Optimization for the production of a polyketone <i>3S,4S-</i>DMD from <i>Panus lecomtei</i> (Agaricomycetes) by submerged fermentation

3,4-Dihydroxy-2,2-dimethyl-chroman derivatives have diverse physiological properties. A polyketone (3S,4S)-3,4-Dihydroxy-6-methoxy-2,2-dimethylchromom (3S,4S-DMD) with antibacterial activity was isolated from the solid culture of rare edible fungus Panus lecomtei. However, the yield of 3S,4S-DMD in solid culture of P. lecomtei is very low and the production period are too long. In this work, efficient accumulation of 3S,4S-DMD in P. lecomtei by submerged fermentation is studied. The key fermentation factors of P. lecomtei for 3S,4S-DMD production were optimised by single-factor experiment successively, and then a Box-Behnken design (BBD) experiment was carried out to further enhance 3S,4S-DMD production. A maximum 3S,4S-DMD yield of 196.3 mg/L was obtained at 25.78 g/L glucose, 1.67 g/L MgSO4 · 7H2O, 40°C and 197 r/min, respectively, which increased by 1.3-fold in comparison with that in the non-optimised fermentation conditions. Furthermore, an enhanced yield of 3S,4S-DMD (261.6 mg/L) was obtained in 5-L agitated fermenter. The 3S,4S-DMD productivity in flask and fermenter reached to 7.26 and 8.07 mg/g per day, respectively, which considerably increased by over 121-fold in comparison with that in the solid fermentation (0.06 mg/g per day). This study presents a potential method for the production of 3S,4S-DMD by submerged fermentation.</p

Additional file 5 of Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

Additional file 5: Functional classification of identified proteins from secretome of T. hirsuta X-13

Additional file 1 of Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

Additional file 1: Figure S1. Detection of colorization and decolorization zone by T. hirsuta X-13 on guaiacol-containing (a and b) and Azure B-containing (c and d) PDA medium. A and C, control; B and D, inoculated with T. hirsuta X-13

Additional file 6 of Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

Additional file 6: Figure S3. Distribution of the identified proteins based on functional classification

Additional file 8 of Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

Additional file 8: Figure S4. The total ion chromatograms of the control (a) and lignin samples treated with T. hirsuta X-13 for 13 days (b)

Additional file 3 of Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

Additional file 3: Table S1. The percentage of main components in alkaline lignin

Additional file 9 of Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

Additional file 9: Table S3. The identified pyrolysates from lignin samples before and after treated by T. hirsuta X-13 at different time with relative peak areas

Additional file 1 of Biochemical and molecular characterization of a novel glycerol dehydratase from Klebsiella pneumoniae 2e with high tolerance against crude glycerol impurities

Additional file 1: Table S1. Primers used for PCR in this study

Additional file 4 of Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

Additional file 4: The identified proteins from secretome of T. hirsuta X-13 in lignin-containing medium