2 research outputs found
Π€ΡΡΠΎΡ ΡΠΌΡΡΠ½Π΅ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π»ΠΈΡΡΡ Salvia grandiflora ΡΠ° Salvia officinalis Π΄Π»Ρ Π²ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½Ρ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²ΠΈ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½Ρ Ρ ΠΌΠ΅Π΄ΠΈΡΠ½ΡΠΉ ΡΠ° ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ½ΡΠΉ ΠΏΡΠ°ΠΊΡΠΈΡΡ
The raw material base of medicinal plants in Ukraine is sufficient only for half of the pharmacopoeial species. Most part of the medicinal plants is growing in insufficient quantities and there is a need for their import. In conditions of import dependence and a shortage of domestic plant materials, the search for new sources of biologically active substances among the representatives of the flora of Ukraine is an urgent task of modern pharmaceutical science.The aim of the work β conduct a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis to establish the possibility of using the non-pharmacopeia species in pharmaceutical and medical practice.Materials and methods. The objects of the study are the leaves of S. grandiflora and S. officinalis, which was harvested in the botanical garden of the Lviv National University named after I. Franko. The study of macro- and microelement composition in the leaves of S. officinalis and S. grandiflora was carried out by atomic emission spectrographic method. Determination of the qualitative composition and quantitative content of the main groups of biologically active substances was carried out by HPLC. Quantitative determination of phenolic compounds was also carried out by spectrophotometric method.Results. The content of 15 micro and macro elements was found in both studied species. In the leaves of S. officinalis and S. grandiflora, 15 amino acids and 8 saponins were identified. Using HPLC, the qualitative composition and quantitative content of phenolic substances in the leaves of S. officinalis and S. grandiflora (13 and 9 compounds, respectively) was established.Discussion. The dominant macro and micro elements in the studied species were silicon, phosphorus, magnesium, calcium, sodium, and potassium. The total content of trace elements in the leaves of S. grandiflora is 1.67 times greater than in the pharmacopeia plant S. officinalis. The dominant amino acids in the leaves of both species are glutamic acid, aspartic acid, valine and leucine.The dominant saponins in the leaf of S. officinalis were ursolic and oleanolic acids, the total content of which is 75.82 %. In the leaves of S.grandiflora, ursolic and euskapic