17 research outputs found

    Frequ√™ncia de polimorfismos do gene TMEM18 numa popula√ß√£o de crian√ßas participantes de um estudo de coorte em Salvador ‚Äď BA

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    Introdu√ß√£o: O TMEM18 √© um gene localizado no cromossomo 2p25.3. A recente liga√ß√£o deste gene √† obesidade tem sido replicada em diversos estudos de associa√ß√£o gen√īmica para algumas variantes al√©licas. O presente artigo analisou as frequ√™ncias al√©licas e genot√≠picas de polimorfismos no gene TMEM18 numa amostra da popula√ß√£o de Salvador ‚Äď BA, e a diferen√ßa das MAFs encontradas em rela√ß√£o √†s principais popula√ß√Ķes que respondem pela composi√ß√£o √©tnica do Brasil ‚Äď amer√≠ndios, africanos e europeus. Metodologia: participaram deste estudo 1308 crian√ßas com idade entre 4 a 11 anos, integrantes de um estudo de coorte do projeto Social Changes, Asthmaand Allergy in LatinAmerica (SCAALA). Para a genotipagem dos indiv√≠duos foi utilizado o painel comercial Illumina Human Omni 2, com aproximadamente 2.5 milh√Ķes de marcadores. Utilizamos os softwares PLINK (PURCELL et al., 2007)¬† e Haploview (BROAD Institute) para controle de qualidade dos SNPs e an√°lise de agrega√ß√£o de hapl√≥tipos (Linkage Desequilibrium - LD>4.2%), respectivamente. Resultados: As variantes do gene TMEM18 encontradas localizam-se na regi√£o 3¬īUTR (rs17729501, rs17042334, rs3187671) e intr√īnica (rs73153245, rs73153246, rs4241323, rs12990777, e rs2293084). Todos os polimorfismos est√£o em equil√≠brio de Hardy-Weinberg. As frequ√™ncias dos alelos menos frequentes (MAFs) observadas (9% e 9,3%) foram para os SNPs rs17729501 ers17042334, respectivamente. ¬†Os polimorfismos com as menores MAFs - s17729501 (9%) e rs17729501 (9,3%) - apresentaram, simultaneamente, as menores frequ√™ncias genot√≠picas para homozigose, 0,5% (C/C) e 0,8% (T/T), respectivamente. As maiores taxas de heterozigose observadas na popula√ß√£o foram de 44% (A/C - rs2293084) e 47,3% (G/A -rs12990777) e correspondem √†s maiores frequ√™ncias al√©licas (rs2293084 ‚Äď 34,9%; rs12990777 - 47,3%). Os SNPs rs3187671, rs73153245 e rs73153246 est√£o em desequil√≠brio de liga√ß√£o. Conclus√£o: As frequ√™ncias al√©licas obtidas para os SNPs, de uma forma geral, diferiram das frequ√™ncias registradas para popula√ß√Ķes de refer√™ncia. O presente trabalho contribuiu com informa√ß√Ķes sobre a ocorr√™ncia de polimorfismos no TMEM18 na popula√ß√£o brasileira, bem como com as frequ√™ncias al√©licas dos SNPs analisadas para os futuros estudos do tipo GWAS.

    Avaliação dos efeitos do extrato do cha verde (Camellia sinensis (L.) Kuntze) sobre a resposta imunohematopoetica de camundongos infectados com Listeria monocytogenes

