6 research outputs found


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    The Benefits of Nori Fruit Extract (Morinda Citrifolia Linn) in Increasing the Storage of Spermatozoa Y Bali Cows Resulting from Sexing at a Temperature of 5oC

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    The sperm sexing technique is the separation of spermatozoa carrying the X chromosome and the Y chromosome, which allows farmers to choose male births for beef cattle businesses. However, the process of centrifugation and sperm preservation can cause oxidative stress which gives rise to free radicals. Therefore, an antioxidant ingredient is needed in the diluent, including noni fruit extract which contains lots of flavonoids. This research aims to analyze the addition of noni fruit extract in a diluent to improve the quality of Y-chromosome spermatozoa at a storage temperature of 5oC. The results of the study showed the results of analysis of the progressive motility of Y-chromosome spermatozoa, showing a decrease in progressive motility, on D7 and D-9 there was a difference between control vs P1 and P2 (P<.05) respectively 37.63% vs 49.96% vs 48.56%, while on day 11 showed a very real difference between P0 vs P1 vs P2 respectively 24.32% vs 36.77% vs 40.87%. The addition of 3% and 5% noni extract can improve the quality of Y spermatozoa stored at 5oC. This is because noni extract contains amino acids, ascorbic acid, and flavonoids as antioxidants so that it is able to prevent free radicals, scavengers, and damage to the spermatozoa plasma membrane can be prevented. The addition of 5% noni fruit extract in the diluent to improve the quality of Y-chromosome spermatozoa at a storage temperature of 5oC for up to 11 days

    Effect of Insulin Transferin Selenium (ITS) on oocyte maturation in vitro in Indonesian goats

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    The aim of the present study was to evaluate the effects of Insulin-Transferin-Selenium (ITS) on maturation of oocytes in vitro in Indonesian Goats. Goat ovaries were collected at a local slaughterhouse and washed twice in sterilized NaCl solution. The follicular content including cumulus-oocyte complexes (COCs), were collected by aspirating the visible small antral follicles (around 2-6 mm diameter) with a 10 ml syringe equipped with an 8-gauge needle. The oocytes with more two layers were cultured for 28 hours at 38.5oC and 5%CO2. After maturation the cumulus cells were removed and the denuded oocytes were stained with 2% aceto-orcein to determine the nuclear oocyte status. The result showed that the rate of nuclear maturation in ITS-treated groups was significantly higher (P<0.05) than in the ITS-untreated group, were 38.14%a;  61,40%b and 71,55%b ,  respectively for medium without ITS,  5 ng/ml ITS and  10 ng/ml ITS.  This observation indicated that supplementation of ITS into culture medium was effectively enhance oocyte nuclear maturation in vitro. Keywords:  oocyte, nuclear maturation, Insulin Transferin Selenium, in vitro maturatio


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    The processing of liquid cement is likely to decrease sperm motility. It can be caused by the oxidative reaction to increase the amount of free radicals. Noni (Morinda citrifolia Linn) contains many antioxidants that can serve counteract free radicals. This study aims to determine the addition of extracts of noni (Morinda citrifolia Linn) in order to maintain the individual motility of spermatozoa in the semen liquid Bali cattle. This research was conducted at the Laboratory of Animal Reproduction Semen Processing Unit Faculty of Animal Husbandry Hasanuddin University Makassar. Extracts of noni fruit (Morinda citrifolia Linn) effect on sperm motility (P <0.05). Storage P0 at 3 h to day 2 showed higher motility. Concentration 0,02 gr/ml (P1) gives the best results in maintaining sperm motility on day 3rd to 5th. Extracts of noni fruit (Morinda citrifolia Linn) can retain sperm motility because many contain many antioxidants that serve to neutralize free radicals cause a decrease in sperm motility during equilibration at 5 0C

    Higher Education and Sustainability of Beef Cattle in the Village of South Sulawesi Indonesia

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    Bone Regency is an agricultural area that includes food crops, livestock, and fisheries. This area is a very large producer of livestock, so this study analyzed how the influence of tertiary institutions in their role in the form of community service in the form of assistance to groups of farmers in the area, especially in the village of Bune. In this study using the method used quantitatively to see the role of tertiary institutions, data were obtained through a questionnaire with a sample of 30 people and the statistical tools used were Path Analysis is to compare all the variables used so that there is a picture of the role of tertiary institutions in the development of farmers' businesses in the village of Bune. The results of the research show that with the assistance provided by tertiary institutions, it can improve the competence of cattle ranching, namely technically, entrepreneurial and managerial abilities, from these variables after being analyzed using statistics shows that technical competence, entrepreneurship, and managerial influence the increase in productivity, so that the cattle farming business managed by the community in Bune Village can be sustainable