acids were dominant, with a total content of 63.25 %.The total flavonoid content is higher in S. officinalis leaf and is 4.90 mg / g. The total content of hydroxycinnamic acids is highest in the leaf of S. grandiflora and is 4.49 mg / g, which is 221.18 % (2.21 times) higher than in the pharmacopeia plant S. officinalis (2.03 mg / g). The total highest content of caffeic acid derivatives prevails in the Salvia officinalis leaf (0.77 mg / g). The highest content of the sum of all detected phenolic compounds is specified for S. officinalis leaves and amounts to 6.93 mg / g.Conclusions. As a result of a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis, it was established that S. grandiflora is a promising species for introduction into medical and pharmaceutical practice, namely, as a source of phenolic compoundsΠ‘ΡΡΡΠ΅Π²Π°Ρ Π±Π°Π·Π° Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ Π£ΠΊΡΠ°ΠΈΠ½Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΠΎΠ»ΡΠΊΠΎ Π΄Π»Ρ ΠΏΠΎΠ»ΠΎΠ²ΠΈΠ½Ρ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΡΡ
Π²ΠΈΠ΄ΠΎΠ². ΠΠΎΠ»ΡΡΠ°Ρ ΡΠ°ΡΡΡ ΠΠ Π‘ ΡΠ°ΡΡΠ΅Ρ Π² Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅ ΠΈ Π²ΠΎΠ·Π½ΠΈΠΊΠ°Π΅Ρ ΠΏΠΎΡΡΠ΅Π±Π½ΠΎΡΡΡ Π² ΠΈΡ
ΠΈΠΌΠΏΠΎΡΡΠ΅. Π ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΠΈΠΌΠΏΠΎΡΡΠΎΠ·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΈ Π΄Π΅ΡΠΈΡΠΈΡΠ° ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΡΡΡ, ΠΏΠΎΠΈΡΠΊ Π½ΠΎΠ²ΡΡ
ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠ² Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ
Π²Π΅ΡΠ΅ΡΡΠ² ΡΡΠ΅Π΄ΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ ΡΠ»ΠΎΡΡ Π£ΠΊΡΠ°ΠΈΠ½Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π°ΡΠΊΠΈ.Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ β ΠΏΡΠΎΠ²Π΅ΡΡΠΈ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΠΈΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π»ΠΈΡΡΡΠ΅Π² S. grandiflora ΠΈ S. officinalis Π΄Π»Ρ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π½Π΅ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΠΎΠ³ΠΎ Π²ΠΈΠ΄Π° Π² ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ±ΡΠ΅ΠΊΡΠ°ΠΌΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ²Π»ΡΠ΅ΡΡΡ Π»ΠΈΡΡΡΡ S.Β grandiflora ΠΈ S. officinalis, ΠΊΠΎΡΠΎΡΠΎΠ΅ Π±ΡΠ»ΠΎ Π·Π°Π³ΠΎΡΠΎΠ²Π»Π΅Π½ΠΎ Π² Π±ΠΎΡΠ°Π½ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΡΠ°Π΄Ρ ΠΡΠ²ΠΎΠ²ΡΠΊΠΎΠ³ΠΎ Π½Π°ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ½ΠΈΠ²Π΅ΡΡΠΈΡΠ΅ΡΠ° ΠΈΠΌΠ΅Π½ΠΈ Π. Π€ΡΠ°Π½ΠΊΠΎ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ°ΠΊΡΠΎ- ΠΈ ΠΌΠΈΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° Π² Π»ΠΈΡΡΡΡΡ
