A highly sensitive and specific system for large-scale gene expression profiling-1

Ells were plotted against those of a duplicated 100-cell sample, and , a scatter plot in after replacing the data of the latter 100-cell sample with data of a 10,000-cell sample from the same cell line. Microarray intensities shown in the figure are the results after baseline subtraction. Both plots are on the log scale.<p><b>Copyright information:</b></p><p>Taken from "A highly sensitive and specific system for large-scale gene expression profiling"</p><p>http://www.biomedcentral.com/1471-2164/9/9</p><p>BMC Genomics 2008;9():9-9.</p><p>Published online 10 Jan 2008</p><p>PMCID:PMC2267712.</p><p></p

A highly sensitive and specific system for large-scale gene expression profiling-3

<p><b>Copyright information:</b></p><p>Taken from "A highly sensitive and specific system for large-scale gene expression profiling"</p><p>http://www.biomedcentral.com/1471-2164/9/9</p><p>BMC Genomics 2008;9():9-9.</p><p>Published online 10 Jan 2008</p><p>PMCID:PMC2267712.</p><p></p

A highly sensitive and specific system for large-scale gene expression profiling-2

N profiling results by gel assay. Quantitative results are given in Table 3. M, MCF-7, and A, NCI/ADR-RES.<p><b>Copyright information:</b></p><p>Taken from "A highly sensitive and specific system for large-scale gene expression profiling"</p><p>http://www.biomedcentral.com/1471-2164/9/9</p><p>BMC Genomics 2008;9():9-9.</p><p>Published online 10 Jan 2008</p><p>PMCID:PMC2267712.</p><p></p

A highly sensitive and specific system for large-scale gene expression profiling-0

<p><b>Copyright information:</b></p><p>Taken from "A highly sensitive and specific system for large-scale gene expression profiling"</p><p>http://www.biomedcentral.com/1471-2164/9/9</p><p>BMC Genomics 2008;9():9-9.</p><p>Published online 10 Jan 2008</p><p>PMCID:PMC2267712.</p><p></p

Effect of LiFSI Concentrations To Form Thickness- and Modulus-Controlled SEI Layers on Lithium Metal Anodes

Improving the cyclic stability of lithium metal anodes is of particular importance for developing high-energy-density batteries. In this work, a remarkable finding shows that the control of lithium bis­(fluorosulfonyl)­imide (LiFSI) concentrations in electrolytes significantly alters the thickness and modulus of the related SEI layers, leading to varied cycling performances of Li metal anodes. In an electrolyte containing 2 M LiFSI, an SEI layer of ∼70 nm that is obviously thicker than those obtained in other concentrations is observed through <i>in situ</i> atomic force microscopy (AFM). In addition to the decomposition of FSI^– anions that generates rigid lithium fluoride (LiF) as an SEI component, the modulus of this thick SEI layer with a high LiF content could be significantly strengthened to 10.7 GPa. Such a huge variation in SEI modulus, much higher than the threshold value of Li dendrite penetration, provides excellent performances of Li metal anodes with Coulombic efficiency higher than 99%. Our approach demonstrates that the FSI^– anions with appropriate concentration can significantly alter the SEI quality, establishing a meaningful guideline for designing electrolyte formulation for stable lithium metal batteries

Rare variants identified in <i>DLC1</i> isoform 1.

<p>(A) The locations of the rare variants are indicated by black lines on the DLC1 isoform 1 protein. FAT (focal adhesion targeting) region, SAM (sterile alpha motif), Rho-Gap (Rho-GTPase-activating protein) and START (steroidogenic acute regulatory protein related lipid transfer) domains are indicated by different colors. Stars denote the private variants identified in the CHD cohort. (B) DLC1 isoform 1 possesses an extended N-terminal region compared to isoform 2. The first 437 residues of isoform 1 are missing in isoform 2, and the sequence ‘TAIQGISEKEKAE’ is replaced by ‘MCRKKPDTMILTQ’ in isoform 2. The yellow box indicates the SAM domain in DLC1, and the green box shows the N-terminal region. (C) The conservation of residues in the N-terminal region was analyzed in different species. The primates and non-primates are separated by the blue lines in the boxes. Asterisks indicate the residues that are conserved among the primates. The residues that are conserved in the primates and non-primates locate in the red boxes. The UniProt accession ID is followed by a colon and the corresponding species name. (D) The private variants that altered the regulation of cell migration function of DLC1 are shown.</p

<i>DLC1</i> isoform 1 mutants had different effects on cell migration compared with the wild type protein.

<p>(A) Western blot analyses of <i>DLC1</i> isoform 1 mutant overexpression in two endothelial cell lines, HUVEC and HBMEC-60. In HUVECs, the effect of <i>DLC1</i> isoform 1 mutation on the GAP activity of the protein was detected by western blotting. (B) Representative images of the Transwell migration assay using HUVECs cells are shown. (C) The quantification of HUVEC and HBMEC-60 migration showed significant differences between wild-type DLC1 and Mutants 2, 4 and 5, whereas the other mutants showed no significant difference from wild-type DLC1. Wild-type DLC1 also showed an inhibitory effect on cell migration compared to the control vector. *Student's t-test ; ** . Scale bars, 100 µm. Ns, not significant.</p

The subcellular localization of wild-type DLC1 and mutants in HUVECs.

<p>(A) Images of wild-type and mutant DLC1 distribution in HUVECs using laser scanning confocal microscopy. (B) The percentage of cells with wild-type and mutant DLC1 proteins with exclusive cytoplasmic-localization. Mutant 4 showed a significant difference from the wild type, as opposed to the control vector. *Student's t-test ; ** . Scale bar, 25 µm. Ns, not significant.</p

Comparison between data from microarray and gel electrophoresis.

<p>Meanings of the x- and y-axes in all scatter plots are the same as those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005236#pone-0005236-g002" target="_blank">Figure 2</a>.</p

Correlation between genotypes of the donors and their sperm samples for CNV Rs2287968.

<p>Meanings of the graphics are the same as those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005236#pone-0005236-g002" target="_blank">Figure 2</a>.</p