13 research outputs found

    Fugitive emissions in Moravian-Silesian Region

    Get PDF
    Import 22/07/2015Predlo┼żen├í pr├íca sa zaober├í fugit├şvnymi emisiami na ├║zem├ş priemyselnej aglomer├ície Ostravska. Fugit├şvny prach predstavuje hlavn├║ ─Źas┼ą atmosf├ęrick├Żch aeros├│lov, zv├Ż┼íen├í pozornos┼ą je mu venovan├í kv├┤li v├Żznamn├Żm dopadom na zmenu kl├şmy, kvalitu ovzdu┼íia a zdravie ─żud├ş a ekosyst├ęmov. Hlavn├í ─Źas┼ą pr├íce je venovan├í ┼ít├║diu vertik├ílnej distrib├║cie PM1 vo v├Ż┼íke a┼ż 500 m n. m., ktor├í bola sledovan├í vo vybran├Żch lokalit├ích Ostravy v jarnom a letnom obdob├ş 2014, za pou┼żitia met├│dy merania bal├│nom. Pozornos┼ą bola venovan├í z├ívislosti koncentr├ície PM1 na v├Ż┼íke a meteorologick├Żch podmienkach. ─Äalej bolo zis┼ąovan├ę rozlo┼żenie organick├Żch l├ítok vo vertik├ílnych profiloch atmosf├ęry v najza┼ąa┼żenej┼í├şch miestach Ostravy pou┼żit├şm met├│dy Py-GC/MS a pomocou matematick├Żch met├│d boli identifikovan├ę pr├şspevky zdrojov zne─Źistenia.This thesis deals with the topic of fugitive emissions in the industrial agglomeration of Ostrava region. Fugitive dust is a major part of atmospheric aerosols, increased attention is given to it due to its significant impact on climate change, air quality and human health, and ecosystems. The main part is focused on the study of the vertical distribution of PM1 of up to 500 m a. s. l. which was monitored at selected locations during spring and summer seasons of 2014 using the balloon measuring method. Attention was given to influence of meteorological parameters on PM1 concentrations. Furthermore, distribution of organic matter in the vertical profiles of the atmosphere in the most exposed places was studied using the Py-GC/MS and, using the mathematical methods, contributions of the sources of pollution were identified.Prezen─Źn├ş546 - Institut environment├íln├şho in┼żen├Żrstv├şv├Żborn

    1N3R-tau induces S phase arrest in HEK293 cells.

    No full text
    <p><b>(A)</b> The distribution of HEK293 with six tau isoforms in sub G1 phase. <b>(B)</b> The distribution of HEK293 with six tau isoforms in G1 phase. *<i>P</i> < 0.05, compared with vector. <b>(C)</b> The distribution of HEK293 with six tau isoforms in S phase. **<i>P</i> < 0.01, compared with vector. <b>(D)</b> The distribution of HEK293 with six tau isoforms in G2/M phase. *<i>P</i> < 0.05, compared with vector.</p

    1N3R-tau inhibits cell proliferation measured by BrdU incorporation.

    No full text
    <p>Fluorescent micrographs of eGFP and BrdU expression with six tau isoforms in N2a cells after 48 h transfection. GFP (Green), BrdU (Red). Quantification of BrdU-positive cells in eGFP expressing N2a cells with six tau isoforms after 48 h transfection. Scale bar = 50 ╬╝m. All values are standardized with vector. *<i>P</i> < 0.05, compared with vector.</p

    1N3R tau induces translocation of cyclin E from nucleus to cytoplasm.

    No full text
    <p><b>(A)</b> N2a cells were transfected transiently with full length tau (2N4R), 1N3R tau and empty vector for 48 h, then cytoplasm and nuclear proteins were extracted and cyclin A, cyclin B1, cyclin D1, cyclin E, Cdk2, Cdk4 were detected by western blotting. DM1A and Lamin B1 represented loading and nuclear reference respectively. <b>(B)</b> Quantitative analysis of (A). All values are standardized with vector. *<i>P</i> < 0.05, compared with vector.</p

    Intraperitoneal supplementation of magnesium rescues ICV-STZ-induced learning and memory deficits with elevation of brain magnesium level.

