Research on the Integrated Training Mode of Higher Art Education for the Deaf

Abstract: The development of higher education for the disabled is one of the most important indicators of the development of education for the disabled in a country, whether the support policies are perfect, and whether the social civilization is advanced. The results showed that deaf students with general school experience had better adaptive ability The integration model was more conducive to improve deaf students’ school adaptive ability and social interaction awareness. The deaf students had higher demand for professional guidance and there is room for further improvement of the integration-training model. Based on the above research results and the problems in practice, this paper gives the corresponding countermeasure suggestions. The paper suggests that we should build a “trinity” integration education model oriented to socially integrated talents in art applications and improve the management system of integration education We should also improve the deaf college students’ learning support system under the leadership of the school, modify and reconstruct the integration curriculum system of arts and crafts majors, and strengthen the support and guidance of the society for the construction of integration environment.</div

Additional file 2 of Circ_0072083 interference enhances growth-inhibiting effects of cisplatin in non-small-cell lung cancer cells via miR-545-3p/CBLL1 axis

Additional file 2: Figure S2. Circ_0072083 intervention has no significant effects on the colony formation and apoptosis of NSCLC cells. a The knockdown efficiency of si-circ_0072083 in BEAS-2B cells was examined by qRT-PCR. b Colony formation assay was employed to assess the colony formation ability of NSCLC cells transfected with si-NC or si-circ_0072083. c The apoptosis rate of NSCLC cells transfected with si-NC or si-circ_0072083 was evaluated by flow cytometry. *P < 0.05

Additional file 3 of Circ_0072083 interference enhances growth-inhibiting effects of cisplatin in non-small-cell lung cancer cells via miR-545-3p/CBLL1 axis

Additional file 3: Figure S3. Circ_0072083 interference enhances the inhibitory effect of cisplatin on the malignance of NSCLC cells via miR-545-3p/CBLL1 axis

Additional file 1 of Circ_0072083 interference enhances growth-inhibiting effects of cisplatin in non-small-cell lung cancer cells via miR-545-3p/CBLL1 axis

Additional file 1: Figure S1. The influence of DDP, circ_0072083, miR-545-3p and CBLL1 on the necrosis of NSCLC cells. a The viability of NSCLC cells treated with si-NC, si-circ_0072083, DDP + si-NC or DDP + si-circ_0072083 was detected by LDH cytotoxicity assay kit. b LDH cytotoxicity assay kit was used to measure the necrosis of DDP-induced NSCLC cells transfected with si-NC, si-circ_0072083, si-circ_0072083 + anti-miR-NC or si-circ_0072083 + anti-miR-545-3p. c The viability of DDP-treated NSCLC cells transfected with miR-NC, miR-545-3p, miR-545-3p + vector or miR-545-3p + CBLL1 was determined through using LDH cytotoxicity assay kit. *P < 0.05

Leader- and Terminal Residue Requirements for Circularin A Biosynthesis Probed by Systematic Mutational Analyses

Circularin A is a circular bacteriocin belonging to a subgroup of the ribosomally synthesized and post-translationally modified peptide (RiPP) superfamily. The post-translational biosynthesis of circular bacteriocins primarily consists of leader cleavage, core peptide circularization, and bacteriocin secretion. However, none of these processes have been fully elucidated due to the complex biosynthesis of such bacteriocins and the lack of homology to the functions of other known biosynthetic enzymes. In this study, we investigated the leader- and terminal residue requirements for the biosynthesis of circularin A by systematic mutational analyses, including the mutational effects of variable leader lengths, as well as site-directed substitutions of residues at positions near the leader cleavage site and the circularization site. Results show that the leader with only one Met residue, the shortest leader possible, is sufficient to produce mature circularin A; helix-forming short-sidechain hydrophobic residues are required at positions Val1 and Ala2 of the N-terminus to form active peptide derivatives, indicating the possible steric hindrance effect at these two positions; and an aromatic residue is required at the C-terminal Tyr69 position to produce a mature circular derivative. However, the requirements for residues at position Ala68 are much more relaxed relative to the positions of Val1 and Ala2, since even substitution with the largest possible residue, i.e., tryptophan, still allows the generation of an active Ala68Trp derivative. Our findings provide new perspectives for the biosynthesis of this short-leader circular bacteriocin, which enables the application of circular bacteriocin biosynthesis in rational modified peptide engineering

Data_Sheet_1_Engineering circular bacteriocins: structural and functional effects of α-helix exchanges and disulfide introductions in circularin A.PDF

Circular bacteriocins form a distinct group of antimicrobial peptides (AMPs) characterized by their unique head-to-tail ligated circular structure and functional properties. They belong to the ribosomally synthesized and post-translationally modified peptide (RiPP) family. The ribosomal origin of these peptides facilitates rapid diversification through mutations in the precursor genes combined with specific modification enzymes. In this study, we primarily explored the bacteriocin engineering potential of circularin A, a circular bacteriocin produced by Clostridium beijerinckii ATCC 25752. Specifically, we employed strategies involving α-helix replacements and disulfide bond introductions to investigate their effects on both biosynthesis and bioactivity of the bacteriocin. The results show the feasibility of peptide engineering to introduce certain structural properties into circularin A through carefully designed approaches. The introduction of cysteines for potential disulfide bonds resulted in a substantial reduction in bacteriocin biosynthesis and/or bioactivity, indicating the importance of maintaining dynamic flexibility of α-helices in circularin A, while reduction of the potential disulfide in one case increased the activity. The 5 α-helices of circularin A were respectively replaced by corresponding helices from another circular peptide, enterocin AS-48, and modestly active peptides were obtained in a few cases. Overall, this study provides valuable insights into the engineering potential of circular bacteriocins as antimicrobial agents, including their structural and functional restrictions and their suitability as peptide engineering scaffolds. This helps to pave the way for the development of novel antimicrobial peptides with tailored properties based on circular bacteriocins.</p

