FORMULASI DAN UJI ANTI BAKTERI SEDIAAN GEL ANTIJERAWAT EKSTRAK HERBA SURUHAN (Peperomia pellucida L. Kunth.) TERHADAP BAKTERI Propionibacterium acnes

Jerawat (Acne vulgaris) adalah kondisi peradangan pada folikel sebaceous yang dapat menyerang pria dan wanita pada segala usia. Jerawat dapat disebabkan oleh berbagai keadaan, dan infeksi bakteri Propionibacterium acnes adalah salah satu penyebabnya. Propionibacterium acnes termasuk mikroflora yang terdapat pada kulit manusia, namun jika jumlahnya berlebih maka dapat menyebabkan jerawat. Pengobatan jerawat yang umum dilakukan adalah dengan menggunakan antibiotik atau berbagai produk sintetik/kimia yang beredar di pasaran. Penggunaan obat jerawat sintetik dapat menyebabkan terjadinya resistensi, iritasi, kerusakan organ, hingga hipersensitivitas imun. Oleh karena itu peneliti melakukan penelitian pemanfaatan bahan alam yaitu ekstrak herba Suruhan (Peperomia pellucida L. Kunth) sebagai alternatif bahan alam yang diformulasi dalam bentuk sediaan gel untuk mengatasi masalah jerawat. Gel dengan konsentrasi ekstrak 25% memiliki aktivitas antibakteri terbaik. Hasil evaluasi sediaan gel dengan konsentrasi ekstrak 25% menunjukkan karakteristik yang baik dan sesuai dengan standar sediaan topikal. Evaluasi sediaan gel yang dilakukan meliputi pemeriksaan pH, organoleptik, homogenitas, dan daya sebar. Hasil analisis statistik menggunakan One way ANOVA  menunjukkan terdapat perbedaan yang bermakna (P<0,05) antara konsentrasi ekstrak yang digunakan dalam sediaan gel dengan penghambatan pertumbuhan bakteri penyebab jerawat  Propionibacterium acnes

Sitotoksisitas dan Induksi Apoptosis Ekstrak Etanol Teripang holothuria atra Jaeger, 1833 pada beberapa Sel Kanker

Teripang Holothuria atra merupakan biota laut yang banyak ditemukan di perairan Indonesia yang termasuk dalam filum Echinodermata dan berpotensi sebagai antikanker. Penelitian ini bertujuan untuk mengetahui sitotoksisitas dan induksi apoptosis ekstrak etanol teripang H. atra secara in vitro terhadap beberapa sel lestari. Pengujian sitotoksisitas dilakukan dengan metode MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) menggunakan sel HeLa, T47D, WiDr dan sel normal Vero, sedangkan uji induksi apoptosis dilakukan terhadap sel dengan hasil uji sitotoksisitas terbaik menggunakan metode flowcytometry dan double staining. Hasil penelitian menunjukkan bahwa ekstrak etanol  teripang H. atra mampu menghambat pertumbuhan sel kanker HeLa, T47D dan WiDr. Nilai IC50 ekstrak H. atra terhadap ketiga sel tersebut masing-masing sebesar 41,06±4,21; 20,89±1,55; 26,50±4,43 µg/ml tetapi esktrak tersebut memiliki sitotoksisitas  yang lebih rendah terhadap sel Vero (IC50 sebesar 128,00). Analisis flowcytometry dan double staining pada sel T47D memperlihatkan bahwa ekstrak etanol teripang H.atra mampu menginduksi apoptosis pada sel tersebut

Anticancer Activity of Turmeric Rhizome Extract (Curcuma longa Linn) In-vitro Against MCF7 Breast Cancer Line Cells

Turmeric or Curcuma longa Linn is one of the native spices and medicinal plants from Southeast Asia. Turmeric contains a compound named curcumin. Curcumin (diferuloylmethane) is a yellow pigment in turmeric that is widely used as a spice, food coloring (curry) and preservative. Curcumin shows various pharmacological effects that have been reported scientifically in research, one of which is as an anticancer. This study aims to perform a cytotoxic test of turmeric rhizome extract (Curcuma longa Linn) in-vitro with the Microtetrazolim (MTT) assay method to grasp and determine the effect of preventing the growth of MCF7 breast cancer cells, so that the IC50 value can be known. In this study, it was found that turmeric rhizome extract can be used as a natural ingredient that has the potential to inhibit the growth of MCF7 breast cancer cells. Turmeric rhizome extract has the effectiveness to inhibit the growth of MCF7 breast cancer cells with an IC50 value of 26.30 ppm. This value is categorized as moderate or moderately active cytotoxic.Turmeric or Curcuma longa Linn is one of the native spices and medicinal plants from Southeast Asia. Turmeric contains a compound named curcumin. Curcumin (diferuloylmethane) is a yellow pigment in turmeric that is widely used as a spice, food coloring (curry) and preservative. Curcumin shows various pharmacological effects that have been reported scientifically in research, one of which is as an anticancer. This study aims to perform a cytotoxic test of turmeric rhizome extract (Curcuma longa Linn) in-vitro with the Microtetrazolim (MTT) assay method to grasp and determine the effect of preventing the growth of MCF7 breast cancer cells, so that the IC50 value can be known. In this study, it was found that turmeric rhizome extract can be used as a natural ingredient that has the potential to inhibit the growth of MCF7 breast cancer cells. Turmeric rhizome extract has the effectiveness to inhibit the growth of MCF7 breast cancer cells with an IC50 value of 26.30 ppm. This value is categorized as moderate or moderately active cytotoxic