    No full text
    <p>The experiments were designed as shown in panel A. Rats were divided into six groups, i.e., sham-operated control (Con), sham-operated plus 100 mg/kg magnesium control (Mg), STZ ICV (STZ), STZ ICV plus 50 mg/kg magnesium (STZ+Mg50), STZ ICV plus 100 mg/kg magnesium (STZ+Mg100) and STZ ICV plus 200 mg/kg magnesium (STZ+Mg200) groups, as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108645#s2" target="_blank">Methods</a>. During the 3<sup>rd</sup> week after ICV-STZ treatment, the rats were trained in Morris watwe maze for six consecutive days to measure the learning capacity, and memory was tested on the 7<sup>th</sup> day via removal of the hidden platform. The escape latencies to find the hidden platform were recorded daily (B). For the memory test, the time spent in the target quadrant (C), the swimming tracks (D) and numbers of crossings (E) in the target quadrant were calculated. The rats were sacrificed after the behavioral tests, and the magnesium levels in the cerebral cortex (F) and hippocampus (G) were measured. Data were presented as means ┬▒ SD. *<i>P</i><0.05 versus the control group, <i>#P</i><0.05 versus the STZ group.</p

    Magnesium Protects Cognitive Functions and Synaptic Plasticity in Streptozotocin-Induced Sporadic AlzheimerÔÇÖs Model

    No full text
    <div><p>AlzheimerÔÇÖs disease (AD) is characterized by profound synapse loss and impairments of learning and memory. Magnesium affects many biochemical mechanisms that are vital for neuronal properties and synaptic plasticity. Recent studies have demonstrated that the serum and brain magnesium levels are decreased in AD patients; however, the exact role of magnesium in AD pathogenesis remains unclear. Here, we found that the intraperitoneal administration of magnesium sulfate increased the brain magnesium levels and protected learning and memory capacities in streptozotocin-induced sporadic AD model rats. We also found that magnesium sulfate reversed impairments in long-term potentiation (LTP), dendritic abnormalities, and the impaired recruitment of synaptic proteins. Magnesium sulfate treatment also decreased tau hyperphosphorylation by increasing the inhibitory phosphorylation of GSK-3╬▓ at serine 9, thereby increasing the activity of Akt at Ser473 and PI3K at Tyr458/199, and improving insulin sensitivity. We conclude that magnesium treatment protects cognitive function and synaptic plasticity by inhibiting GSK-3╬▓ in sporadic AD model rats, which suggests a potential role for magnesium in AD therapy.</p></div

    Magnesium suppresses GSK-3╬▓ with no effect on PP2A in the hippocampus of ICV-STZ-treated rats.

    No full text
    <p>Rats were divided into sham-operated control (Con), sham-operated plus 100 mg/kg magnesium control (Mg), STZ ICV (STZ) or STZ ICV plus 100 mg/kg magnesium (STZ+Mg) groups and treated as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108645#pone-0108645-g001" target="_blank">Figure 1A</a>. The total GSK-3╬▓, GSK-3╬▓ (Ser9), GSK-3╬▓ (Tyr216), total PP2A and PP2A (pY307) levels in whole hippocampus extracts were measured using Western blotting (A, C) and quantitative analysis (B, D). The level of p-GSK-3╬▓ (Ser9) was significantly decreased in ICV-STZ-treated rats, and magnesium supplementation restored the levels. No alterations in total GSK-3╬▓, p-GSK-3╬▓ (Y216), total PP2A and PP2A (pY307) levels were detected in these groups. Data were presented as means ┬▒ SD. *<i>P</i><0.05 versus the control group, <i>#P</i><0.05 versus the STZ group.</p

    Magnesium reverses ICV-STZ-induced dendritic spines and synapse impairments.

    No full text
    <p>Rats were divided into sham-operated control (Con), sham-operated plus 100 mg/kg magnesium control (Mg), STZ ICV (STZ) or STZ ICV plus 100 mg/kg magnesium (STZ+Mg) groups and treated as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108645#pone-0108645-g001" target="_blank">Figure 1A</a>. The representative morphology alterations of neurons in the hippocampal CA1 regions were displayed by Golgi staining. Representative photomicrographs of primary dendrites in the hippocampal CA1 region were shown (A). Quantification of dendrite number (B) and mushroom-type dendrites (C) were calculated. The number of dendritic branches and mushroom percentage in the hippocampus of ICV-STZ-treated rats decreased markedly, and supplement of magnesium almost fully reversed the dendritic complexity. Data were presented as means ┬▒ SD. *<i>P</i><0.05 versus the control group, <i>#P</i><0.05 versus the STZ group.</p

    Magnesium reverses the LTP deficiency induced by ICV-STZ.

    No full text
    <p>Rats were divided into sham-operated control (Con), sham-operated plus 100 mg/kg magnesium control (Mg), and STZ ICV (STZ) or STZ ICV plus 100 mg/kg magnesium (STZ+Mg) groups. During the 3<sup>rd</sup> week after ICV-STZ treatment, the hippocampal slices were prepared and an ideographic electrophysiology recording set-up with a stimulating electrode and recording electrode were placed in the CA3 and CA1 regions (A). The representative analog traces of evoked potentials before (solid line) and after (broken line) high-frequency stimulation (HFS) were recorded (B). Normalized field excitatory postsynaptic potential (fEPSP) slopes were measured in four groups (C), and the relative ratio of fEPSP increments after HFS (D) was calculated. Data were presented as means ┬▒ SD. *<i>P</i><0.05 versus the control group, <i>#P</i><0.05 versus the STZ group.</p
    corecore