Reactions of (-)-sparteine with alkali metal HMDS complexes : conventional meets the unconventional

Conventional (-)-sparteine adducts of lithium and sodium 1,1,1,3,3,3-hexamethyldisilazide (HMDS) were prepared and characterised, along with an unexpected and unconventional hydroxyl-incorporated sodium sodiate, [(-)-sparteine·Na(-HMDS)Na·(-)-sparteine]+[Na4(-HMDS)4(OH)]--the complex anion of which is the first inverse crown ether anion

Fire, boycott, threat and harm: social and political violence within the local community. A study of three Munster counties during the Irish Civil War, 1922-23.

In its investigation of social and political violence during the Irish Civil War, this thesis tackles the diverse range of deliberate, frightening and harmful actions—largely neglected by military and political histories of the conflict—that surfaced in local communities in Ireland during 1922–23. Through a three-county study of Limerick, Tipperary and Waterford, in the province of Munster, this thesis examines and explains violence perpetrated alongside and away from armed encounters between the anti-Treaty republican army and Free State forces. It identifies three main categories of violence: arson (the burning of houses, crops and infrastructure), intimidation (including boycott, damage to property, verbal and written threats, animal maiming, cattle driving and land seizure) and violence against the person (bodily damage or death through physical contact or the use of weapons). The thesis charts, where possible, the frequency of the violent act and, in exploring the symbolism and strategies involved in arson, intimidation and violence against the person, identifies two key functions of social and political violence. For one, targeted violence was used, during the Irish Civil War, to regulate community relations: state-sanctioned ethnic cleansing did not take place, but the religious and political minority (Protestants, ex-Servicemen and other British Loyalists) were deliberately persecuted, resulting in their flight from Munster. Land is another powerful motif in the thesis; the second key function of violence was to challenge attitudes towards rural issues and force redistribution outside the official channels. The thesis also places the Irish Civil War in perspective: the prolific bloodshed, sexual violence and gruesome torture witnessed in Central Europe, after World War I, did not become the norm in Ireland. Animals and private property bore the brunt of the severest actions in the three Munster counties. By bringing to light victims’ experiences of violence recorded in largely unexplored compensation claims, this thesis captures the complex questions of loyalty and identity—facing armed actors and officials, as well as civilians—that beset the violent and chaotic establishment of independent Ireland

An intersectionality-based policy analysis framework: critical reflections on a methodology for advancing equity

INTRODUCTION: In the field of health, numerous frameworks have emerged that advance understandings of the differential impacts of health policies to produce inclusive and socially just health outcomes. In this paper, we present the development of an important contribution to these efforts - an Intersectionality-Based Policy Analysis (IBPA) Framework. METHODS: Developed over the course of two years in consultation with key stakeholders and drawing on best and promising practices of other equity-informed approaches, this participatory and iterative IBPA Framework provides guidance and direction for researchers, civil society, public health professionals and policy actors seeking to address the challenges of health inequities across diverse populations. Importantly, we present the application of the IBPA Framework in seven priority health-related policy case studies. RESULTS: The analysis of each case study is focused on explaining how IBPA: 1) provides an innovative structure for critical policy analysis; 2) captures the different dimensions of policy contexts including history, politics, everyday lived experiences, diverse knowledges and intersecting social locations; and 3) generates transformative insights, knowledge, policy solutions and actions that cannot be gleaned from other equity-focused policy frameworks. CONCLUSION: The aim of this paper is to inspire a range of policy actors to recognize the potential of IBPA to foreground the complex contexts of health and social problems, and ultimately to transform how policy analysis is undertaken

Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli

Extraintestinal pathogenic E. coli (ExPEC) are the major aetiological agent of urinary tract infections (UTIs) in humans. The emergence of the CTX-M producing clone E. coli ST131 represents a major challenge to public health worldwide. A recent study on the metabolic potential of E. coli isolates demonstrated an association between the E. coli ST131 clone and enhanced utilisation of a panel of metabolic substrates. The studies presented here investigated the metabolic potential of ST131 and other major ExPEC ST isolates using 120 API test reagents and found that ST131 isolates demonstrated a lower metabolic activity for 5 of 120 biochemical tests in comparison to non-ST131 ExPEC isolates. Furthermore, comparative phenotypic microarray analysis showed a lack of specific metabolic profile for ST131 isolates countering the suggestion that these bacteria are metabolically fitter and therefore more successful human pathogens