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    Orientador: Mary Luci de Souza QueirozTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias MedicasResumo: Esse trabalho investiga o efeito protetor do tratamento com diferentes doses (50, 100 e 250 mg/Kg) do extrato padronizado de Ch√° Verde (GTE) em camundongos infectados com Listeria monocytogenes (LM). A efic√°cia terap√™utica foi avaliada para uma dose letal de LM por sete dias consecutivos com diferentes doses de GTE. A prote√ß√£o significativa do extrato foi verificada durante a fase aguda da infec√ß√£o e um mecanismo de a√ß√£o foi proposto a partir da an√°lise dos efeitos do GTE sobre o crescimento e a diferencia√ß√£o de progenitores hematopo√©ticos para granul√≥citos e macr√≥fagos (CFU-GM) da medula √≥ssea e do ba√ßo, sobre a atividade estimuladora de col√īnias (CSA) do soro, sobre a atividade l√≠tica de c√©lulas NK, sobre a prolifera√ß√£o de linf√≥citos e sobre a produ√ß√£o de citocinas de padr√£o Thl (IFN-y) / Th2 (IL-10) em animais normais e infectados com LM. Os resultados demonstraram que a resist√™ncia dos animais infectados com uma dose letal de LM tratados com GTE est√° relacionada com a modula√ß√£o da mielossupress√£o e diminui√ß√£o da hematopoese extramedular produzidas pela infec√ß√£o. Tamb√©m foi verificado um aumento da atividade l√≠tica de c√©lulas NK, da capacidade proliferativa de linf√≥citos e da polariza√ß√£o da resposta imunol√≥gica para Thl. O GTE n√£o apresentou toxicidade sobre o estroma medular e estimulou a gera√ß√£o de progenitores para granul√≥citos-macr√≥fagos em sistema de cultura l√≠quida de longa dura√ß√£o (LTBMC). O mecanismo de a√ß√£o da atividade imunomoduladora do GTE ainda n√£o est√° completamente definido. No entanto, os mecanismos de a√ß√£o apresentados neste trabalho s√£o de fundamental import√Ęncia para a atividade antibacteriana in vivo do GTEAbstract: In the present study we investigated the protective effects of the oral administration of Green tea (GTE) (50, 100 and 250mg/Kg) in mice infected with Listeria monocytogenes (LM). We demonstrated that GTE protects mice from a lethal dose of LM when administered prophylactically for seven consecutive days. To determine the mechanism of action of GTE, we examined the effect of GTE on the number of granulocyte and macrophage colony-forming units (CFU-GM) from bone marrow and spleen, on the colony-stimulating activity (CSA) of serum, on natural killer (NK) cell activity, on lymphocyte proliferation and on Thl/Th2-based cytokine production in normal and LM infected mice. Myelosuppression and extramedular hematopoiesis/splenomegaly were the major features of LM infection, in association with NK cell activation and alteration of lymphocyte proliferation. The resistance of mice infected with a lethal dose of LM but treated prophylactically with 100 and 250mg/Kg of GTE could be related to the modulation of myelosuppression and extramedular hematopoesis induced by the infection, and to an increase in NK cell activity, lymphocyte proliferation and polarization of the Thl-based immune response. The GTE extract was not toxic to either the stromal cell layer or to GM progenitors generated from long-term bone marrow culture (LTBMC). In this way, the mechanism of action through GTE exerts its pharmacological effect is not completely elucidated, although the parameters mentioned here are essential for its antibacterial effect in vivoDoutoradoDoutor em Farmacologi

    Teor de fenólicos totais e atividade antioxidante das sementes da Carpotroche brasiliensis (Raddi)

