Additional file 1: Table S1. of A set of genes conserved in sequence and expression traces back the establishment of multicellularity in social amoebae

Gene set: The genes identified and additional information on orthology, detection method, and expression peak. (XLSX 97 kb

Additional file 2: Table S3. of A set of genes conserved in sequence and expression traces back the establishment of multicellularity in social amoebae

Oligonucleotides primer sequences for knock out studies. (DOC 56 kb

Additional file 4: Table S2. of A set of genes conserved in sequence and expression traces back the establishment of multicellularity in social amoebae

Upregulated mutants upregulated mutant list and ko phenotype description. (XLSX 22 kb

Additional file 3: of A set of genes conserved in sequence and expression traces back the establishment of multicellularity in social amoebae

Supplementary Figures 1-6. (DOCX 3657 kb

MOESM1 of Phylogeny-wide conservation and change in developmental expression, cell-type specificity and functional domains of the transcriptional regulators of social amoebas

Additional file 1: Figure S1-S18. Annotated phylogenetic trees of transcription factor families

MOESM3 of Phylogeny-wide conservation and change in developmental expression, cell-type specificity and functional domains of the transcriptional regulators of social amoebas

Additional file 3: Table S2. Transcription factor conservation. Conservation and change in the presence, developmental expression and functional domain architecture in transcription factors across five Dictyostelid genomes

MOESM4 of Phylogeny-wide conservation and change in developmental expression, cell-type specificity and functional domains of the transcriptional regulators of social amoebas

Additional file 4: Table S3. Transcription factors grouped by cell-type and stage specificity. The data of Additional file 3: Table S2 were compiled and sorted to generate sets that were expressed in the same cell type or at the same developmental stage, or that shared a combination of the same stage and cell type specificity

Additional file 2: Figure S3. of Improved annotation with de novo transcriptome assembly in four social amoeba species

Annotated domain diagrams for 16 D. discoideum developmentally relevant proteins which have had their protein sequence altered. (PDF 367 kb

MOESM5 of Phylogeny-wide conservation and change in developmental expression, cell-type specificity and functional domains of the transcriptional regulators of social amoebas

Additional file 5: Table S4. Transcription factors with known knock-out phenotypes. Knock-out phenotypes of transcription factors combined with their cell-type- and stage specificity of expression

Additional file 1: Figure S1. of Improved annotation with de novo transcriptome assembly in four social amoeba species

TAGC plot for D. fasciculatum (A) and D. lacteum (B) before and after filtering. Each colour blob represents different taxa with unmatched transcripts are shaded in grey. The unannotated grey coloured transcripts after filtration set further filtered by high GC and low read coverage. This plot shows a major blob of transcripts that are annotated with the Dictyostelium fasciculatum species with high coverage and lower GC content. Other contaminations form E.coli, pseudomonas fluorescence and other species has also been highlighted with different colours. These contaminations clearly make different blobs with lower read coverage and high GC content. However, it’s good to see that there are some other transcripts that showing matched to dictyostelium discoideum- that clearly reflect the presence of some novel unannotated transcripts in the new assembly. Figure S2 A comparison of assembled transcripts read count. The boxplots represent the range between the 1st and 3rd quartiles of the data by the coloured boxes, the median is the horizontal bar and points shown beyond the whiskers are >95% of the data. Table S3 Transrate good contigs. Table S4 Olignucleotide sequences. Table S5 Alignment with DNA sequence of PCR product. (DOCX 787 kb