Achieving high-sensitivity for clinical applications using augmented exome sequencing

daSNVs in ACMG genes where inadequate coverage was observed among at least one platform, using WES/ACE data normalized to both 12 Gb and 100× mean coverage. (XLSX 312 kb

Intersection between metabolic dysfunction, high fat diet consumption, and brain aging

Deleterious neurochemical, structural, and behavioral alterations are a seemingly unavoidable aspect of brain aging. However, the basis for these alterations, as well as the basis for the tremendous variability in regards to the degree to which these aspects are altered in aging individuals, remains to be elucidated. An increasing number of individuals regularly consume a diet high in fat, with high‐fat diet consumption known to be sufficient to promote metabolic dysfunction, although the links between high‐fat diet consumption and aging are only now beginning to be elucidated. In this review we discuss the potential role for age‐related metabolic disturbances serving as an important basis for deleterious perturbations in the aging brain. These data not only have important implications for understanding the basis of brain aging, but also may be important to the development of therapeutic interventions which promote successful brain aging.Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Bruce Keller, Annadora J.. State University of Louisiana; Estados UnidosFil: Morrison, Christopher D.. State University of Louisiana; Estados UnidosFil: Fernandez Kim, Sun Ok. State University of Louisiana; Estados UnidosFil: Ebenezer, Philip J.. State University of Louisiana; Estados UnidosFil: Zhang, Le. State University of Louisiana; Estados UnidosFil: Dasuri, Kalavathi. State University of Louisiana; Estados UnidosFil: Keller, Jeffrey N.. State University of Louisiana; Estados Unido

Strongly magnetized pulsars: explosive events and evolution

Well before the radio discovery of pulsars offered the first observational confirmation for their existence (Hewish et al., 1968), it had been suggested that neutron stars might be endowed with very strong magnetic fields of $10^{10}$-$10^{14}$G (Hoyle et al., 1964; Pacini, 1967). It is because of their magnetic fields that these otherwise small ed inert, cooling dead stars emit radio pulses and shine in various part of the electromagnetic spectrum. But the presence of a strong magnetic field has more subtle and sometimes dramatic consequences: In the last decades of observations indeed, evidence mounted that it is likely the magnetic field that makes of an isolated neutron star what it is among the different observational manifestations in which they come. The contribution of the magnetic field to the energy budget of the neutron star can be comparable or even exceed the available kinetic energy. The most magnetised neutron stars in particular, the magnetars, exhibit an amazing assortment of explosive events, underlining the importance of their magnetic field in their lives. In this chapter we review the recent observational and theoretical achievements, which not only confirmed the importance of the magnetic field in the evolution of neutron stars, but also provide a promising unification scheme for the different observational manifestations in which they appear. We focus on the role of their magnetic field as an energy source behind their persistent emission, but also its critical role in explosive events.Comment: Review commissioned for publication in the White Book of "NewCompStar" European COST Action MP1304, 43 pages, 8 figure

Communities of practice to facilitate change in health professions education: A realist synthesis

Background: Communities of practice could contribute to transformations in health professions education to meet complex and emerging challenges. However, little is known about the underlying mechanisms of communities of practice in this setting, and how context influences outcomes. Objective: To understand when, why and how communities of practice with health professions education faculty work to facilitate higher education change. Design: A realist synthesis according to the RAMESES standards and steps described by Pawson and colleagues. Review methods: Early scoping of the literature informed the development of an initial program theory to describe underlying assumptions about how communities of practice in higher education, implemented with health professions education faculty, were likely to work. The theory was tested and further refined through a realist synthesis. A systematic search for evidence using search terms ‘faculty’, ‘communities of practice’ and ‘higher education’ and related terms was supplemented with citation tracking and hand searching of significant authors and journals. Following study appraisal, data were extracted and synthesised from 21 manuscripts describing 16 communities of practice. The realist synthesis focused on identifying patterns in context-mechanism-outcome interactions, and the alignment with substantive theory. Results: From the included manuscripts, ten context-mechanism-outcome configurations were identified that describe a range of individual, interpersonal and institutional outcomes of communities of practice with health professions education faculty and context-mechanism interactions that contribute to achieving these outcomes. Conclusions: This study expands theoretical understandings of how and why communities of practice work. There is value in communities of practice in the higher education sector, primarily in the field of health professions education. Communities of practice implemented in the context of complex change with participants who have a desire to participate can facilitate change in health professions education, including institutional level changes, through reflection, experiential learning and creating a shared agenda for change. Findings from this study can be used by policy and decision-makers within health education to best apply communities of practice to achieve meaningful outcomes

