The genetic landscape of the Iberian red deer (Cervus elaphus hispanicus) after 30 years of big-game hunting in southern Spain

© 2015 The Wildlife Society. The Iberian red deer (Cervus elaphus hispanicus) suffered a striking collapse of its populations during the first half of the 20th century due to excessive hunting. In Andalusia, southern Spain, re-colonization took place from a few relict populations through natural dispersal, and through artificial reintroductions for big-game hunting. How the population decline influenced genetic diversity, and its current distribution after the re-colonization and intensive hunting practices are unclear. We addressed these questions by analyzing nuclear microsatellite variability from 58 red deer populations distributed throughout Andalusia. Our results showed a relatively high genetic variability spatially structured into 5 clusters, corresponding to the locations of relict populations. This indicates that the red deer's current genetic background has presumably retained much of the genetic variation present in those relict populations. We also found that a substantial portion (32%) of the populations displays some degree of inbreeding. We suggest that new herds should be established using individuals from the different genetic clusters, and a careful monitoring of the breeder's genetic background to prevent further inbreeding and inadvertent hybridization. Failure to do so could lead to loss of genetic diversity and the dilution of the genetic identity of the Iberian red deer.Peer Reviewe

Demographic trends of a reintroduced Iberian ibex Capra pyrenaica victoriae population in Central Spain

One reintroduced population of Iberian ibex was monitored between 2000 and 2007 in the Sierra de Guadarrama National Park (Central Spain) using the distance sampling method. The densities obtained from three samplings show a significant increase between 2000 (6.57 ind./km2 ) and 2007 (33.16 ind./km2 ) despite a range extension. After an initial period of balance, the sex ratio became unbalanced over time in favor of females. The age pyramid also changed after a dramatic population increase. The birth rate oscillated at relatively high values for the species (0.69–0.99 kids/female). The group size reduced significantly over time. Females with kids dispersed from the release area significantly less than males and mixed groups

Negative effect of the arthropod parasite, Sarcoptes scabiei, on testes mass in Iberian ibex, Capra pyrenaica

Testes mass is a key factor in male reproductive success and is potentially exposed to so-called 'parasitic castration'. This is the result of the direct destruction or alteration of reproductive cell lineages (parasitic castration sensu stricto), or the indirect detrimental effects - for example, via body condition - on the ability of progenitors to produce or rear offspring (parasitic castration sensu lato). There are enormous gaps in our knowledge on the effects of parasites on the testes of wild mammals and in an attempt to rectify this dearth of data we examined the relationship between the skin parasite Sarcoptes scabiei and testes mass in Iberian ibex Capra pyrenaica. We considered data from 222 males that were culled in the population from the Sierra Nevada in Spain. Our results provide evidence that sarcoptic mange is associated with reduced size-corrected testes mass in Iberian ibex which supports the hypothesis that parasitism is a determining factor in gonad plasticity in male mammals. We discuss several hypothetical causes of this relationship and highlight the need to deepen the sub-lethal effects of pathogens if we are to accurately understand their modulator effects on host population dynamics. © 2010 Elsevier B.V.Peer Reviewe

How sensitive and specific is the visual diagnosis of sarcoptic mange in free-ranging Iberian ibexes?

