Quality and completeness scores for curated and non-curated datasets

These data contain aggregated survey responses assessing the quality and completeness of metadata for datasets deposited in public repositories and for the same datasets after professional curation.<div><div><br></div><div>Responses were provided by 10 professional editors representing life, social and physical sciences. Each were randomly assigned four datasets to assess, half (20) of which had been curated according to the standards of Springer Nature's Research Data Support service and half (20) which had not.</div><div><br></div><div>Curated datasets were shared privately with research participants. The versions that did not receive curation via Springer Nature's Research Data Support are openly accessible.<br></div><div><br></div><div>Single-blind testing was employed; the researchers were not made aware which datasets had been curated and which had not, and it was ensured that no participant assessed the same dataset before and after curation. Responses were collected via an online survey. The relevant question and scoring is provided below:<br></div><div><br></div><div><i>Rate the overall quality and completeness of the metadata for the dataset (with regards to finding and accessing and citing the data, not reusing the data)1 = not complete, 5 = very complete</i></div></div

Preparation and Crystal Structure of the Silver(I) <i>p</i>-Toluenesulfonate−<i>p</i>-Aminobenzoic Acid Complex Polymer Adduct, the First Reported Example of a Silver(I) Complex Involving Two Different Organic Acid Species

Preparation and Crystal Structure of the Silver(I) p-Toluenesulfonate−p-Aminobenzoic Acid Complex Polymer Adduct, the First Reported Example of a Silver(I) Complex Involving Two Different Organic Acid Specie

Crystal structure of Ku 80.

<p>Images of the front (top) and back (bottom) of the Ku 80 protein displaying the DNA binding domain (yellow), surface cysteines (blue), Cys-493 (red) and Cys-249 (pink).</p

Percentage of transient Foci.

<p>These are foci that disappeared and then reformed rather than shrunk to a small size and then regrew. Ten unstressed cells (53 Foci) and 6 stressed cells (135 Foci) were observed in total. Results are presented as mean ± SD.</p

Signalling of DNA double strand breaks is done by the phosphorylation of the histone H2AX and the formation of a Damage Focus around the DSB.

<p>Phosphorylation of H2AX is caused by autophosphorylation of ATM and DNA-PKcs at the site of damage.</p

Repair Mechanisms of Non-Homologous End Joining.

<p>(A) The primary repair pathway of DSB repair by NHEJ is mediated by a hetrodimer DNA-PK which is made up of Ku70, Ku 80 and DNA-PKcs and is commonly named DNA-PK Dependant Non-Homologous End Joining (D-NHEJ). Once the DNA-PK has formed a complex with the site of the DSB the break is readied for repair by ligation from the Enzyme LiIV which is in complex with XRCC4. (B) A second NHEJ pathway called Backup Non-Homologous End Joining (B-NHEJ) mediated by PARP-1 also exists. Once the break is primed by the formation of the DSB-PARP complex, the broken ends are ligated by the LiIII/XRCC1 complex.</p

53BP1 Damage Foci induction in human MRC5 fibroblasts.

<p>Images of unstressed (A) and stressed (B) cells expressing the fusion protein AcGFP-53BP1c. Scale bar represent 10 µm. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055190#pone.0055190.s004" target="_blank">Video S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055190#pone.0055190.s005" target="_blank">Video S2</a> for examples foci formation and resolution over time in unstressed and stressed MRC5 fibroblasts respectively.</p

Foci Longevity of live MRC5 cells observed for 30 hours.

<p>Foci Longevity of live MRC5 cells observed for 30 hours.</p

Damage foci longevities in live cells and simulations.

<p>(A) Longevities of foci recorded in unstressed MRC5 cells and the unstressed D-NHEJ and B-NHEJ model simulations. (B)Longevities of foci recorded in unstressed MRC5 cells and the stressed D-NHEJ and B-NHEJ model simulations with ROS production increased 2.5 times. Simulated data shows no change other than an increase in the number of breaks produced. (C) Survival curves of short lived foci (8 hours and less) for resting and stressed MRC5 cells (dotted lines) and resting and stressed simulated data (solid lines).</p

Effects of Ku70/80 redox on NHEJ.

<p>(A) Increasing Ku70/80′s and DNA-PK’s dissociation from DNA in line with observations from the literature (15) results in a decrease in short lived foci similar to that of stressed live cell. (B)Survival curves of short lived Foci (8 hours and less) for resting and stressed MRC5 cells (dotted lines) and resting and stressed simulated data (solid lines). Stressed data was collected from the model with increased Ku70/80 dissociation from DNA DSBs.</p