Forest plot of the relationship between miR-101 and OS in solid tumor.

<p>Forest plot of the relationship between miR-101 and OS in solid tumor.</p

Prognostic significance of microRNA-101 in solid tumor: A meta-analysis

<div><p>MicroRNA-101 has been reported as an important factor in carcinogenesis of several malignant tumors. However, its actual role in prognosis among solid malignancies remains unclear. Accordingly, we performed this meta-analysis aiming to identify prognostic significance of miR-101 in solid tumor. Pooled hazard ratios (HRs) with 95% confidence intervals (CIs) for overall survival (OS) or disease-free survival (DFS)/metastasis-free survival (MFS)/progression-free survival (PFS)/relapse-free survival (RFS)/time-to progression (TTP) were estimated with random effects or fixed effects models on the basis of heterogeneity. Subgroup analysis, sensitive analysis and meta-regression analysis were also conducted to clarify the possible confounding factors and investigate the source of heterogeneity. Publication bias was evaluated by using Begg’s and Egger’s tests. A total of 21 studies containing 3753 cases were selected into our quantitative analysis via electronic database search. A lower expression of miR-101 was significantly associated with worse OS (HR = 0.66, 95%CI [0.52–0.85], P = 0.001) and PFS (HR = 0.70, 95%CI [0.51–0.95], P = 0.023) in patients with solid tumor. The under-expression of miRNA-101 is a credible indicator of poorer prognosis in several of solid malignancies.</p></div

Flow diagram shows search strategy.

<p>Flow diagram shows search strategy.</p

Sensitivity analysis of the evaluation on the relationship between miR-101 and OS.

<p>Sensitivity analysis of the evaluation on the relationship between miR-101 and OS.</p

Characteristics of the included articles.

<p>Characteristics of the included articles.</p

Pooled HRs for OS according to subgroup analysis.

<p>Pooled HRs for OS according to subgroup analysis.</p

Funnel plots to evaluate publication bias of included studies for OS.

<p>Funnel plots to evaluate publication bias of included studies for OS.</p

Cytometric analysis of AGS cells after HP infection and/or IFN-γ treatment.

<p>B7-H1 expression in AGS cells was determined by flow cytometry after co-culturing the cells with HP (HP/cell ratio: 100/1) and/or treating the cells with IFN-γ (10 ng/ml) for 24 h. (A) Quantification of the cytometric data shows the percentages of B7-H1-positive AGS cells. (B) The ratio of the MFI (mean fluorescence intensity) of each group to the MFI of the isotype group.</p

Levels of miR-152 and miR-200b in gastric cancer cells after HP infection or IFN-γ treatment.

<p>AGS cells were co-cultured with HP (HP/cell ratio: 100/1) and/or treated with IFN-γ (100 ng/ml) for 24 h, followed by qPCR to measure the levels of miR-152 (A) and miR-200b (B) in cell lysates.</p

B7-H1 positive rate in clinical samples.

<p>B7-H1 positive rate in clinical samples.</p