Additional file 1 of Genome-wide DNA N6-methyladenosine in Aeromonas veronii and Helicobacter pylori

Additional file 1. The commands for processing SMRT sequencing data

DataSheet_1_Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens.pdf

Clostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens. Since the use of antibiotics in feed is withdrawn, it is imperative to find out suitable alternatives to control NE. Baicalin-aluminum complex is synthesized from baicalin, a flavonoid isolated from Scutellaria baicalensis Georgi. The present study investigated the effects of baicalin-aluminum on the virulence-associated traits and virulence genes expression of C. perfringens CVCC2030, it also evaluated the in vivo therapeutic effect on NE. The results showed that baicalin-aluminum inhibited bacterial hemolytic activity, diminished biofilm formation, attenuated cytotoxicity to Caco-2 cells, downregulated the expression of genes encoding for clostridial toxins and extracellular enzymes such as alpha toxin (CPA), perfringolysin O (PFO), collagenase (ColA), and sialidases (NanI, NanJ). Additionally, baicalin-aluminum was found to negatively regulate the expression of genes involved in quorum sensing (QS) communication, including genes of Agr QS system (agrB, agrD) and genes of VirS/R two-component regulatory system (virS, virR). In vivo experiments, baicalin-aluminum lightened the intestinal lesions and histological damage, it inhibited pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) expression in the jejunal and ileal tissues. Besides, baicalin-aluminum alleviated the upregulation of C. perfringens and Escherichia coli and raised the relative abundance of Lactobacillus in the ileal digesta. This study suggests that baicalin-aluminum may be a potential candidate against C. perfringens infection by inhibiting the virulence-associated traits and virulence genes expression.</p

High-Temperature and Salt-Resistant Self-Suspending Proppant for Hydraulic Fracturing

In recent years, new technologies involving self-suspending proppant (SSP), which improves proppant distribution, reduces the use of chemicals in fracturing fluids, and simplifies the fracturing construction process, have become a focus of hydraulic fracturing research. However, the available products have poor suspension stability, cannot withstand high temperature and salt environments, and are highly damaging to reservoirs. In this study, we presented a novel self-suspending (HTS-SSP) proppant with excellent suspension stability, temperature resistance, and salt resistance, prepared from quartz sand, silane coupling agent, and modified polyacrylamide to achieve these properties. Wettability tests, surface morphology analysis, and Fourier transform infrared spectroscopy analysis proved that 3-methacryloxypropyltrmethoxysilane (KH570) successfully modifies quartz sand, and the HTS-SSP was synthesized. In terms of the suspension performance, HTS-SSP exhibited exceptional suspension stability due to the incorporation of KH570. It remained suspended at 150 °C or 24 wt % brine and showed excellent temperature and salt resistance. The gel-breaking fluid of the surface polymer of HTS-SSP had low damage to the reservoir, much less than the guar gum fluid. The difference between the conductivity of the aggregate remaining after HTS-SSP gel-breaking and that of unmodified quartz sand was not significant. The synthesized HTS-SSP demonstrates outstanding comprehensive performance and is an ideal candidate for reservoir development under harsh temperature and salinity conditions