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    Introdu√ß√£o: A Carpotroche brasiliensis √© uma √°rvore nativa brasileira, utilizada na medicina popular. Objetivo: avaliar a qualidade nutricional, fatores antinutricionais, teor de compostos fen√≥licos e determinar atividade antioxidante das sementes da planta. Metodologia: As sementes foram divididas em amostra natural (CB) e liofilizada (CBL). A composi√ß√£o centesimal e teor de √°cido ox√°lico foram realizados em triplicata, segundo normas do Instituto Adolfo Lutz. O √°cido f√≠tico foi determinado por espectrofotometria, com reagente de Wade. Os fen√≥is foram determinados nos extratos aquosos e etan√≥lico por Folin-Dennis e expressos em √°cido g√°lico e t√Ęnico equivalentes.¬† A avalia√ß√£o antioxidante foi determinada pela capacidade em sequestrar radicais livres, utilizando o modelo in vitro do 2,2 difenil-1-picril hidrazil radical (DPPH) e expressa em IC50. An√°lise de regress√£o linear foi utilizada como avalia√ß√£o comparativa da a√ß√£o antioxidante e teor de fen√≥is. Resultados: As amostras apresentaram alto teor de lip√≠dios, 25g% para CB e 69g% para CBL. O teor de √°cido f√≠tico encontrado foi 1,43g% (dp=0,04) e de √°cido ox√°lico foi de 4,8mg% (dp=0,47) na CBL. O teor de fen√≥is variou de 367,5 a 598mg% em ambos os extratos. A IC50 nos extratos foi de 2298,0 √¨g/mL (dp=9,7) e 4150,7 √¨g/mL (dp=43,3) respectivamente para o extrato aquoso e etan√≥lico. O teor de fen√≥is foi diretamente proporcional ao porcentual da atividade antioxidante das amostras. Conclus√£o: As sementes apresentaram potencial para extra√ß√£o de √≥leo para fins farmac√™uticos ou nutricionais. Os valores de antinutrientes est√£o dentro da faixa dos teores encontrados em variados tipos de alimentos, habitualmente consumidos pela popula√ß√£o. ¬† Abstract Introduction: Carpotroche brasiliensis is a native Brazilian tree, used in folk medicine. Objective: To evaluate the nutritional quality, antinutritional factors, phenolic content and antioxidant activity from plant seeds. Methodology: The seeds were divided into natural (CB) and lyophilized (CBL) samples. The composition and content of oxalic acid were performed in triplicate, according to the Instituto Adolfo Lutz‚Äôs recommendations. Phytic acid was determined by spectrophotometry using Wade reagent. The phenols were determined in aqueous and ethanol extracts by Folin-Dennis and expressed as gallic acid and tannic acid equivalents. Evaluation was determined by the antioxidant capacity to scavenge free radicals using the in vitro model of 2,2 difenil-1-picril hidrazil radical (DPPH) and expressed as IC50. Linear regression analysis was used for a comparative evaluation of antioxidant activity and phenolic content. Results: The samples showed high content of fat, 25g%for CB and 69g% for CBL. The content of phytic acid was found to be 1.43 g% (dp=0.04) and oxalic acid was 4.8 mg% (dp=0.47) in the sample dried by lyophilization. The total phenolic content ranged from 367.5% to 598mg% in both extracts. The IC50 for antioxidant activity was 2298.0 mg/ mL (dp=9.7) and 4150.7 g / mL (dp=43.3), respectively, for the aqueous and ethanolic extracts. The phenol content was directly proportional to the percentage of antioxidant activity of the samples. Conclusion: Based on our results, Carpotroche brasiliensis‚Äôs seeds showed potential for oil extraction for pharmaceutical or nutritional uses. The values of antinutrients were equivalents to various types of foods usually consumed by the population

    Teor de fenólicos totais e atividade antioxidante das sementes da Carpotroche brasiliensis (Raddi)

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    Introdu√ß√£o: A Carpotroche brasiliensis √© uma √°rvore nativa brasileira, utilizada na medicina popular. Objetivo: avaliar a qualidade nutricional, fatores antinutricionais, teor de compostos fen√≥licos e determinar atividade antioxidante das sementes da planta. Metodologia: As sementes foram divididas em amostra natural (CB) e liofilizada (CBL). A composi√ß√£o centesimal e teor de √°cido ox√°lico foram realizados em triplicata, segundo normas do Instituto Adolfo Lutz. O √°cido f√≠tico foi determinado por espectrofotometria, com reagente de Wade. Os fen√≥is foram determinados nos extratos aquosos e etan√≥lico por Folin-Dennis e expressos em √°cido g√°lico e t√Ęnico equivalentes.¬† A avalia√ß√£o antioxidante foi determinada pela capacidade em sequestrar radicais livres, utilizando o modelo in vitro do 2,2 difenil-1-picril hidrazil radical (DPPH) e expressa em IC50. An√°lise de regress√£o linear foi utilizada como avalia√ß√£o comparativa da a√ß√£o antioxidante e teor de fen√≥is. Resultados: As amostras apresentaram alto teor de lip√≠dios, 25g% para CB e 69g% para CBL. O teor de √°cido f√≠tico encontrado foi 1,43g% (dp=0,04) e de √°cido ox√°lico foi de 4,8mg% (dp=0,47) na CBL. O teor de fen√≥is variou de 367,5 a 598mg% em ambos os extratos. A IC50 nos extratos foi de 2298,0 √¨g/mL (dp=9,7) e 4150,7 √¨g/mL (dp=43,3) respectivamente para o extrato aquoso e etan√≥lico. O teor de fen√≥is foi diretamente proporcional ao porcentual da atividade antioxidante das amostras. Conclus√£o: As sementes apresentaram potencial para extra√ß√£o de √≥leo para fins farmac√™uticos ou nutricionais. Os valores de antinutrientes est√£o dentro da faixa dos teores encontrados em variados tipos de alimentos, habitualmente consumidos pela popula√ß√£o. ¬† Abstract Introduction: Carpotroche brasiliensis is a native Brazilian tree, used in folk medicine. Objective: To evaluate the nutritional quality, antinutritional factors, phenolic content and antioxidant activity from plant seeds. Methodology: The seeds were divided into natural (CB) and lyophilized (CBL) samples. The composition and content of oxalic acid were performed in triplicate, according to the Instituto Adolfo Lutz‚Äôs recommendations. Phytic acid was determined by spectrophotometry using Wade reagent. The phenols were determined in aqueous and ethanol extracts by Folin-Dennis and expressed as gallic acid and tannic acid equivalents. Evaluation was determined by the antioxidant capacity to scavenge free radicals using the in vitro model of 2,2 difenil-1-picril hidrazil radical (DPPH) and expressed as IC50. Linear regression analysis was used for a comparative evaluation of antioxidant activity and phenolic content. Results: The samples showed high content of fat, 25g%for CB and 69g% for CBL. The content of phytic acid was found to be 1.43 g% (dp=0.04) and oxalic acid was 4.8 mg% (dp=0.47) in the sample dried by lyophilization. The total phenolic content ranged from 367.5% to 598mg% in both extracts. The IC50 for antioxidant activity was 2298.0 mg/ mL (dp=9.7) and 4150.7 g / mL (dp=43.3), respectively, for the aqueous and ethanolic extracts. The phenol content was directly proportional to the percentage of antioxidant activity of the samples. Conclusion: Based on our results, Carpotroche brasiliensis‚Äôs seeds showed potential for oil extraction for pharmaceutical or nutritional uses. The values of antinutrients were equivalents to various types of foods usually consumed by the population