Quantifying local rainfall dynamics and uncertain boundary conditions into a nested regional-local flood modeling system

[Abstract:] Inflow discharge and outflow stage estimates for hydraulic flood models are generally derived from river gauge data. Uncertainties in the measured inflow data and the neglect of rainfall-runoff contributions to the modeled domain downstream of the gauging locations can have a significant impact on these estimated “whole reach” inflows and consequently on flood predictions. In this study, a method to incorporate rating curve uncertainty and local rainfall-runoff dynamics into the predictions of a reach-scale flood model is proposed. The methodology is applied to the July 2007 floods of the River Severn in UK. Discharge uncertainty bounds are generated applying a nonparametric local weighted regression approach to stage-discharge measurements for two gauging stations. Measured rainfall downstream from these locations is used as input to a series of subcatchment regional hydrological model to quantify additional local inflows along the main channel. A regional simplified-physics hydraulic model is then applied to combine these contributions and generate an ensemble of discharge and water elevation time series at the boundaries of a local-scale high complexity hydraulic model. Finally, the effect of these rainfall dynamics and uncertain boundary conditions are evaluated on the local-scale model. Accurate prediction of the flood peak was obtained with the proposed method, which was only possible by resolving the additional complexity of the extreme rainfall contributions over the modeled area. The findings highlight the importance of estimating boundary condition uncertainty and local rainfall contributions for accurate prediction of river flows and inundation at regional scales.María Bermúdez gratefully acknowledges financial support from the Spanish Regional Government of Galicia (postdoctoral grant reference ED481B 2014/156-0). Gemma Coxon was supported by NERC MaRIUS: Managing the Risks, Impacts and Uncertainties of droughts and water Scarcity, grant NE/L010399/1. Jim Freer and Paul Bates by NERC Susceptibility of catchments to INTense RAinfall and flooding, grant NE/K00882X/1. A free version of the model LISFLOOD-FP is available for download at www.bristol.ac.uk/geography/research/hydrology/models/lisflood/. A free version of the model Iber is available for download at www.iberaula.es. The river cross-section data, the LiDAR digital elevation model and the gauging station rainfall, stage, flow and rating curve data of the presented case study are freely available from the Environment Agency ([email protected])Xunta de Galicia; ED481B 2014/156-0Inglaterra. University of Bristol; NE/L010399/1Inglaterra. University of Bristol; NE/K00882X/

Developing an understanding of canine adoption and how owner experience impacts on success

In 2010 in the United Kingdom an estimated 89,571 dogs passed through re-homing centres (Stavisky et al. 2012). Around 50% of these were relinquished by owners, with the rest largely being strays. Reasons for relinquishment have been widely reported. After adoption, the percentage of dogs being returned to re-homing centres (re-relinquishment) has been reported between 0% and 26% across a cross section of United Kingdom organisations (King, 2010). To date, research considering the success of canine adoption has been mostly retrospective and relatively limited in scope. The aim of this study was to investigate owner’s experience of adoption as they are the decision makers who ultimately control the outcome. This study recruited a prospective cohort of 248 dog owners and considered their aims and expectations of dog ownership at the pre-adoption stage. 112 particpants shared their experience post adoption. A mixed methods approach was used integrating both qualitative and quantitative research. Seven different re-homing organisations were recruited to encompass a range of different re-homing policies. The study identified that most owners have previous experience of dog adoption, and that companionship is a key motivation for ownership. Most owners choose to source dogs from a rehoming centre for altruistic reasons. Owner expectations of dog ownership were mostly positive. Owners frequently reported problems in the post adoption period however they still reported that they perceived that dogs behaved significantly better than expected when they first went home. Participants showed reluctance to return dogs although they were more likely to consider this option in the event of their dog being aggressive. The overall re-relinquishment rate for all organisations was 12% but ranged from 4% to 20% This study adds greater depth to our understanding of how owners experience canine adoption. Opportunities for strengthening the human-animal bond are identified to promote successful re-homing