Background: Sarcoptic mange is a broadly distributed parasitic disease caused by Sarcoptes scabiei that affects wild mammals from all over the world, including the Iberian ibex (Capra pyrenaica). Selective culling of the scabietic individuals is the main management measure for disease control in Iberian ibex populations. Although visual identification of mange-compatible lesions is the reference method to decide the target individual, both false negative and positive cases are common in the wild. The aim of this work is to determine the sensitivity (SE), and the specificity (SP) of selective culling after evaluating 403 ibexes hunted in the Sierra Nevada Nature Space for sarcoptic mange control between 2002 and 2015. Methods: A combination of skin scrapings and potassium hidroxide (KOH) skin digestion was used for sarcoptic mange diagnosis. Generalized linear models (GLM) were used to assess the effects of sex, age (juveniles and adults) and period of the year (wet and dry periods) on the SE and SP of the visual diagnosis method. Results: The SE obtained for the visual determination of scabietic ibexes was 87.14%, whereas the SP was 60.71%. According to our model selection, SE of the visual diagnosis was explained by the additive effects of age and the period of the year. In fact, SE was lower in juveniles (64.76%) than in adults (94.26%) and during the dry period (73.44%) as compared to the wet period (92.09%). On the other hand, SP was best explained by the GLM including the additive effects of sex and the period of the year. The visual diagnosis of sarcoptic mange resulted less specific in females (22.73%) than in males (74.19%) and during the wet (55.22%) than in the dry period (82.35%). Conclusions: Maximizing SE and SP is essential to achieving a high rate of removal of affected individuals from the environment without eliminating potentially resistant individuals. Selective culling must be conservative during the wet period and with females due to the lower SP. Conversely, visual diagnosis of scabietic juveniles and during the dry period has to be improved, due to the lower SE.This project was funded by the Consejería de Medio Ambiente de la Junta de Andalucía (project 173/2009/M/00; 03/15/M/00; 861_11_M_00 and 2016/00014/M), and by the Spanish Ministerio de Economía y Competitividad (projects CGL2012-40043-C02-01, CGL2012-40043-C02-02 and CGL2016-80543-P). The authors’ research activities are partially supported by the Plan Andaluz de Investigación (RNM-118 group). MV is supported by a FI-GENCAT Fellowship (2018FI_B_00978, co-financed by Agència de Gestió dʼAjuts Universitaris i de Recerca and European Social Fund) and ES by the Spanish Ministerio de Ciencia Innovación y Universidades (MICINN) through a Ramon y Cajal agreement (RYC-2016-21120)

El ciervo en Andalucía

El ciervo o venado —término más cinegético— se asienta principalmente en el arco montañoso, de altitud media y de pendiente suave, que recorre Andalucía de noreste a sureste, desde la provincia de Jaén hasta la de Huelva, pasando por Córdoba y Sevilla. Se denomina Sierra Morena a este accidente orográfico y el nombre, en la eti- mología más común, parece responder a su color característico. La vocación de esta sierra históricamente y, en la actualidad, es agrosilvocinegéti- ca. Los tres elementos de esta palabra compuesta han oscilado en su proporción a lo largo del tiempo, según la coyuntura, y en función de las características edáficas —las climáticas son más uniformes— se reparten los aprovechamientos en un mosaico no excesivamente complejo. El paisaje, a veces, se abre en valles, el más típico y amplio el de Los Pedroches, donde la componente agrícola y ganadera es el aprovechamiento casi exclusivo hasta el día de hoy, en el que la coyuntura comienza a cambiar, y se inicia la reforestación que significará un cambio acaso importante, y el principio del regreso a los orígenes. Si el monte aumenta su densidad, y sobre todo la mancha de matorral se cierra y ocupa áreas significativas, entonces la vocación o especialización prioritaria es la cinegética. El hábitat es, sin duda, idóneo y apropiado para que se asiente y desarrolle el ciervo, junto con otras especies, como puede ser el jabalí, el gamo y la cabra mon- tés y doméstica, o aún el corzo en su nicho natural de la Sierra de Cádiz. Lo dicho es característico en conocidas zonas de Jaén, Córdoba y Sevilla. En otras ocasiones, el hábitat natural ha sido transformado artificialmente por plantaciones de pinos y de eucaliptus, particularmente en Huelva y en áreas puntuales de las otras provincias. El aprovechamiento selvícola artificial e intensivo dificulta el desarrollo de la cubierta vegetal y, por ello, no permite que las especies cinegéticas se asienten de modo permanente si no existen zonas próximas del típico hábitat y monte mediterráneo

The local skin cellular immune response determines the clinical outcome of sarcoptic mange in Iberian ibex (Capra pyrenaica)