    An improved method to obtain antigen-excreting Toxocara canis larvae.

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    Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-09-02T11:41:25Z No. of bitstreams: 1 Alcantara Neves NM An Improved method.......pdf: 349925 bytes, checksum: f6433ce20f8aafbdc11ef77037e107be (MD5)Made available in DSpace on 2014-09-02T11:41:25Z (GMT). No. of bitstreams: 1 Alcantara Neves NM An Improved method.......pdf: 349925 bytes, checksum: f6433ce20f8aafbdc11ef77037e107be (MD5) Previous issue date: 2008Universidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilFunda√ß√£o Oswaldo Cruz. Centro de Pesquisa Gon√ßalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Sa√ļde P√ļblica. Salvador, BA, BrasilToxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory‚Äďsecretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stools, the separation of eggs from dissected uteri, and the in vitro growing of egg-derived larvae, following the time-consuming and laborious protocol described by De Savigny [De Savigny, D.H., 1975. In vitro maintenance of T. canis larvae and a simple method for the production of Toxocara ES antigen for the uses in serodiagnostic tests for visceral larva migrans. Journal of Parasitology 61, 781‚Äď782]. In this work, an improved protocol for obtaining T. canis larvae is described. The modifications proposed improved the efficiency of the original De Savigny method in three ways: (i) increasing the parasite yield up to five fold, (ii) improving the larval purity, and (iii) markedly reducing the execution time of the protocol

    Adenylyl cyclase type 9 gene polymorphisms are associated with asthma and allergy in Brazilian children

