Bioreactor operation shows haloplasticity of a synthetic nitrifying community, enabling urine treatment in space

Additional file 4: Figure S2. Detection of aabgl1 copy numbers in the transformants by qPCR analysis. Tef1Îą gene was used as a single copy control

Additional file 1 of Potential of Zymomonas mobilis as an electricity producer in ethanol production

Additional file 1. Additional figures and tables

MOESM1 of Condition-specific promoter activities in Saccharomyces cerevisiae

Additional file 1: Table S1. Primers used in this study. Table S2. Ranking of promoter strengths at log phase under various conditions. Table S3. Sequence analysis of the promoters. Table S4. Predicted transcription factor (TF) binding sites involved in the promoter sequences. Figure S1. The GFP fluorescence of yeast cells under the control of various promoters and in the presence or absence of hygromycin B. Figure S2. Correlation of yEGFP fluorescence and mRNA levels. Figure S3. Cell growth of S. cerevisiae BY4741 under various conditions. Figure S4. The promoter strengths in log-phase cells under different conditions

Electrochemical Control of Cell Metabolism Improves Ethanol Production of Zymomonas mobilis in an Electro-Fermentation System

This study investigated ethanol fermentation by Zymomonas mobilis in an electro-fermentation system (EFS) for efficient alcohol production, which increased the ethanol titer by 12.8% compared to fermentation without electricity input. The underlying mechanism was revealed by exploring the correlation among intracellular redox parameters such as NAD­(P)­H/NAD­(P)+ ratio, total antioxidant capacity, and cell membrane permeability. Transcriptome analysis further investigated that genes related to glucose uptake, ethanol, and succinic acid synthesis were upregulated in the cathode chamber, which promoted ethanol production. Two strategies to enhance EFS were proposed. First, the supplementation of electron shuttles (methylene blue, HNQ) can facilitate electron transport between electrodes and cells. Second, the manipulation of essential electricity-sensing genes (ZMO1211, ZMO1753) amplified the metabolic change. This study showed the possible use of electro-regulation for the efficient production of alcohol in Z. mobilis, which also provided insights into other microbial electrochemical processes in the future

Electrochemical Control of Cell Metabolism Improves Ethanol Production of Zymomonas mobilis in an Electro-Fermentation System

This study investigated ethanol fermentation by Zymomonas mobilis in an electro-fermentation system (EFS) for efficient alcohol production, which increased the ethanol titer by 12.8% compared to fermentation without electricity input. The underlying mechanism was revealed by exploring the correlation among intracellular redox parameters such as NAD­(P)­H/NAD­(P)+ ratio, total antioxidant capacity, and cell membrane permeability. Transcriptome analysis further investigated that genes related to glucose uptake, ethanol, and succinic acid synthesis were upregulated in the cathode chamber, which promoted ethanol production. Two strategies to enhance EFS were proposed. First, the supplementation of electron shuttles (methylene blue, HNQ) can facilitate electron transport between electrodes and cells. Second, the manipulation of essential electricity-sensing genes (ZMO1211, ZMO1753) amplified the metabolic change. This study showed the possible use of electro-regulation for the efficient production of alcohol in Z. mobilis, which also provided insights into other microbial electrochemical processes in the future

MOESM1 of Production of l-alanyl-l-glutamine by immobilized Pichia pastoris GS115 expressing Îą-amino acid ester acyltransferase

Additional file 1. Additional figures

Psychologic evaluation of patients undergoing dorsal sympathicotomy for the treatment of bilateral palmar and axillary hyperhidrosis [Valutazione psicologica in pazienti sottoposti a simpaticotomia selettiva per iperidrosi palmare ed ascellare bilaterale]

Additional file 1: Table S1. Yeast strains used in this study. Table S2. List of primers used for RT-qPCR analysis in this work. Table S3. List of primers used for plasmids and strains construction in this work. Fig. S1. Influence of acetic acid stress on transcription of ADE17 in different S. cerevisiae BY4741 strains. Fig. S2. Comparison of stress tolerance of the engineered yeast strains with that of the control strain BHO under various stressful conditions. Fig. S3. Potential transcription factors regulating the ADE genes. Fig. S4. Growth curve of the recombinant strains under various conditions. Fig. S5. Detoxification of furfural and 5-HMF by the recombinant yeast strains. Fig. S6. Effect of ADE genes overexpression on intracellular energy level at stationary phase. Fig. S7. Effect of amino acids addition on yeast growth under acetic acid stress condition. Fig. S8. Comparison of acetic acid tolerance of the mutant strain ADE17_mZRE and the control strain. Fig. S9. Impact of the ADE genes overexpression on succinic acid production

Data_Sheet_1_Genome Mining of the Marine Actinomycete Streptomyces sp. DUT11 and Discovery of Tunicamycins as Anti-complement Agents.docx

<p>Marine actinobacteria are potential producers of various secondary metabolites with diverse bioactivities. Among various bioactive compounds, anti-complement agents have received great interest for drug discovery to treat numerous diseases caused by inappropriate activation of the human complement system. However, marine streptomycetes producing anti-complement agents are still poorly explored. In this study, a marine-derived strain Streptomyces sp. DUT11 showing superior anti-complement activity was focused, and its genome sequence was analyzed. Gene clusters showing high similarities to that of tunicamycin and nonactin were identified, and their corresponding metabolites were also detected. Subsequently, tunicamycin I, V, and VII were isolated from Streptomyces sp. DUT11. Anti-complement assay showed that tunicamycin I, V, VII inhibited complement activation through the classic pathway, whereas no anti-complement activity of nonactin was detected. This is the first time that tunicamycins are reported to have such activity. In addition, genome analysis indicates that Streptomyces sp. DUT11 has the potential to produce novel lassopeptides and lantibiotics. These results suggest that marine Streptomyces are rich sources of anti-complement agents for drug discovery.</p

MOESM2 of Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic ethanol production from corn stover

Additional file 2: Table S2. Identified secreted proteins induced by MGD and CS

MOESM4 of Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic ethanol production from corn stover

Additional file 4: Figure S2. Detection of aabgl1 copy numbers in the transformants by qPCR analysis. Tef1Îą gene was used as a single copy control