S. officinalis ΠΈ S. grandiflora ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π°ΡΠΎΠΌΠ½ΠΎ-ΡΠΌΠΈΡΡΠΈΠΎΠ½Π½ΡΠΌ ΡΠΏΠ΅ΠΊΡΡΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
Π³ΡΡΠΏΠΏ ΠΠΠ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠΠΠ₯. ΠΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π ΠΎΠ±Π΅ΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
Π²ΠΈΠ΄Π°Ρ
ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ 15 ΠΌΠΈΠΊΡΠΎ- ΠΈ ΠΌΠ°ΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ². Π Π»ΠΈΡΡΡΡΡ
S.Β officinalis ΠΈ S. grandiflora Π±ΡΠ»ΠΎ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΎ 15 Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡ ΠΈ 8 ΡΠ°ΠΏΠΎΠ½ΠΈΠ½ΠΎΠ². ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠΠΠ₯ Π±ΡΠ»ΠΎ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΉ ΡΠΎΡΡΠ°Π² ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π²Π΅ΡΠ΅ΡΡΠ² ΡΠ΅Π½ΠΎΠ»ΡΠ½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ Π² Π»ΠΈΡΡΡΡΡ
S.Β officinalis ΠΈ S. grandiflora (13 ΠΈ 9 ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ).ΠΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. ΠΠΎΠΌΠΈΠ½ΠΈΡΡΡΡΠΈΠΌΠΈ ΠΌΠ°ΠΊΡΠΎ- ΠΈ ΠΌΠΈΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΠΌΠΈ Π² ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
Π²ΠΈΠ΄Π°Ρ
Π±ΡΠ»ΠΈ ΠΊΡΠ΅ΠΌΠ½ΠΈΠΉ, ΡΠΎΡΡΠΎΡ, ΠΌΠ°Π³Π½ΠΈΠΉ, ΠΊΠ°Π»ΡΡΠΈΠΉ, Π½Π°ΡΡΠΈΠΉ ΠΈ ΠΊΠ°Π»ΠΈΠΉ. ΠΠ±ΡΠ΅Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΌΠΈΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² Π»ΠΈΡΡΡΡΡ
S. grandiflora Π² 1,67 ΡΠ°Π· Π±ΠΎΠ»ΡΡΠ΅, ΡΠ΅ΠΌ Π² ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΠΎΠΌ Π²ΠΈΠ΄Π΅ S. officinalis. ΠΠΎΠΌΠΈΠ½ΠΈΡΡΡΡΠΈΠΌΠΈ Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌΠΈ Π² Π»ΠΈΡΡΡΡΡ
ΠΎΠ±ΠΎΠΈΡ
Π²ΠΈΠ΄ΠΎΠ² ΡΠ²Π»ΡΠ΅ΡΡΡ Π³Π»ΡΡΠ°ΠΌΠΈΠ½ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ°, Π°ΡΠΏΠ°ΡΠ°Π³ΠΈΠ½ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ°, Π²Π°Π»ΠΈΠ½ ΠΈ Π»Π΅ΠΉΡΠΈΠ½.ΠΠΎΠΌΠΈΠ½ΠΈΡΡΡΡΠΈΠΌΠΈ ΡΠ°ΠΏΠΎΠ½ΠΈΠ½Π°ΠΌΠΈ Π² Π»ΠΈΡΡΠ΅ S. officinalis Π±ΡΠ»ΠΈ ΡΡΡΠΎΠ»ΠΎΠ²Π°Ρ ΠΈ ΠΎΠ»Π΅Π°Π½ΠΎΠ»ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΡ, ΠΎΠ±ΡΠ΅Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΊΠΎΡΠΎΡΡΡ
ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ 75,82 %. Π Π»ΠΈΡΡΠ΅ S.grandiflora Π΄ΠΎΠΌΠΈΠ½ΠΈΡΡΡΡΠΈΠΌΠΈ Π±ΡΠ»ΠΈ ΡΡΡΠΎΠ»ΠΎΠ²Π°Ρ ΠΈ Π΅ΡΡΠΊΠ°ΠΏΠΎΠ²Π° ΠΊΠΈΡΠ»ΠΎΡΡ, ΠΎΠ±ΡΠ΅Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΊΠΎΡΠΎΡΡΡ
ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ 63,25 %.ΠΠ±ΡΠ΅Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π±ΠΎΠ»ΡΡΠ΅ Π² Π»ΠΈΡΡΠ΅ S. officinalis ΠΈ ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ 4,90 ΠΌΠ³/Π³. ΠΠ±ΡΠ΅Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΊΠΎΡΠΈΡΠ½ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ΅Π΅ Π² Π»ΠΈΡΡΠ΅ S. grandiflora ΠΈ ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ 4,49 ΠΌΠ³/Π³, ΡΡΠΎ Π½Π° 221,18 % (Π² 2,21 ΡΠ°Π·Π°) Π±ΠΎΠ»ΡΡΠ΅ ΡΠ΅ΠΌ Π² ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΠΎΠΌ Π²ΠΈΠ΄Π΅ S. officinalis (2,03 ΠΌΠ³/Π³). ΠΠ±ΡΠ΅Π΅ Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ΅Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
ΠΊΠΎΡΠ΅ΠΉΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ Π² Π»ΠΈΡΡΠ΅ Salvia officinalis (0,77 ΠΌΠ³/Π³). ΠΠ°ΠΈΠ±ΠΎΠ»ΡΡΠ΅Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΡΠΌΠΌΡ Π²ΡΠ΅Ρ
Π²ΡΡΠ²Π»Π΅Π½Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΠΎ Π΄Π»Ρ Π»ΠΈΡΡΡΠ΅Π² S. officinalis ΠΈ ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ 6,93 ΠΌΠ³ / Π³.ΠΡΠ²ΠΎΠ΄Ρ. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠ³ΠΎ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π»ΠΈΡΡΡΠ΅Π² S. grandiflora ΠΈ S. officinalis ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ S. grandiflora ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ Π²ΠΈΠ΄ΠΎΠΌ Π΄Π»Ρ Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΡ Π² ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΡΡ ΠΈ ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΡΡ ΠΏΡΠ°ΠΊΡΠΈΠΊΡ, Π° ΠΈΠΌΠ΅Π½Π½ΠΎ ΠΊΠ°ΠΊ ΠΈΡΡΠΎΡΠ½ΠΈΠΊ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉΠ‘ΠΈΡΠΎΠ²ΠΈΠ½Π½Π° Π±Π°Π·Π° Π»ΡΠΊΠ°ΡΡΡΠΊΠΈΡ
ΡΠΎΡΠ»ΠΈΠ½ Π£ΠΊΡΠ°ΡΠ½ΠΈ Ρ Π΄ΠΎΡΡΠ°ΡΠ½ΡΠΎΡ ΡΡΠ»ΡΠΊΠΈ Π΄Π»Ρ ΠΏΠΎΠ»ΠΎΠ²ΠΈΠ½ΠΈ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΠΈΡ
Π²ΠΈΠ΄ΡΠ². ΠΠ΅Π»ΠΈΠΊΠ° ΡΠ°ΡΡΠΈΠ½Π° ΠΠ Π‘ Π·ΡΠΎΡΡΠ°Ρ Ρ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠ½ΡΠΉ ΠΊΡΠ»ΡΠΊΠΎΡΡΡ ΡΠ° Π²ΠΈΠ½ΠΈΠΊΠ°Ρ ΠΏΠΎΡΡΠ΅Π±Π° Π² ΡΡ
ΡΠΌΠΏΠΎΡΡΡΠ²Π°Π½Π½Ρ. Π ΡΠΌΠΎΠ²Π°Ρ
ΡΠΌΠΏΠΎΡΡΠΎΠ·Π°Π»Π΅ΠΆΠ½ΠΎΡΡΡ ΡΠ° Π΄Π΅ΡΡΡΠΈΡΡ Π²ΡΡΡΠΈΠ·Π½ΡΠ½ΠΎΡ ΡΠΎΡΠ»ΠΈΠ½Π½ΠΎΡ ΡΠΈΡΠΎΠ²ΠΈΠ½ΠΈ, ΠΏΠΎΡΡΠΊ Π½ΠΎΠ²ΠΈΡ
Π΄ΠΆΠ΅ΡΠ΅Π» Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎ Π°ΠΊΡΠΈΠ²Π½ΠΈΡ
ΡΠ΅ΡΠΎΠ²ΠΈΠ½ ΡΠ΅ΡΠ΅Π΄ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π½ΠΈΠΊΡΠ² ΡΠ»ΠΎΡΠΈ Π£ΠΊΡΠ°ΡΠ½ΠΈ Ρ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΈΠΌ Π·Π°Π²Π΄Π°Π½Π½ΡΠΌ ΡΡΡΠ°ΡΠ½ΠΎΡ ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ½ΠΎΡ Π½Π°ΡΠΊΠΈ.ΠΠ΅ΡΠ° ΡΠΎΠ±ΠΎΡΠΈ β ΠΏΡΠΎΠ²Π΅ΡΡΠΈ ΠΏΠΎΡΡΠ²Π½ΡΠ»ΡΠ½Π΅ ΡΡΡΠΎΡ