Anticancer Activity of Turmeric Rhizome Extract (Curcuma longa Linn) In-vitro Against MCF7 Breast Cancer Line Cells

Turmeric or Curcuma longa Linn is one of the native spices and medicinal plants from Southeast Asia. Turmeric contains a compound named curcumin. Curcumin (diferuloylmethane) is a yellow pigment in turmeric that is widely used as a spice, food coloring (curry) and preservative. Curcumin shows various pharmacological effects that have been reported scientifically in research, one of which is as an anticancer. This study aims to perform a cytotoxic test of turmeric rhizome extract (Curcuma longa Linn) in-vitro with the Microtetrazolim (MTT) assay method to grasp and determine the effect of preventing the growth of MCF7 breast cancer cells, so that the IC50 value can be known. In this study, it was found that turmeric rhizome extract can be used as a natural ingredient that has the potential to inhibit the growth of MCF7 breast cancer cells. Turmeric rhizome extract has the effectiveness to inhibit the growth of MCF7 breast cancer cells with an IC50 value of 26.30 ppm. This value is categorized as moderate or moderately active cytotoxic.Turmeric or Curcuma longa Linn is one of the native spices and medicinal plants from Southeast Asia. Turmeric contains a compound named curcumin. Curcumin (diferuloylmethane) is a yellow pigment in turmeric that is widely used as a spice, food coloring (curry) and preservative. Curcumin shows various pharmacological effects that have been reported scientifically in research, one of which is as an anticancer. This study aims to perform a cytotoxic test of turmeric rhizome extract (Curcuma longa Linn) in-vitro with the Microtetrazolim (MTT) assay method to grasp and determine the effect of preventing the growth of MCF7 breast cancer cells, so that the IC50 value can be known. In this study, it was found that turmeric rhizome extract can be used as a natural ingredient that has the potential to inhibit the growth of MCF7 breast cancer cells. Turmeric rhizome extract has the effectiveness to inhibit the growth of MCF7 breast cancer cells with an IC50 value of 26.30 ppm. This value is categorized as moderate or moderately active cytotoxic

Pengujian In Vitro Serum Antiacne Kombinasi Ekstrak Daun Kemangi dan Rimpang Kunyit

Abstract—Acne is a chronic inflammation of the pilosebaceous follicles which is characterized by excess oil production caused by the bacterium Propionibacterium acnes. The content of flavonoids in basil leaves and the content of curcumin in turmeric rhizome can be used as an anti-acne treatment. The purpose of this study was to determine the anti-acne activity of a single extract of basil leaves and a single extract of turmeric rhizome, to determine the ability of the combination two extracts to inhibit acne-causing bacteria and to formulate combination of basil leaf extract and turmeric rhizome into a serum preparation to determine antibacterial activity against Propionibacterium acnes. The research method used was experimental by testing the antibacterial activity of Propionibacterium acnes in vitro. Antibacterial activity test results in serum showed a negative control, namely serum base did not have an inhibition zone, positive control clindamycin phosphate showed an inhibition zone of 13.20 mm, with a ratio between extracts of turmeric rhizome and basil leaves 1:4, the diameter of the inhibition zone was obtained at a concentration of 30 % of 5.7 mm, 40% concentration of 7.38 mm and 50% concentration of 8.11 mm. Statistical analysis was carried out using the Shapiro-Wilk, Levene Statistics, and One Way Anova methods (p&lt;0.05). Keywords: acne, basil, p. acnes, serum, turmeric &nbsp; Abstrak—Jerawat merupakan peradangan kronik folikel pilosebasea yang ditandai dengan adanya produksi minyak berlebih yang disebabkan oleh bakteri Propionibacterium acnes. Kandungan flavonoid pada daun kemangi dan kandungan kurkumin pada rimpang kunyit dapat dimanfaatkan sebagai pengobatan antijerawat. Sediaan farmasi yang dapat digunakan untuk mengatasi masalah kulit berjerawat yaitu serum. Tujuan penelitian ini adalah mengetahui aktivitas antijerawat pada ekstrak tunggal daun kemangi dan ekstrak tunggal rimpang kunyit, mengetahui kemampuan kombinasi kedua ekstrak dalam menghambat bakteri penyebab jerawat dan memformulasikan kombinasi ekstrak daun kemangi dan rimpang kunyit menjadi sediaan serum untuk mengetahui aktivitas antibakteri terhadap Propionibacterium acnes. Metode penelitian yang digunakan adalah eksperimental dengan melakukan uji aktivitas antibakteri Propionibacterium acnes secara in vitro. Hasil uji aktivitas antibakteri pada serum menunjukkan kontrol negatif yaitu basis serum tidak mempunyai zona hambat, kontrol positif clindamycin phospat menunjukkan zona hambat sebesar 13,20 mm, dengan perbandingan antara ekstrak rimpang kunyit dan daun kemangi 1:4 diperoleh hasil diameter zona hambat pada konsentrasi 30% sebesar 5,7 mm, konsentrasi 40% sebesar 7,38 mm dan konsentrasi 50% sebesar 8,11 mm. Analisa statistik dilakukan dengan metode Shapiro-wilk, Levene statistic, dan One Way Anova (p&lt;0,05). Kata kunci: jerawat, kemangi, kunyit, p. acnes, seru

