Additional file 1 of Long-term renal graft outcome after parathyroidectomy - a retrospective single centre study

Additional file 1. Correlation of the GFR-change with clinical factors. The table displays the variables that were not significant in univariate analyses and not considered in the different multivariable models

Effect of SGLT2is on AKI in observational cohort studies.

AKI, acute kidney injury; CI, confidence interval; SGLT2i, sodium-glucose cotransporter-2 inhibitor.</p

Effect of SGLT2is on serious and nonserious AKI AEs in RCTs (Ease 2 and 3 are two separate trials).

AE, adverse event; AKI, acute kidney injury; CI, confidence interval; RCT, randomized controlled trial; SAE, serious AE; SGLT2i, sodium-glucose cotransporter-2 inhibitor.</p

PRISMA diagram.

AE, adverse event; AKI, acute kidney injury; NCBI, National Center for Biotechnology Information; PRISMA, Preferred Reporting Items for Systematic Reviews and Meta-Analyses; SAE, serious AE.</p

Effectiveness of a Fixed-Dose, Single-Pill Combination of Perindopril and Amlodipine in Patients with Hypertension: A Non-Interventional Study

Article full textThe full text of this article can be found here. https://link.springer.com/article/10.1007/s12325-018-0675-3Provide enhanced content for this articleIf you are an author of this publication and would like to provide additional enhanced content for your article then please contact [email protected] journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content.Other enhanced features include, but are not limited to:• Slide decks• Videos and animations• Audio abstracts • Audio slides </p

Effect of SGLT2is on serious AKI AEs in RCTs (Ease 2 and 3 are two separate trials).

AE, adverse event; AKI, acute kidney injury; CI, confidence interval; RCT, randomized controlled trial; SAE, serious AE; SGLT2i, sodium-glucose cotransporter-2 inhibitor.</p

H₂O₂-induced PSMD6 nuclear import is impaired in the absence of uPAR.

<p>A. SiCo and uPARsi -nucleofected human VSMC were treated with 100 µM H₂O₂ for 1 h at 37°C. Then cells were fixed and stained for PSMD6 (Alexa 488) DraQ5 was used as nuclear stain. B. Human VSMC were treated as in A and subcellular fractionation was performed. PSMD6 content in nuclear fraction was assessed by western blotting. Histon H3 was used as loading control. Scale bar 100 µm. C. H₂O₂ -induced nuclear import of PSMD6 was quantified from 3 independent experiments.</p

DNA SSB signaling and DNA repair are impaired in uPARsi cells.

<p>A. SiCo and uPARsi -nucleofected human VSMC were treated with 100 µM H₂O₂ for indicated time points. Phosphorylation of ATR and Chk1 kinase was detected by western blotting. B. HEK 293 cells were infected with control lentivirus or uPAR-FLAG-expressing virus, and stimulated with 100 µM H₂O₂ for indicated time points. Phosphorylation of Chk1 and Chk2 kinases was detected by western blotting. C. WT and uPAR−/− mouse VSMC were treated with H₂O₂ for 20 min on ice to induce DNA damage. After H₂O₂ removal VSMC were allowed to repair DNA for 4 hrs. Comet tails were quantified as described in the Materials and Methods. D. WT and uPAR−/− mouse VSMC were treated with different concentrations of H₂O₂ for 20 min on ice to induce DNA damage. The number of viable cells was calculated 24 hrs after DNA damage using 5(6)CFDA as described in Material and methods.</p

H₂O₂ induces DNA DSB signaling in WT and uPAR−/− mouse VSMC.

<p>VSMC isolated from WT (A) and uPAR−/− (B) mice were treated with 100 µM H₂O₂ for indicated time. Phosphorylation of ATM, Chk-2 and H2AX was assessed by western blotting. C. H₂O₂-induced phosphorylation of ATM, Chk-2 and H2AX was quantified from 3 independent experiments. Data are shown as folds of increase relative to unstimulated control and normalized to the total level of corresponding protein.</p

MMS-induced increase of proteasome activity and PSMD6 nuclear import are abrogated in uPARsi VSMC.

<p>A. SiCo and uPARsi human VSMC were treated with 1.2(B) and uPARsi (C) VSMC were treated with 1.2 mM MMS for 1 h, then fixed and stained for PSMD6 (Alexa 488) and 20Sα7 subunit (Alexa 594). DraQ 5 was used as nuclear stain. The right panels in B, C show colocalization of PSMD6 and 20Sα7 indicated by color coding. The colormap was created using colocalization colormap plugin of ImageJ software. Scale bar 100 µm.</p