ΡΠΌΡΡΠ½Π΅ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π»ΠΈΡΡΡ S. grandiflora ΡΠ° S. officinalis Π΄Π»Ρ Π²ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½Ρ ΠΌΠΎΠΆΠ»ΠΈΠ²ΠΎΡΡΡ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½Ρ Π½Π΅ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΠΎΠ³ΠΎ Π²ΠΈΠ΄Ρ Ρ ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ½ΡΠΉ ΡΠ° ΠΌΠ΅Π΄ΠΈΡΠ½ΡΠΉ ΠΏΡΠ°ΠΊΡΠΈΡΡ.ΠΠ°ΡΠ΅ΡΡΠ°Π»ΠΈ ΡΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΈ. ΠΠ±βΡΠΊΡΠΎΠΌΠΈ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π±ΡΠ»ΠΎ Π»ΠΈΡΡΡ S. grandiflora ΡΠ° S. officinalis, ΡΠΊΠ΅ Π±ΡΠ»ΠΎ Π·Π°Π³ΠΎΡΠΎΠ²Π»Π΅Π½ΠΎ Ρ Π±ΠΎΡΠ°Π½ΡΡΠ½ΠΎΠΌΡ ΡΠ°Π΄Ρ ΠΡΠ²ΡΠ²ΡΡΠΊΠΎΠ³ΠΎ Π½Π°ΡΡΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ½ΡΠ²Π΅ΡΡΠΈΡΠ΅ΡΡ ΡΠΌΠ΅Π½Ρ Π. Π€ΡΠ°Π½ΠΊΠ°. ΠΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ ΠΌΠ°ΠΊΡΠΎ- ΡΠ° ΠΌΡΠΊΡΠΎΠ΅Π»Π΅ΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠ»Π°Π΄Ρ Ρ Π»ΠΈΡΡΡ S. officinalis ΡΠ° S. grandiflora ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π°ΡΠΎΠΌΠ½ΠΎ-Π΅ΠΌΡΡΡΠΉΠ½ΠΈΠΌ ΡΠΏΠ΅ΠΊΡΡΠΎΠ³ΡΠ°ΡΡΡΠ½ΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ. ΠΠΈΠ·Π½Π°ΡΠ΅Π½Π½Ρ ΡΠΊΡΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠ»Π°Π΄Ρ ΡΠ° ΠΊΡΠ»ΡΠΊΡΡΠ½ΠΎΠ³ΠΎ Π²ΠΌΡΡΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΠΈΡ
Π³ΡΡΠΏ ΠΠΠ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠΠ Π₯. ΠΡΠ»ΡΠΊΡΡΠ½Π΅ Π²ΠΈΠ·Π½Π°ΡΠ΅Π½Π½Ρ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ ΡΠ°ΠΊΠΎΠΆ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ½ΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΠΈ. Π ΠΎΠ±ΠΎΡ
Π΄ΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π½ΠΈΡ
Π²ΠΈΠ΄Π°Ρ
Π²ΠΈΡΠ²Π»Π΅Π½ΠΎ Π²ΠΌΡΡΡ 15 ΠΌΡΠΊΡΠΎ- ΡΠ° ΠΌΠ°ΠΊΡΠΎΠ΅Π»Π΅ΠΌΠ΅Π½ΡΡΠ². Π£ Π»ΠΈΡΡΡ S. officinalis ΡΠ° S. grandiflora Π±ΡΠ»ΠΎ ΡΠ΄Π΅Π½ΡΠΈΡΡΠΊΠΎΠ²Π°Π½ΠΎ 15 Π°ΠΌΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡ ΡΠ° 8 ΡΠ°ΠΏΠΎΠ½ΡΠ½ΡΠ². ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠΠ Π₯ Π±ΡΠ»ΠΎ Π²ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΡΠΊΡΡΠ½ΠΈΠΉ ΡΠΊΠ»Π°Π΄ ΡΠ° ΠΊΡΠ»ΡΠΊΡΡΠ½ΠΈΠΉ Π²ΠΌΡΡΡ ΡΠ΅ΡΠΎΠ²ΠΈΠ½ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΠΎΡ ΠΏΡΠΈΡΠΎΠ΄ΠΈ Ρ Π»ΠΈΡΡΡ S. officinalis ΡΠ° S. grandiflora (13 ΡΠ° 9 ΡΠΏΠΎΠ»ΡΠΊ Π²ΡΠ΄ΠΏΠΎΠ²ΡΠ΄Π½ΠΎ).ΠΠ±Π³ΠΎΠ²ΠΎΡΠ΅Π½Π½Ρ. ΠΠΎΠΌΡΠ½ΡΡΡΠΈΠΌΠΈ ΠΌΡΠΊΡΠΎ- ΡΠ° ΠΌΠ°ΠΊΡΠΎΠ΅Π»Π΅ΠΌΠ΅Π½ΡΡΠ² Ρ ΠΎΠ±ΠΎΡ
Π²ΠΈΠ΄Π°Ρ
ΡΠΈΡΠΎΠ²ΠΈΠ½ΠΈ Π±ΡΠ»ΠΈ ΡΠΈΠ»ΡΡΡΠΉ, ΡΠΎΡΡΠΎΡ, ΠΌΠ°Π³Π½ΡΠΉ, ΠΊΠ°Π»ΡΡΡΠΉ, Π½Π°ΡΡΡΠΉ ΡΠ° ΠΊΠ°Π»ΡΠΉ. ΠΠ°Π³Π°Π»ΡΠ½ΠΈΠΉ Π²ΠΌΡΡΡ ΠΌΡΠΊΡΠΎΠ΅Π»Π΅ΠΌΠ΅Π½ΡΡΠ² Ρ Π»ΠΈΡΡΡ S. grandiflora Ρ 1,67 ΡΠ°Π· Π±ΡΠ»ΡΡΠΈΠΉ Π½ΡΠΆ Ρ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΠΎΠΌΡ Π²ΠΈΠ΄Ρ S.Β officinalis. ΠΠΎΠΌΡΠ½ΡΡΡΠΈΠΌΠΈ Π°ΠΌΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌΠΈ Π² Π»ΠΈΡΡΡ ΠΎΠ±ΠΎΡ
Π²ΠΈΠ΄ΡΠ² Ρ Π³Π»ΡΡΠ°ΠΌΡΠ½ΠΎΠ²Π° ΠΊΠΈΡΠ»ΠΎΡΠ°, Π°ΡΠΏΠ°ΡΠ°Π³ΡΠ½ΠΎΠ²Π° ΠΊΠΈΡΠ»ΠΎΡΠ°, Π²Π°Π»ΡΠ½ ΡΠ° Π»Π΅ΠΉΡΠΈΠ½.ΠΠΎΠΌΡΠ½ΡΡΡΠΈΠΌΠΈ ΡΠ°ΠΏΠΎΠ½ΡΠ½Π°ΠΌΠΈ Ρ Π»ΠΈΡΡΡ S. officinalis Π±ΡΠ»ΠΈ ΡΡΡΠΎΠ»ΠΎΠ²Π° ΡΠ° ΠΎΠ»Π΅Π°Π½ΠΎΠ»ΠΎΠ²Π° ΠΊΠΈΡΠ»ΠΎΡΠΈ, Π·Π°Π³Π°Π»ΡΠ½ΠΈΠΉ Π²ΠΌΡΡΡ ΡΠΊΠΈΡ
ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ 75,82 %. Π£ Π»ΠΈΡΡΡ S.grandiflora Π΄ΠΎΠΌΡΠ½ΡΡΡΠΈΠΌΠΈ Π±ΡΠ»ΠΈ ΡΡΡΠΎΠ»ΠΎΠ²Π° ΡΠ° Π΅ΡΡΠΊΠ°ΠΏΠΎΠ²Π° ΠΊΠΈΡΠ»ΠΎΡΠΈ, Π·Π°Π³Π°Π»ΡΠ½ΠΈΠΉ Π²ΠΌΡΡΡ ΡΠΊΠΈΡ
ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ 63,25 %.ΠΠ°Π³Π°Π»ΡΠ½ΠΈΠΉ Π²ΠΌΡΡΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΡΠ΄ΡΠ² Π½Π°ΠΉΠ±ΡΠ»ΡΡΠΈΠΉ Π² Π»ΠΈΡΡΡ S. officinalis ΡΠ° ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ 4,90 ΠΌΠ³/Π³. ΠΠ°Π³Π°Π»ΡΠ½ΠΈΠΉ Π²ΠΌΡΡΡ Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΊΠΎΡΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Π½Π°ΠΉΠ±ΡΠ»ΡΡΠΈΠΉ Π² Π»ΠΈΡΡΡ S. grandiflora ΡΠ° ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ 4,49 ΠΌΠ³/Π³, ΡΠΎ Π½Π° 221,18 % (Π² 2,21 ΡΠ°Π·ΠΈ) Π±ΡΠ»ΡΡΠ΅ Π½ΡΠΆ Ρ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΠΎΠΌΡ Π²ΠΈΠ΄Ρ S. officinalis (2,03ΠΌΠ³/Π³). ΠΠ°Π³Π°Π»ΡΠ½ΠΈΠΉ Π½Π°ΠΉΠ±ΡΠ»ΡΡΠΈΠΉ Π²ΠΌΡΡΡ ΠΏΠΎΡ
ΡΠ΄Π½ΠΈΡ
ΠΊΠ°Π²ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ ΠΏΠ΅ΡΠ΅Π²Π°ΠΆΠ°Ρ Π² Π»ΠΈΡΡΡ Salvia officinalis (0,77 ΠΌΠ³/Π³). ΠΠ°ΠΉΠ±ΡΠ»ΡΡΠΈΠΉ Π²ΠΌΡΡΡ ΡΡΠΌΠΈ Π²ΡΡΡ
Π²ΠΈΡΠ²Π»Π΅Π½ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΠΎΡ ΠΏΡΠΈΡΠΎΠ΄ΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΠΈΠΉ Π΄Π»Ρ Π»ΠΈΡΡΡ S. officinalis ΡΠ° ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ 6,93 ΠΌΠ³/Π³.ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. Π£ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΡΠ²Π½ΡΠ»ΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠΎΡ
ΡΠΌΡΡΠ½ΠΎΠ³ΠΎ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π»ΠΈΡΡΡ S. grandiflora ΡΠ° S. officinalis Π²ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΠΎ S. grandiflora Ρ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΈΠΌ Π²ΠΈΠ΄ΠΎΠΌ Π΄Π»Ρ Π²ΠΏΡΠΎΠ²Π°Π΄ΠΆΠ΅Π½Π½Ρ Ρ ΠΌΠ΅Π΄ΠΈΡΠ½Ρ ΡΠ° ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ½Ρ ΠΏΡΠ°ΠΊΡΠΈΠΊΡ ΡΠ°ΠΌΠ΅ ΡΠΊ Π΄ΠΆΠ΅ΡΠ΅Π»ΠΎ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΠΈΡ
ΡΠΏΠΎΠ»Ρ
Phytochemical Study of Salvia Grandiflora and Salvia Officinalis Leaves for Establishing Prospects for Use in Medical and Pharmaceutical Practice
The raw material base of medicinal plants in Ukraine is sufficient only for half of the pharmacopoeial species. Most part of the medicinal plants is growing in insufficient quantities and there is a need for their import. In conditions of import dependence and a shortage of domestic plant materials, the search for new sources of biologically active substances among the representatives of the flora of Ukraine is