7 research outputs found

    Negative correlation between expressions of an up-regulated set of miRNAs and their targeted chemokine transcripts.

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    <p>Expression means of let-7a, miR-25, miR-26a, miR-140, miR-146a and miR-155 at 3 h and miR-23b and miR-132 at 6 h post-infection of three healthy donors (D1, D2 and D3; panel A) is negatively correlated with CCL2, CCL5, CXCL10, CXCL11 and CXCL12 mRNA mean levels at 12 and 24 h post-infection (panel B) in <i>L. major</i>-infected human macrophages. Results were expressed using the 2<sup>−ΔΔCt</sup> method.</p

    Time course of miRNA-210 and procaspase-3 expression levels in <i>L. major</i> infected human primary macrophages.

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    <p>MiR-210 expression at 3, 6, 12, and 24 h in infected cells relatively to non-infected cells (panel A), after siRNA-control or HIF-1α-silencing transfections in non-infected and infected cells (panel B). Results were expressed using the 2<sup>−ΔΔCt</sup> method. Stars (*) are indicated when results are statistically significant from control. One star indicates a <i>p</i> value <0.05; two stars indicate a <i>p</i> value <0.01 and three stars indicate a <i>p</i> value <0.001. Panel C represents abundance of pro-caspase-3 protein levels in time course parasite-infected macrophages of healthy donors revealed by western blot analysis. HSP27 was used as loading control. Ten µg of <i>L. major</i> lysate (latest lane) was used a negative control to ensure that anti-procaspase-3 antibody does not cross-react with parasite proteins. Data are representative of three independent experiments conducted on MDM derived from two to three different healthy donors. NI indicates non-infected cells and IF indicates infected cells.</p

    Biological processes deduced from analysis of deregulated miRNA-targets in <i>L. major</i>-infected human macrophages at 3 h post-infection.

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    <p>Yellow color gradient intensity correlates with up- or down-regulation levels. White nodes are not significantly overrepresented. The area of each node is proportional to the number of genes in the set annotated to the corresponding GO category. Interactions were visualized as a network using Cytoscape and BINGO plugin.</p

    Virtual classroom using the Mconf web conferencing platform.

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    <p>Classrooms connected to the trainer and to other classrooms via the Mconf open-source web conferencing platform (<a href="http://mconf.org/" target="_blank">http://mconf.org</a>; in use here is the South African instance of Mconf <a href="https://mconf.sanren.ac.za/" target="_blank">https://mconf.sanren.ac.za/</a>, hosted by SANREN <a href="http://www.sanren.ac.za/south-african-nren/" target="_blank">http://www.sanren.ac.za/south-african-nren/</a>). Classrooms either activated their microphones or entered text into a chat box to ask questions to the trainer. Trainers activated their webcams while answering questions. Trainers were able to upload their session resources in the central panel of the Mconf interface should they have wanted to explain a concept on a particular lecture slide, for example. Note: Consent to publish this image was obtained from the trainer shown in the figure. Abbreviation: SANREN, South African National Research Network.</p

    Screenshots of Vula site for IBT_2016 course.

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    <p>(A) Landing page of course site showing all tools. (B) Forums tool specifically showing the number of people that read the response to a particular question. In this example, at the time that the screenshot was obtained, 14 people had read the question, and 12 people had read the answer. Please note: Consent to publish this screenshot was obtained from participants of the relevant discussion. Abbreviation: IBT, Introduction to Bioinformatics.</p