Uncovering ceRNA integrated networks that associate with fertility in a photoperiod and temperature sensitive male sterile wheat line

The pollen fertility of photoperiod/temperature sensitive genic male sterile (P/TGMS) wheat is controlled by light and/or temperature. Circular RNA (circRNA) and long non-coding RNA (lncRNA) are known to participate in the development of anthers in plants, but their impact on male sterility in the P/TGMS line is not well understood. In this study, we carried out high-throughput sequencing to investigate the differential expression of lncRNAs and circRNAs and their biological functions in anthers of photo-thermosensitive genic male sterile (PTGMS) wheat line BS366-42L during the transition phase of male fertility under four different photoperiod and temperature treatments. Eight lncRNAs, 40 mRNAs and three circRNAs were screened out and thought as essential candidates that closely related to male sterility. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to predict the potential functions of differentially expressed RNAs. The results indicated that carbohydrate-related metabolism was important for male sterility in the wheat PTGMS line BS366-42L. lncRNA/circRNA-mRNA-miRNA (ceRNA) integrate networks were constructed to reflect their complex inner association with male sterility. Our study provides a systematic perspective on the potential function of RNAs in male fertility in PTGMS lines of wheat.</p

MOESM7 of Genomic identification and characterization of MYC family genes in wheat (Triticum aestivum L.)

Additional file 7: Table S4. Segmental duplication of TaMYC gene

MOESM5 of Genomic identification and characterization of MYC family genes in wheat (Triticum aestivum L.)

Additional file 5: Table S2. Numbers of cis-regulatory elements in the upstream promoter regions of TaMYC gene

Additional file 2: of Genome-wide identification and analysis of the COI gene family in wheat (Triticum aestivum L.)

Table S1. Summary of COI or COI-like genes in different plants. Table S2. Specific primers for TaCOI genes for qRT-PCR. Table S3. Promoter sequences of TaCOI genes. Table S4. Protein sequences, CDS sequences, and genomic sequences of TaCOI and COI or 12 COI-like loci in various plants. (DOCX 241 kb

MOESM1 of Genomic identification and characterization of MYC family genes in wheat (Triticum aestivum L.)

Additional file 1: Figure S1. Chromosomal localizations and syntenic relationships among TaMYC genes in Triticum aestivum, T. urartu and Ae. Tauschii. Lines in grey indicate tandem duplication. Lines in blue, green and orange indicate segmental duplicatio

MOESM4 of Genomic identification and characterization of MYC family genes in wheat (Triticum aestivum L.)

Additional file 4: Table S1. The primers used in this stud

MOESM2 of Genomic identification and characterization of MYC family genes in wheat (Triticum aestivum L.)

Additional file 2: Figure. S2. Consensus sequence and logos of motifs from wheat MYC protein

Additional file 1: of Genome-wide identification and analysis of the COI gene family in wheat (Triticum aestivum L.)

Figure S1. Consensus sequence and logos of motifs from wheat COI proteins. (TIF 2339 kb

MOESM3 of Genomic identification and characterization of MYC family genes in wheat (Triticum aestivum L.)

Additional file 3: File S1. Promoter sequences of MYC gene copie

MOESM6 of Genomic identification and characterization of MYC family genes in wheat (Triticum aestivum L.)

Additional file 6: Table S3. miRNA targeting prediction of TaMYC gene famil