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    Barreto, Maur√≠cio Lima. ‚ÄúDocumento produzido em parceria ou por autor vinculado √† Fiocruz, mas n√£o consta √† informa√ß√£o no documento‚ÄĚ.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-04-06T16:05:16Z No. of bitstreams: 1 Teixeira HM Adenylyl cyclase type 9 gene ....pdf: 1921826 bytes, checksum: a47e5fe042de9b5bc2df29ff069a8a29 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-04-06T16:11:36Z (GMT) No. of bitstreams: 1 Teixeira HM Adenylyl cyclase type 9 gene ....pdf: 1921826 bytes, checksum: a47e5fe042de9b5bc2df29ff069a8a29 (MD5)Made available in DSpace on 2018-04-06T16:11:36Z (GMT). No. of bitstreams: 1 Teixeira HM Adenylyl cyclase type 9 gene ....pdf: 1921826 bytes, checksum: a47e5fe042de9b5bc2df29ff069a8a29 (MD5) Previous issue date: 2017Universidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Sa√ļde Coletiva. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilAsthma is a chronic inflammatory disease of the respiratory tract. This heterogeneous disease is caused by the interaction of interindividual genetic variability and environmental factors. The gene adenylyl cyclase type 9 (ADCY9) encodes a protein called adenylyl cyclase (AC), responsible for producing the second messenger cyclic AMP (cAMP). cAMP is produced by T regulatory cells and is involved in the down-regulation of T effector cells. Failures in cAMP production may be related to an imbalance in the regulatory immune response, leading to immune-mediated diseases, such as allergic disorders. The aim of this study was to investigate how polymorphisms in the ADCY9 are associated with asthma and allergic markers. The study comprised 1309 subjects from the SCAALA (Social Changes Asthma and Allergy in Latin America) program. Genotyping was accomplished using the Illumina 2.5 Human Omni bead chip. Logistic regression was used to assess the association between allergy markers and ADCY9 variation in PLINK 1.07 software with adjustments for sex, age, helminth infection and ancestry markers. The ADCY9 candidate gene was associated with different phenotypes, such as asthma, specific IgE, skin prick test, and cytokine production. Among 133 markers analyzed, 29 SNPs where associated with asthma and allergic markers in silico analysis revealed the functional impact of the 6 SNPs on ADCY9 expression. It can be concluded that polymorphisms in the ADCY9 gene are significantly associated with asthma and/or allergy markers. We believe that such polymorphisms may lead to increased expression of adenylyl cyclase with a consequent increase in immunoregulatory activity. Therefore, these SNPs may offer an impact on the occurrence of these conditions in admixture population from countries such as Brazil

    Polymorphisms in DENND1B gene are associated with asthma and atopy phenotypes in Brazilian children

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    Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-02-28T19:15:53Z No. of bitstreams: 1 Fiuza BS Polymorphisms in DENND1B gene are associated....pdf: 785468 bytes, checksum: 752a3a4da4f9e5c39c215f56d208c14b (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-02-28T19:25:21Z (GMT) No. of bitstreams: 1 Fiuza BS Polymorphisms in DENND1B gene are associated....pdf: 785468 bytes, checksum: 752a3a4da4f9e5c39c215f56d208c14b (MD5)Made available in DSpace on 2018-02-28T19:25:21Z (GMT). No. of bitstreams: 1 Fiuza BS Polymorphisms in DENND1B gene are associated....pdf: 785468 bytes, checksum: 752a3a4da4f9e5c39c215f56d208c14b (MD5) Previous issue date: 2017CNPq ‚ąí National Research Council - Brazil and FAPESB ‚ąí State of Bahia, Brazil Research CouncilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Laborat√≥rio de Imunofarmacologia e Biologia Molecular. Departamento de Biorregula√ß√£o. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Laborat√≥rio de Imunofarmacologia e Biologia Molecular. Departamento de Biorregula√ß√£o. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Laborat√≥rio de Imunofarmacologia e Biologia Molecular. Departamento de Biorregula√ß√£o. Salvador, BA, BrasilFunda√ß√£o Oswaldo Cruz. Instituto Gon√ßalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Laborat√≥rio de Imunofarmacologia e Biologia Molecular. Departamento de Biorregula√ß√£o. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Laborat√≥rio de Imunofarmacologia e Biologia Molecular. Departamento de Biorregula√ß√£o. Salvador, BA, BrasilAsthma is a heterogeneous disease associated with a complex basis involving environmental factors and individual variabilities. The DENN Domain Containing 1B (DENND1B) gene has an important role on T cell receptor (TCR) down-regulation on Th2 cells and studies have shown that mutations or loss of this factor can be associated with increased Th2 responses and asthma. The aim of this work is to evaluate the association of polymorphisms in the DENND1B with asthma and allergy markers phenotypes in Brazilian children. Genotyping was performed using a commercial panel from Illumina (2.5 Human Omni bead chip) in 1309 participants of SCAALA (Social Change, Asthma, Allergy in Latin American) program. Logistic regressions for asthma and atopy markers were performed using PLINK software 1.9. The analyzes were adjusted for sex, age, helminth infections and ancestry markers. The DENND1B gene was associated with different phenotypes such as severe asthma and atopic markers (specific IgE production, skin prick test and IL-13 production). Among the 166 SNPs analyzed, 72 were associated with asthma and/or allergy markers. In conclusion, polymorphisms in the DENND1B are significantly associated with development of asthma and atopy and these polymorphisms can influence DENND1B expression and consequently, asthma

    Seroprevalence and risk factors for Toxocara infection in children from an urban large setting in Northeast Brazil.

