Analysis of ergonomics problems contact-centers

The analysis of the ergonomic problems of the contact center. It allocates main factors of influence on man- operator

The STIM1 Phosphorylation Saga

Store-operated Ca2+ entry (SOCE) is a Ca2+ influx pathway present in practically every cell type in metazoans and mediates a variety of physiological functions. Defects in SOCE are associated with immunodeficiencies and defects in skeletal muscle development and function. The molecular machinery underpinning SOCE can be complex and cell type specific, however the minimal functional SOCE unit consists of the endoplasmic reticulum (ER) Ca2+ sensor STIM1 and the plasma membrane (PM) Ca2+-selective channel Orai1. STIM1 localizes to ER-PM contact sites (CS) following store depletion, where it recruits and gates Orai1. STIM1 is a phosphoprotein that is hyper-phosphorylated during cell division. STIM1 phosphorylation has been implicated in several functions, including modulation of cellular metabolism, SOCE inactivation during M-phase, ER segregation during mitosis, modulation of SOCE levels, and cell migration. However, the role of STIM1 phosphorylation in the majority of these processes is controversial bringing into question the physiological function of STIM1 phosphorylation, if any. Here we review the role and modulation of STIM1 phosphorylation under various conditions and argue that except for the modulation of energy metabolism, the physiological function of STIM1 phosphorylation remains unclear.Other InformationPublished in: Cell CalciumLicense: http://creativecommons.org/licenses/by/4.0/See article on publisher's website: https://dx.doi.org/10.1016/j.ceca.2022.102551</p

Immunosensor with Self-Referencing Based on Surface Plasmon Diffraction

We propose a novel type of immunosensor based on the diffraction of surface plasmon, which allows for in situ, real-time, and label-free observation of interfacial binding events. The inherent self-referencing mechanism of surface diffraction was found to be very effective for compensating fluctuations of the bulk, demonstrated by a temperature variation experiment. Possessing a stable baseline signal, the diffraction sensor offered picomolar sensitivity in directly detecting the binding of human chorionic gonadotropin (hCG) hormone

Calculation of out-of-pocket cap based on five-level family income.

Calculation of out-of-pocket cap based on five-level family income.</p

Kstar, an instance (similarity) based classifier for regression analysis had the greatest prediction power for ADOS module score for ASD children among all the regressors combining 4 selected imaging features.

<p>A1–A4: standardized histogram distribution showing x-axis as the z-scores of each feature and y-axis being the frequency. A1-right caudate volume, A2-average Z of caudate fcMRI, A3-average Z of IFG opercularis fcMRI, A4-average Z of IFG triangularis fcMRI. A5: phenotypic ADOS module score histogram distribution with x-axis as the original score and y-axis being the frequency. B: The correlation coefficient between integrated imaging features and ADOS module score improved largely from −0.64 based on single best imaging feature to 0.95 combining 4 imaging features. The fitted score using Kstar classifier with 4 selected imaging features showed a tight coupling (linear slope = 1; r = 0.95, P<0.0001) with the original ADOS module score with only a few outliers.</p

Improvement of the reduction in catastrophic health expenditure in China’s public health insurance

<div><p>This study aimed to locate the contributing factors of Catastrophic Health Expenditure (CHE), evaluate their impacts, and try to propose strategies for reducing the possibilities of CHE in the context of China’s current public health insurance system. The financial data of all hospitalization cases from a sample hospital in 2013 were gathered and used to determine the pattern of household medical costs. A simulation model was constructed based on China’s current public health insurance system to evaluate the financial burden for medical service on Chinese patients, as well as to calculate the possibilities of CHE. Then, by adjusting several parameters, suggestions were made for China’s health insurance system in order to reduce CHE. It’s found with China’s current public health insurance system, the financial aid that a patient may receive depends on whether he is from an urban or rural area and whether he is employed. Due to the different insurance policies and the wide income gap between urban and rural areas, rural residents are much more financially vulnerable during health crisis. The possibility of CHE can be more than 50% for low-income rural families. The CHE ratio can be dramatically lowered by applying different policies for different household income groups. It’s concluded the financial burden for medical services of Chinese patients is quite large currently, especially for those from rural areas. By referencing different healthcare policies in the world, applying different health insurance policies for different income groups can dramatically reduce the possibility of CHE in China.</p></div

Displacement of Molecules near a Metal Surface as Seen by an SPR−SPFS Biosensor

Movement of a fluorophore-labeled antibody on the surface of a self-assembled monolayer (SAM) was observed by surface plasmon resonance and surface-plasmon field-enhanced fluorescence spectroscopy (SPFS). At an extremely low surface coverage, the antibody lies close to the biotin-functionalized SAM surface. As additional nonlabeled antibodies were bound, the fluorophore-labeled antibody was displaced away from the SAM surface (and thus the gold surface) due to the constraint imposed by the neighboring nonlabeled antibody. A greater SPFS fluorescence signal was observed due to the weaker fluorescence quenching at large distances from the gold surface. The magnitude of the displacement is proportional to the available biotin binding sites on the sensor surface. The displacement is theoretically explained on the basis of the relationship between the fluorescence intensity and the evanescent field amplitude within the dielectric medium

CHE cases in different reimbursement policies.

CHE cases in different reimbursement policies.</p

Regions with significant volume percentage increment (A, red color) in gray matter, and volume decrement in gray matter (A, blue color) as well as white matter volume reduction (B, blue color) in autism compared to controls (P<0.01).

<p>Significant cortical thickness increment in percentage change in autism compared to controls is shown in C with red color (P<0.01). Both medial and lateral views on sagittal surface in the left and right hemispheres of an individual control subject are shown for gray matter volume (A), white matter volume (B) and cortical thickness (C) comparison between autistic children and controls. The percentage change of volume in autism was calculated as the mean difference between volumes of two groups normalized with individual supratentorial volume and scaled by the mean normalized volume of control group.</p

Differences between dilution and microscale culture metabolomic profiles.

(A) Heat-maps showing unsupervised hierarchical clustering of diluted (left panel) and microscale culture (right panel) metabolite peak intensities. Each column in heat maps indicates a technical or biological replicate. Blue, red and green colored bar on the top of heat-maps indicate different groups used for analysis. (B) PLS-DA plot showing differential clustering of dilution (105 and 104 cells)and microscale-cultured samples (24 well and 96 well) based on total metabolite peak intensities. Individual clusters contain three technical or biological replicates as indicated by different color signs.</p