Diagrams for the statistical analysis on the <i>Pou5f1</i>-marked ES colony image set.

<p>Distribution related to the distances from the centroid compared to the null case distribution, with a statistical difference <i>p-value</i> of with significance level .</p

Segmentation process.

<p>(a): original image, (b): background subtraction. (c): colony segmentation, (d, e): orientation matching. (f): output image. Segmentation, identification and other outputs are shown overimposed on the original image (for a description see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080776#pone-0080776-g003" target="_blank">Figure 3</a>).</p

Diagrams for the statistical analysis on the <i>EndoA</i>-marked cell mutual distances.

<p>Distribution related to the mutual distances compared to the null case distribution, with a <i>p-value</i> of with significance level .</p

Process flow diagram for the proposed approach.

<p>A preprocessing step is used to remove the background and uniform the light intensity conditions, then the segmentation process takes place with a two-dimensional version of the Enhanced Interaction Model. The resulting binary image is then enhanced through a cascade of morphological operators. Different colonies are then processed through a Watershed transform which returns as output the segmentation for each colony in the image.</p

Diagrams for the statistical analysis on the <i>EndoA</i>-marked ES colony image set.

<p>Distribution related to the distances from the centroid compared to the null case distribution, with a statistical difference <i>p-value</i> of with significance level .</p

Segmentation examples.

<p>(a, b, c): original images. (d, e, f): segmented colonies and identified spotted cells. Colonies are segmented with different colors: in each one the central blue star point is the centroid while small green circles identify marked cells; the segment from the centroid to the marked cell defines the normalization path, whose value is shown next to the small circle.</p

Table of the parameters adopted for the analysis.

<p>Table of the parameters adopted for the analysis.</p

Diagrams for the statistical analysis on the <i>Zscan4</i>-marked cells mutual distances.

<p>Distribution related to the mutual distances compared to the null case distribution, with a <i>p-value</i> of with significance level .</p

Diagrams for the statistical analysis on the <i>Nanog</i>-marked ES colony image set.

<p>Distribution related to the distances from the centroid compared to the null case distribution, with a statistical difference <i>p-value</i> of with significance level .</p

Ablation of Gm12794 expressing ESCs.

<p>A) Schematic diagram of the p<i>Gm12794</i>-Strawberry reporter vector. The reporter gene <i>Strawberry</i> is placed under the control of a 5.0 kb region upstream the <i>Gm12794</i> ATG start codon. A PGK-Neo^r cassette is used for the selection of the ESC clones; the <i>Gm12794</i>-Strawberry reporter electroporated in mESCs is visualized by the Strawberry reporter gene (20x). B) Schematic diagram of the <i>Gm12794</i>-HSV-Tk vector electroporated in mESCs to generate ESC<i>^{Gm12794_HSVTK}</i> lines. ESC<i>^{Gm12794_HSVTK}</i> lines and control ESC<i>^HSVTK</i> lines were cultured in presence or absence of Ganciclovir (GCV) (2.0 µM, Sigma) (20x).</p