Physicochemical Characterization of MF_<i>m</i>^–‑Based Ammonium Ionic Liquids

A series of ammonium-based ionic liquids (ILs), which share a homologous series of cations (CH₃CH₂)₃N⁺(C_<i>n</i>H_2<i>n</i>+1) with <i>n</i> = 2, 4, 6, 8 and the anions with either BF₄^–, PF₆^–, or SbF₆^–, was synthesized. Their structures were confirmed by ¹H and ¹³C NMR, ESI-MS, and elemental analysis. Meanwhile, the content of impurity (e.g., water and bromide ions) was also determined using Karl Fischer titrator and ion chromatography. The thermal properties of the ILs were determined by TGA and DSC. Five of the investigated ILs have been shown to have a low melting point (< 100 °C): <i>N</i>,<i>N</i>,<i>N</i>,<i>N</i>-tetraethylammonium tetrafluoroborate, [N₂₂₂₂]­BF₄, <i>N</i>,<i>N</i>,<i>N</i>,<i>N</i>-tetraethylammonium hexafluorophosphate, [N₂₂₂₂]­PF₆, <i>N</i>,<i>N</i>,<i>N</i>-triethylhexylammonium tetrafluoroborate, [N₂₂₂₆]­BF₄, <i>N</i>,<i>N</i>,<i>N</i>-triethyloctylammonium hexafluorophosphate, [N₂₂₂₈]­PF₆ and <i>N</i>,<i>N</i>,<i>N</i>-triethyloctylammonium hexafluoroantimonate, [N₂₂₂₈]­SbF₆. Densities, refractive indices, and miscibility of these 12 ILs were well studied systematically. Moreover, from the analysis of the structure–property relationship, the role of the alkyl chain length of the cation on these physical properties of the ILs has been assessed, and the influence of the nature of the anions on these experimental data of the ILs has been discussed. The studies may provide valuable contributions for the design and study of ILs

Table_1_Inductive Reasoning Differs Between Taxonomic and Thematic Contexts: Electrophysiological Evidence.docx

Inductive reasoning can be performed in different contexts, but it is unclear whether the neural mechanism of reasoning performed in a thematic context (e.g., bee has x, so honey has x) is the same as that performed in a taxonomic context (e.g., bee has x, so butterfly has x). In the present study, participants were required to judge whether a conclusion was acceptable or not based on its premise, for which the taxonomic or thematic distances between premise and conclusion objects were either far or near. The Event related potential (ERP) results indicated that the effect of context (taxonomic vs. thematic) was initially observed in the P2 component; while the distance effect (far vs. near) was observed in N400 and late components. Moreover, the distance effect on thematic-based inductive reasoning was found in the anterior regions, while the distance effect on taxonomic-based inductive reasoning conditions was found in the posterior regions. These results support the view that inductive reasoning is performed differently under different semantic contexts.</p

Thermal-Responsive Photonic Crystal with Function of Color Switch Based on Thermochromic System

Responsive photonic crystals have attracted considerable attention. The responsiveness is usually achieved through the variation of reflection wavelengths based on Bragg diffraction. However, distinguishing external stimuli from intrinsic angle dependence is a challenge. Herein, a novel thermal-responsive photonic crystal was constructed based on the synergistic effect of the low-angle dependence of SnO2 inverse opals and a thermochromic phase change system. The organic thermochromic phase change system was obtained by mixing the fluoran dye (heat-sensitive red TF-R2), bisphenol A, and aliphatic alcohols in a certain proportion. By filling the thermochromic phase change system into SnO2 inverse opals, the thermal-responsive photonic crystal was fabricated. Through simple external thermal stimulation, the mutual transformation of low-angle-dependent structural color and pigmentary color is realized and inverse opal patterns can be displayed and hidden. The proposed system, while preventing the interference of the observation angle to the thermal stimulation, shows potential application prospect in the fields of anti-counterfeiting and information encryption fields

Elucidating the active ingredients and potential anti-inflammatory mechanism of Qingwei Huanglian Wan

Qingwei Huanglian Wan (QHW) is a traditional Chinese medicine used for treating inflammation. As the compositions of QHW preparations are complex and varied, it has proven difficult to identify the active ingredients in QHW and control the quality. In this study, high-performance liquid chromatography (HPLC) fingerprints, network pharmacology, molecular docking, and the Griess method were used to screen for and verify the potential active ingredients of QHW. First, sixteen batches of QHW were studied as two groups using multivariate statistical analysis. Thirteen peaks were detected in the HPLC results to establish a fingerprint similarity model, and six chemical constituents (phellodendrine hydrochloride, geniposide, berberine hydrochloride, baicalin, wogonoside, and glycyrrhizic acid) were identified. Among these six constituents, four components (glycyrrhizic acid, berberine hydrochloride, baicalin, and wogonoside) were considered as potential active ingredients. Second, network pharmacology, molecular docking, and the Griess method were used to further elucidate the potential active ingredients. There was no significant difference in the NO content of the active ingredients administration group (dose converted into the medium-dose group) and medium-dose QHW administration group. Then, the contents of berberine hydrochloride, baicalin, wogonoside, and glycyrrhizic acid were determined using the HPLC method. This study provides a comprehensive and reliable strategy for the quality control of QHW preparations and identifies potential active ingredients in QHW. Our methodology may also be useful for studying other traditional Chinese medicines. Elucidating the active ingredients and potential anti-inflammatory mechanism of Qingwei Huanglian Wan</p