an urgent task of modern pharmaceutical science.The aim of the work β conduct a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis to establish the possibility of using the non-pharmacopeia species in pharmaceutical and medical practice.Materials and methods. The objects of the study are the leaves of S. grandiflora and S. officinalis, which was harvested in the botanical garden of the Lviv National University named after I. Franko. The study of macro- and microelement composition in the leaves of S. officinalis and S. grandiflora was carried out by atomic emission spectrographic method. Determination of the qualitative composition and quantitative content of the main groups of biologically active substances was carried out by HPLC. Quantitative determination of phenolic compounds was also carried out by spectrophotometric method.Results. The content of 15 micro and macro elements was found in both studied species. In the leaves of S. officinalis and S. grandiflora, 15 amino acids and 8 saponins were identified. Using HPLC, the qualitative composition and quantitative content of phenolic substances in the leaves of S. officinalis and S. grandiflora (13 and 9 compounds, respectively) was established.Discussion. The dominant macro and micro elements in the studied species were silicon, phosphorus, magnesium, calcium, sodium, and potassium. The total content of trace elements in the leaves of S. grandiflora is 1.67 times greater than in the pharmacopeia plant S. officinalis. The dominant amino acids in the leaves of both species are glutamic acid, aspartic acid, valine and leucine.The dominant saponins in the leaf of S. officinalis were ursolic and oleanolic acids, the total content of which is 75.82 %. In the leaves of S.grandiflora, ursolic and euskapic acids were dominant, with a total content of 63.25 %.The total flavonoid content is higher in S. officinalis leaf and is 4.90 mg / g. The total content of hydroxycinnamic acids is highest in the leaf of S. grandiflora and is 4.49 mg / g, which is 221.18 % (2.21 times) higher than in the pharmacopeia plant S. officinalis (2.03 mg / g). The total highest content of caffeic acid derivatives prevails in the Salvia officinalis leaf (0.77 mg / g). The highest content of the sum of all detected phenolic compounds is specified for S. officinalis leaves and amounts to 6.93 mg / g.Conclusions. As a result of a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis, it was established that S. grandiflora is a promising species for introduction into medical and pharmaceutical practice, namely, as a source of phenolic compound