29 research outputs found

    Biological activity of reporter proteins in physiological buffer or TRIzol lysates.

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    <p>Hek293 cells expressing either GFP or Luc were directly lysed in physiological Glo Lysis buffer (GLB) or TRIzol, and GFP or Luc activity were measured. Reporter proteins were verified by western blotting.</p

    Solubility of denatured and nucleic acid-free total cell proteins in pure water.

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    <p>*Initial protein concentrations were adjusted to 1 mg/mL before dialysis.</p><p>Solubility of denatured and nucleic acid-free total cell proteins in pure water.</p
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