DOSY NMR: A Versatile Analytical Chromatographic Tool for Lignocellulosic Biomass Conversion

The diffusion ordered NMR spectroscopy (DOSY) protocol for the analysis of reaction mixture of lignocellulosic biomass conversion has been developed and investigated systematically. Model reaction mixtures from cellulose, hemicellulose and lignin conversion, real reaction mixtures of sucrose and glucose dehydration, were facilely separated and assigned in the diffusion dimension without any prior separation or isolation. The shift reagent, EuFOD, was successfully utilized to increase the difference in diffusion and thereby resolution in lignin degradation model. DOSY NMR offers an easy and robust method for the structure identification and reaction mixture separation in biomass conversion