A network pharmacology approach to elucidate the anti-inflammatory and antioxidant effects of bitter leaf (Vernonia amygdalina Del.)

The therapeutic potential of bitter leaf (Vernonia amygdalina Del.) has been established both empirically and in various scientific investigations. However, the molecular pathways related to its possible anti-inflammatory and antioxidant properties remain unclear. Therefore, the aim of this study was to elucidate the molecular interactions between bitter leaf's bioactive compounds and cellular targets involved in these activities. The compounds in bitter leaf were identified using gas chromatography-mass spectrometry (GC-MS) analysis, and subsequently, a network pharmacology approach was employed together with molecular docking and dynamics simulations. Acetonitrile (4.5%) and dimethylamine (4.972%) were the most prevalent compounds among the 38 identified by the GC-MS analysis of bitter leaf extract. The proto-oncogene tyrosine-protein kinase (SRC) demonstrated significant connectivity within the antioxidant network, highlighting its pivotal role in facilitating inter-protein communication. It also exhibited strategic positioning in anti-inflammatory mechanisms based on closeness centrality (0.385). The enrichment analysis suggested multifaceted mechanisms of bitter leaf compounds, including transcriptional regulation and diverse cellular targeting, indicating broad antioxidant and anti-inflammatory effects. Eicosapentaenoyl ethanolamide (EPEA) displayed strong interactions with multiple proteins, including SRC (-7.17 kcal/mol) and CYP3A4 (-6.88 kcal/mol). Moreover, EPEA demonstrated to form a stable interaction with SRC during a 100 ns simulation. In conclusion, the computational simulations revealed that the hypothetical antioxidant and anti-inflammatory actions of bitter leaf compounds were achieved by specifically targeting SRC. However, confirmation using either in vitro or in vivo techniques is necessary

Workshop Pembuatan Snack Makanan Sehat Pencegah Penyakit Degeneratif Kepada Warga Kelurahan Sukamaju Cilodong Kota Depok

Penyakit degeneratif adalah suatu kondisi kesehatan dimana organ atau jaringan tubuh dalam keadaan yang terus menurun dari waktu ke waktu.&nbsp; Kurangnya aktivitas fisik, gaya hidup, dan pola makan merupakan faktor yang berkontribusi terhadap peningkatan angka kejadian penyakit degeneratif.&nbsp; Kegiatan penyuluhan di Kelurahan Sukamaju RT 06/ RW 10 dengan tahapan meliputi perizinan tempat, penyewaan perlengkapan dan peralatan, sosialisasi, penyuluhan dan penutup. Menu yang akan di demo masakan yang disampaikan yaitu “Stick Bayamâ€, “Jus Seledriâ€, “Dimsum Lele". Kegiatan Demo Masak Pembuatan Makanan Ringan dan Minuman Sehat merupakan upaya penting untuk meningkatkan pemahaman dan kesadaran ibu PKK RT 06 dan RW 10 kelurahan yang sebagian besar adalah ibu rumah tangga yang memiliki peran untuk menjaga asupan gizi dalam makanan pada keluarga untuk mencegah penyakit degeneratif. Kegiatan ini dimaksudkan agar masyarakat dapat mengubah asupan pola makan untuk mencegah terjadinya penyakit degeneratif sejak dini dan dapat memenuhi nutrisi