancer development are poorly investigated. As a type of extracellular membrane vesicles, EVs from cancer cells are involved in cancer progression by delivering regulatory factors, such as proteins, microRNAs (miRNAs), and long non-coding RNAs (lncRNAs). In this study, we identified an innovative function of extracellular vesicle (EV) lncRNA AGAP2-AS1 in regulating cervical cancer cell proliferation. The EVs were isolated from the cervical cancer cells and were observed by transmission electron microscopy (TEM) and were confirmed by analyzing exosome markers. The depletion of AGAP2-AS1 by siRNA significantly reduced its expression in the exosomes from cervical cancer and in the cervical cancer treated with AGAP2-AS1-knockdown exosomes. The expression of AGAP2-AS1 was elevated in the clinical cervical cancer tissues compared with the adjacent normal tissues. The depletion of EV AGAP2-AS1 reduced cell viabilities and Edu-positive cervical cancer cells, while it enhanced cervical cancer cell apoptosis. Tumorigenicity analysis in nude mice showed that the silencing of EV AGAP2-AS1 attenuated cervical cancer cell growth in vivo. Regarding the mechanism, we identified that AGAP2-AS1 increased SIRT1 expression by sponging miR-3064-5p in cervical cancer cells. The overexpression of SIRT1 or the inhibition of miR-3064-5p reversed EV AGAP2-AS1 depletion-inhibited cancer cell proliferation in vitro. Consequently, we concluded that EV lncRNA AGAP2-AS1 contributed to cervical cancer cell proliferation through regulating the miR-3064-5p/SIRT1 axis. The clinical values of EV lncRNA AGAP2-AS1 and miR-3064-5p deserve to be explored in cervical cancer diagnosis and treatments.</p

Synthesis of a Novel Quinoline Skeleton Introduced Cationic Polyfluorene Derivative for Multimodal Antimicrobial Application

A new functional polyfluorene derivative containing quinoline skeleton and quarternary ammonium group (QAG) modified side chains (PFPQ) was synthesized and characterized. The multimodal antimicrobial effect toward Gram-negative E. coli was achieved by the dark toxicity resulting from the quinoline skeleton, QAG, and light toxicity resulting from reactive oxygen species (ROS) produced by the main backbone of PFPQ under white light. The mechanism of interaction between PFPQ and bacteria was also demonstrated. PFPQ bound to E. coli mainly through electrostatic interactions causing nearly 50% bacterial death in the absence of light irradiation, and the huge capability of PFPQ to generate ROS under white light opened another bactericidal mode. The killing efficiency was more than 99% upon relatively mild irradiation under white light (400–800 nm) with a light dose of 18 J·cm^–2. PFPQ with the incorporation of quinoline into the backbones will provide a new versatile strategy to achieve the multimodal antimicrobial effect to fight against resistant bacteria

Enantioselective Conjugate Addition of 2‑Acetyl Azaarenes to β,β‑Disubstituted Nitroalkene for the Construction of All-Carbon Quaternary Stereocenters

The first highly enantioselective conjugate addition of 2-acetyl azaarenes to α-substituted-β-nitro­acrylates was successfully realized under mild conditions by a Ni­(II)-bisoxa­zoline complex, providing the desired adducts bearing an all-carbon quaternary stereocenter in high yield with excellent enantioselectivity. The products obtained in this system could be readily converted into optically active β^2,2-amino esters, succinates, lactones, and lactams

Phylogenic tree of the HIV-1 sequences.

<p>Phylogenic tree of the HIV-1 sequences.</p

Characteristics of donors with drug resistance of potential low-level resistance or above (N = 27).

<p>Characteristics of donors with drug resistance of potential low-level resistance or above (N = 27).</p

Demographic characteristics and subtypes of 205 infected donors whose HIV genotypes and DRMs were successfully analyzed.

<p>Demographic characteristics and subtypes of 205 infected donors whose HIV genotypes and DRMs were successfully analyzed.</p

Summary of HIV testing and DRMs analysis among blood donors from five Chinese blood centers, April 2012 to June 2014.

<p>Summary of HIV testing and DRMs analysis among blood donors from five Chinese blood centers, April 2012 to June 2014.</p

Infection stages of 259 HIV infected donors from five Chinese blood centers.

<p>(N, %)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179328#t001fn001" target="_blank">^a</a>.</p

Three Serologically positive but viral load not detected samples^a.

<p>Three Serologically positive but viral load not detected samples<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179328#t004fn001" target="_blank">^a</a>.</p