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    Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-08-25T17:41:27Z No. of bitstreams: 1 Mendon√ßa L Seroprevalence and risk....pdf: 933259 bytes, checksum: 24aad95e3018ba4633656e6c3883ea2b (MD5)Made available in DSpace on 2014-08-25T17:41:27Z (GMT). No. of bitstreams: 1 Mendon√ßa L Seroprevalence and risk....pdf: 933259 bytes, checksum: 24aad95e3018ba4633656e6c3883ea2b (MD5) Previous issue date: 2013Universidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilCentro Integrado de Manufatura e Tecnologia. Servi√ßo Nacional de Aprendizagem Industrial. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Matem√°tica. Salvador, BA, BrasilFunda√ß√£o Oswaldo Cruz. Centro de Pesquisas Gon√ßalo Moniz. Salvador, BA, BrasilUniversidad San Francisco de Quito. Colegio de Ciencias de la Salud. Quito, Ecuador / Liverpool School of Tropical Medicine. Molecular and Biochemical Parasitology. Liverpool, UK / Pontif√≠cia Universidad Cat√≥lica Del Ecuador. Escuela de Biologia. Quito, EcuadorPontif√≠cia Universidad Cat√≥lica Del Ecuador. Escuela de Biologia. Quito, Ecuador / Universidade Federal da Bahia. Instituto de Sa√ļde Coletiva. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilOBJECTIVES: This study aimed to standardize an "in house" immunoassay to detect anti-Toxocara IgG antibodies in human serum to estimate the seroprevalence of Toxocara infection, and to identify its potential risk factors in children living in poor areas of Salvador, a large northeastern Brazilian city. METHODS: Parents of 1309 children answered a questionnaire containing possible risk factor for acquisition of this infection. Blood was collected and the presence of anti-Toxocara IgG antibodies was detected by indirect ELISA using T. canis larval excretory-secretory antigens in sera previously absorbed with Ascaris lumbricoides antigens. RESULTS: Seroprevalence of Toxocara infection was 48.4%. Children's age, low maternal schooling, contact with dogs and cats, and household located in paved streets were shown to be risk factors for Toxocara infection. CONCLUSIONS: The seroprevalence of Toxocara infection is high among children living in a poor urban setting of Brazil. The association of low maternal education with higher Toxocara infection supports studies showing that low socioeconomic status is a risk factor for the acquisition of this infection as a reflection of hygiene habits of the family. And both infected-dogs and cats may be involved in this parasite transmission in this children population

    Antigen mimicry between infectious agents and self or environmental antigens may lead to long-term regulation of inflammation.

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    Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-08-25T18:46:51Z No. of bitstreams: 1 Pontes-de-Carvalho LC Antigen mimicry....pdf: 462804 bytes, checksum: 82ff9efbd4604f06bc2199bb1fd76da4 (MD5)Made available in DSpace on 2014-08-25T18:46:51Z (GMT). No. of bitstreams: 1 Pontes-de-Carvalho LC Antigen mimicry....pdf: 462804 bytes, checksum: 82ff9efbd4604f06bc2199bb1fd76da4 (MD5) Previous issue date: 2013Funda√ß√£o Oswaldo Cruz. Centro de Pesquisa Gon√ßalo Moniz. Salvador, BA, BrasilFunda√ß√£o Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, BrasilFunda√ß√£o Oswaldo Cruz. Centro de Pesquisa Gon√ßalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, Brasil /Universidade Federal da Bahia. Instituto de Sa√ļde Coletiva. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Colegio de Ciencias de La Salud, Universidad San Francisco de Quito. Quito, Ecuador / Molecular and Biochemical Parasitology, Liverpool School of Tropical Medicine, Liverpool, UK / London School of Hygiene and Tropical Medicine. London, UKUniversidade Federal da Bahia. Instituto de Ci√™ncias da Sa√ļde. Salvador, BA, Brasi
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