Peripheral nerve-derived VEGF promotes arterial differentiation via neuropilin 1-mediated positive feedback

In developing limb skin, peripheral nerves are required for arterial differentiation, and guide the pattern of arterial branching. In vitro experiments suggest that nerve-derived VEGF may be important for arteriogenesis, but its role in vivo remains unclear. Using a series of nerve-specific Cre lines, we show that VEGF derived from sensory neurons, motoneurons and/or Schwann cells is required for arteriogenesis in vivo. Arteriogenesis also requires endothelial expression of NRP1, an artery-specific coreceptor for VEGF^(164) that is itself induced by VEGF. Our results provide the first evidence that VEGF is necessary for arteriogenesis from a primitive capillary plexus in vivo, and show that in limb skin the nerve is indeed the principal source of this signal. They also suggest a model in which a `winner-takes-all' competition for VEGF may control arterial differentiation, with the outcome biased by a VEGF^(164)-NRP1 positive-feedback loop. Our results also demonstrate that nerve-vessel alignment is a necessary, but not sufficient, condition for nerve-induced arteriogenesis. Different mechanisms therefore probably underlie these endothelial patterning and differentiation processes

Do Perceptions of Ballot Secrecy Influence Turnout? Results from a Field Experiment

Although the secret ballot has long been secured as a legal matter in the United States, formal secrecy protections are not equivalent to convincing citizens that they may vote privately and without fear of reprisal. We present survey evidence that those who have not previously voted are particularly likely to voice doubts about the secrecy of the voting process. We then report results from a field experiment where we provided registered voters with information about ballot secrecy protections prior to the 2010 general election. We find that these letters increased turnout for registered citizens without records of previous turnout, but did not appear to influence the behavior of citizens who had previously voted. These results suggest that although the secret ballot is a long-standing institution in the United States, providing basic information about ballot secrecy can affect the decision to participate to an important degree.

A multi-agent systems for design simulation framework:experiments with virtual-physical-social feedback for architecture

This paper presents research on the development of multiagent systems (MAS) for integrated and performance driven architectural design. It presents the development of a simulation framework that bridges architecture and engineering, through a series of multi-agent based experiments. The research is motivated to combine multiple design agencies into a system for managing and optimizing architectural form, across multiple objectives and contexts. The research anticipates the incorporation of feedback from real world human behavior and user preferences with physics based structural form finding and environmental analysis data. The framework is a multi-agent system that provides design teams with informed design solutions, which simultaneously optimize and satisfy competing design objectives. The initial results for building structures are measured in terms of the level of lighting improvements and qualitatively in geometric terms. Critical to the research is the elaboration of the system and the feedback loops that are possible when using the multi-agent systems approach