Relationship between rCsCP concentrations and OD values.

<p>Bovine serum slbumin (BSA) was used as a negative control. OD values of rCsCp are expressed as mean OD values measured with IgY-IMB-ELISA from two independent combined experiments.</p

Detection results in sera of human clonorchiasis with different egg per gram faces (EPG) by IgY-IMB-ELISA.

<p>Each sample had duplicate tubes and the experiment was repeated twice.</p><p>Detection results in sera of human clonorchiasis with different egg per gram faces (EPG) by IgY-IMB-ELISA.</p

Analysis of IgY by 12% SDS-PAGE in non-reducing condition.

<p>4 µg of protein was loaded for each lane. M: Protein molecular weight standards marker; 1: IgY from immunized egg yolk; 2: IgY from non-immunized egg yolk.</p

Analysis of IgY by Western blot.

<p>M: Protein molecular weight standards marker; 1: rCsCP probed with purified IgY from immunized egg yolk; 2: rCsCP probed with purified IgY from non-immunized egg yolk.</p

Comparison of OD values obtained in IgY-IMB-ELISA for detection of serum circulating antigens from clonorchiasis patients with different infection intensity (egg per gram of feces, EPG).

<p>Comparison of OD values obtained in IgY-IMB-ELISA for detection of serum circulating antigens from clonorchiasis patients with different infection intensity (egg per gram of feces, EPG).</p