Sensitivity-Tunable Terahertz Liquid/Gas Biosensor Based on Surface Plasmon Resonance with Dirac Semimetal

In this paper, we study the sensitivity-tunable Terahertz (THz) liquid/gas biosensor in a coupling prism-three-dimensional Dirac semimetal (3D DSM) multilayer structure. The high sensitivity of the biosensor originates from the sharp reflected peak caused by surface plasmon resonance (SPR) mode. This structure achieves the tunability of sensitivity due to that the reflectance could be modulated by the Fermi energy of 3D DSM. Besides, it is found that the sensitivity curve depends heavily on the structural parameters of 3D DSM. After parameter optimization, we obtained sensitivity over 100{\deg}/RIU for liquid biosensor. We believe this simple structure provides a reference idea for realizing high sensitivity and tunable biosensor device

Tunable nonlinear optical bistability based on Dirac semimetal in photonic crystal Fabry-Perot cavity

In this paper, we study the nonlinear optical bistability (OB) in a symmetrical multilayer structure. This structure is constructed by embedding a nonlinear three-dimensional Dirac semimetal (3D DSM) into a solution filled one-dimensional photonic crystal Fabry-Perot cavity. OB stems from the third order nonlinear conductivity of 3D DSM and the local field of resonance mode could enhance the nonlinearity and reduce the thresholds of OB. This structure achieves the tunability of OB due to that the transmittance could be modulated by the Fermi energy. OB threshold and threshold width could be remarkably reduced by increasing the Fermi energy. Besides, it is found that the OB curve depends heavily on the angle of incidence of the incoming light, the structural parameters of the Fabry-Perot cavity, and the position of 3D DSM inside the cavity. After parameter optimization, we obtained OB with a threshold of 106 V/m. We believe this simple structure provides a reference idea for realizing low threshold and tunable all optical switching devices. Keywords: Optical bistability, Dirac semimetal, Fabry-Perot cavity

Additional file 1: of Oil body bound oleosin-rhFGF9 fusion protein expressed in safflower (Carthamus tinctorius L.) stimulates hair growth and wound healing in mice

Table R1. Culture medium composition of safflower. (PDF 22 kb

TAT-rhbFGF and rhbFGF penetrated into the skin of a mouse.

<p>The nuclei stained blue, and the positive proteins stained brown.</p

Agarose gel electrophoresis results from the identified recombinant gene.

<p>In section a, lane 1 is the product of the first PCR step; in section b, lane 1 is the final recombinant gene; in section c, lane 1 is the pET3c plasmid vector, lane 2 is the PCR product of the transformant, and lane 3 is the digested recombinant vector product.</p

Effect of TAT-rhbFGF on the index of hypertrophic scar thickness.

<p>Note: Data are presented as the mean±SD.</p><p>* Compared with the control, <i>P</i><0.05.</p><p>Effect of TAT-rhbFGF on the index of hypertrophic scar thickness.</p

The proliferation activity of TAT-rhbFGF was tested using NIH 3T3 Cells.

<p>NIH3T3 cells, which were seeded into 96-well microplates at 7000 cells/well, were allowed to attach (4–6 hours) and were then incubated in DMEM with 0.5% fetal bovine serum overnight at 37°C. Cells were supplemented with rhbFGF and TAT-rhbFGF and were incubated at 37°C for 48 hours. PBS was used as a control.</p

The ability of TAT-rhbFGF to penetrate cells.

<p>These images were produced using immunofluorescence. The target protein was identified by green fluorescence.</p

The effect of rhbFGF and TAT-rhbFGF gel on the hypertrophic scar model.

<p>(A and C): the tissue slices of the hypertrophic scar stained using H&E, A: observed at 400×, C: the relative density of fibroblasts; all the data are compared with the mean density of the normal derma. * Compared with the control, <i>P</i><0.05; (B and D): the content of type I and III collagen in the scars, B: the collagen stained using a Masson kit, observed at 200× D: the relative content of type I and III collagen; all the data are compared with the mean content of the normal derma. *<i>P</i><0.05 and ***<i>P</i><0.01 compared with the control; (E): the apoptosis cells in HTS after treatment observed at 200×.</p

The purification and identification of TAT-rhbFGF.

<p>In section a, lane 1 is the supernatant of the cell lysate, lane 2 is the purified product from the CM Sepharose, and lane 3 is the final product of the heparin sepharose chromatography. In section b, lane 1 and lane 2 are the western blot results for rhbFGF and TAT-rhbFGF, respectively.</p