Construction of a rabbit limbal stem cell deficiency model.

<p>Representative slit-lamp photograph of rabbit cornea showed significant opacity and neovascularization after 1 month (A). PAS staining in which goblet cells are easily observed in corneal impression cytology (B). Signet ring goblet cells (arrow) were also revealed by H.E. staining of corneal sections (C). Scale bar: 10 µm (B) and 50 µm (C).</p

Scores of the corneal surface over time in all groups (corneal haze/neovascularization/sum).

<p>Scores of the corneal surface over time in all groups (corneal haze/neovascularization/sum).</p

Construction of rabbit corneal epithelium tissue-engineered with acellular conjunctiva matrix.

<p>Rabbit primary limbal epithelial cells proliferated well at day 7 (A). Trypan blue-alizarin red staining showed that the cells maintained live activity and grew to confluence (B). The cells could be labeled with CM-Dil (red fluorescence) (C) and stained positive with anti-K3/12 (D). Negative control lacks primary antibody (E). The rabbit corneal epithelial cells formed a 2–3 epithelial layer structure after culture for 14 days, as confirmed by H.E. staining (F) and TEM observation (G). There were tight junctions between cells and the aCM scaffold (H). Scale bar: 100 µm (D, E), 50 µm (B, C, F), 5 µm (G) and 1 µm (H).</p

Corneal H and E staining and impression cytology after transplantation of tissue-engineered corneal epithelium.

<p>Restoration of corneal epithelium began at day 7 after transplantation with reconstructed corneal epithelium on aCM (A). Normal morphology was observed at day 30 (B) with no goblet cells (C), whereas the group that received reconstructed corneal epithelium on dAM exhibited defective corneal epithelium at day 7 (D) and goblet cells (arrow) were still observed at day 30 (E, F). Scale bar: 100 µm (A, B, D, E) and 10 µm (C, F).</p

The effect of acellular conjunctiva matrix extracts on the proliferation of human corneal epithelial cells.

<p>The effect of acellular conjunctiva matrix extracts on the proliferation of human corneal epithelial cells.</p

Postoperative tracking of donor cells on the recipient cornea.

<p>More donor cells were detected on peripheral region of the rabbit cornea transplanted with aCM (A) compared with that transplanted with dAM (B) at days 7 and 30. At day 30, more donor cells had migrated into the central cornea region transplanted with aCM-based corneal epithelium (A), whereas few cells were detected in the central cornea transplanted with dAM-based corneal epithelium (B). Scale bar: 100 µm.</p

Fast Photoconductive Responses in Organometal Halide Perovskite Photodetectors

Inorganic semiconductor-based photodetectors have been suffering from slow response speeds, which are caused by the persistent photoconductivity of semiconductor materials. For realizing high speed optoelectronic devices, the organometal halide perovskite thin films were applied onto the interdigitated (IDT) patterned Au electrodes, and symmetrical structured photoconductive detectors were achieved. The detectors were sensitive to the incident light signals, and the photocurrents of the devices were 2–3 orders of magnitude higher than dark currents. The responsivities of the devices could reach up to 55 mA W^1–. Most importantly, the detectors have a fast response time of less than 20 μs. The light and bias induced dipole rearrangement in organometal perovskite thin films has resulted in the instability of photocurrents, and Ag nanowires could quicken the process of dipole alignment and stabilize the photocurrents of the devices

Biocompatibility of acellular conjunctiva matrix in vivo.

<p>Representative slit-lamp photographs just after intracorneal transplantation (A) and one month later (B). H.E. staining showed that transplanted aCM (arrow) adapted well to the host corneal stroma, with no evidence of inflammatory cells or stromal edema. Scale bar: 100 µm (C) and 50 µm (D).</p

Macroscopic view of the acellular conjunctiva matrix.

<p>The aCM (B, E) were more transparent than the normal conjunctiva matrix (A, D). The transparent characteristics were not affected after the sterilization with γ-irradiation (C, F).</p

Hierarchically Structured Porous Nitrogen-Doped Carbon for Highly Selective CO₂ Capture

Nitrogen-doping has proven to be an effective strategy for enhancing the CO₂ adsorption capacity of carbon-based adsorbents. However, it remains challenging to achieve a high doping level of nitrogen (N) and a significant porosity in a carbon material simultaneously. Here we report a facile method that enables the fabrication of ordered macroporous nitrogen-doped carbon with the content of N as high as 31.06 wt %. Specifically, we used poly­(EGDMA-<i>co</i>-MAA) microspheres as a template to fabricate the structure which can strongly interact with melamine (the precursor of nitrogen-doped carbon framework), self-assemble into three-dimensionally ordered structure, and be easily removed afterward. Upon chemical activation, significant microporosity is generated in this material without degrading its ordered macroporous structure, giving rise to a hierarchically structured porous nitrogen-doped carbon in which a remarkable N content (14.45 wt %) is retained. This material exhibits a moderate CO₂ adsorption capacity (2.69 mmol g^–1 at 25 °C and 3.82 mmol g^–1 at 0 °C under 1 bar) and an extraordinarily high CO₂/N₂ selectivity (134), which is determined from the single-component adsorption isotherms based on the ideal adsorption solution theory (IAST) method. This value far exceeds the CO₂/N₂ selectivity of thus-far reported carbon-based adsorbents including various nitrogen-doped ones. We believe that such an unprecedented CO₂/N₂ selectivity is largely associated with the unusually high N content as well as the partially graphitic framework of this material