4 research outputs found

    Frataxin protein and mRNA level is stable from day-to-day in whole blood.

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    <p>(A) Frataxin protein levels measured by dipstick assay in FRDA patient subjects over the course of 4 weeks (Cohort 1; nβ€Š=β€Š31). (B) Frataxin protein levels measured by dipstick assay in whole blood collected from 5 FRDA patients (red) and 5 related carriers (blue) weekly for 15 weeks (Cohort 2). (C) Frataxin mRNA levels measured by RT-qPCR in blood collected in PAXgene tubes from Cohort 2. Frataxin mRNA level is expressed relative to control donor levels and is normalized to the endogenous control gene <i>GAPDH</i>.</p

    Frataxin protein and mRNA levels strongly correlated when measured in different cellular compartments (Cohort 3).

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    <p>(A) Frataxin mRNA in PBMCs and whole blood (R<sup>2</sup>β€Š=β€Š0.84, p<0.0001). (B) Frataxin protein in PBMCs and whole blood (R<sup>2</sup>β€Š=β€Š0.63, p<0.0001). (C) Frataxin mRNA and protein in whole blood (R<sup>2</sup>β€Š=β€Š0.55, p<0.0001). (D) Frataxin mRNA and protein in PBMCs (R<sup>2</sup>β€Š=β€Š0.58, p<0.0001). (E) Frataxin protein in PBMCs and cheek swabs (R<sup>2</sup>β€Š=β€Š0.67, p<0.0001). (F) Frataxin protein in whole blood and cheek swabs (R<sup>2</sup>β€Š=β€Š0.58, p<0.0001). Frataxin mRNA level in PBMCs and whole blood is expressed relative to control donor levels and is normalized to the endogenous control gene <i>GAPDH</i>.</p
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