Sarcoptic mange, caused by Sarcoptes scabiei, is a disease with implications for wildlife conservation and management. Its severity depends on the host's local skin immune response, which is largely unknown in Iberian ibex (Capra pyrenaica), a mountain ungulate dramatically affected by mange. In this species, the clinical outcome of sarcoptic mange varies among individuals, and the local immune response could be key to controlling the infestation. This study aims to characterize the local cellular immune response and its relationship with the clinical outcome. Fourteen Iberian ibexes were experimentally infested with S. scabiei and six more served as controls. Clinical signs were monitored, and skin biopsies were collected from the withers at 26, 46, and 103 days post-infection (dpi). The presence and distribution of macrophages (including M1 and M2 phenotypes), T lymphocytes, B lymphocytes, plasma cells, and interleukine 10 were quantitatively evaluated using immunohistochemical techniques. An inflammatory infiltrate that decreased significantly from 26 to 103 dpi was observed in all the infested ibexes. The predominant inflammatory cell population in the skin of the mangy ibexes was formed by macrophages (mainly the M2 phenotype) followed by T lymphocytes, with lower numbers of B lymphocytes and plasma cells. Three clinical courses were identified: total recovery, partial recovery, and terminal stage. The inflammatory infiltrates were less pronounced in the fully recovered ibexes than in those that progressed to the terminal stage throughout the study. The results suggest an exacerbated but effective Th1-type cellular immune response controlling mange in Iberian ibex. Furthermore, the local immune response appears to determine the variability of the clinical responses to S. scabiei infestation in this species. This first report on the progression of local skin immune cells is relevant not only for individuals but also for population management and conservation

The local skin cellular immune response determines the clinical outcome of sarcoptic mange in Iberian ibex (Capra pyrenaica)

[EN] Introduction: Sarcoptic mange, caused by Sarcoptes scabiei, is a disease with implications for wildlife conservation and management. Its severity depends on the host’s local skin immune response, which is largely unknown in Iberian ibex (Capra pyrenaica), a mountain ungulate dramatically affected by mange. In this species, the clinical outcome of sarcoptic mange varies among individuals, and the local immune response could be key to controlling the infestation. This study aims to characterize the local cellular immune response and its relationship with the clinical outcome. Methods: Fourteen Iberian ibexes were experimentally infested with S. scabiei and six more served as controls. Clinical signs were monitored, and skin biopsies were collected from the withers at 26, 46, and 103 days post-infection (dpi). The presence and distribution of macrophages (including M1 and M2 phenotypes), T lymphocytes, B lymphocytes, plasma cells, and interleukine 10 were quantitatively evaluated using immunohistochemical techniques. Results: An inflammatory infiltrate that decreased significantly from 26 to 103 dpi was observed in all the infested ibexes. The predominant inflammatory cell population in the skin of the mangy ibexes was formed by macrophages (mainly the M2 phenotype) followed by T lymphocytes, with lower numbers of B lymphocytes and plasma cells. Three clinical courses were identified: total recovery, partial recovery, and terminal stage. The inflammatory infiltrates were less pronounced in the fully recovered ibexes than in those that progressed to the terminal stage throughout the study. Discussion: The results suggest an exacerbated but effective Th1-type cellular immune response controlling mange in Iberian ibex. Furthermore, the local immune response appears to determine the variability of the clinical responses to S. scabiei infestation in this species. This first report on the progression of local skin immune cells is relevant not only for individuals but also for population management and conservation.SIThis project was funded by the Consejería de Medio Ambiente de la Junta de Andalucía (project 173/2009/M/00;03/15/M/00; 861_11_M_00 and 2016/00014/M) and the Spanish Ministerio de Economía y Competitividad (projects CGL2012-40043-C02-01, CGL2012-40043-C02-02, and CGL2016-80543-P). The authors’ research activities are partially supported by the Plan Andaluz de Investigación (RNM-118 group). MV is supported by a FI-GENCAT Fellowship (2020_FI_B2_00049, which is cofinanced by the Agència de Gestió d’Ajuts Universitaris i de Recerca and the European Social Fund). GM is a Serra Húnter FellowThe authors would like to thank the Consejería de Medio Ambiente de la Junta de Andalucía and in particular the Sierra Nevada Natural Space for their logistical support and the Sierra de Huétor Natural Park for the transfer of their experimental facilities. We also give special thanks to the park wardens and fieldworkers in the SNNS and above all to Apolo Sánchez, José López, Isidro Puga, Elías Martínez, Francisco Felipe, and Antonio Rodríguez for their professional and personal involvement in the study. The authors are also grateful to Manolo Herrera for the maintenance of the ibex and the facilities during the experimental phase, Emmanuel Serrano for advice, and the Service of Veterinary Pathology of the UAB for the histological preparation of samples. The technical and human support provided by Marta Silva is gratefully acknowledged