原条から体節中胚葉へ : 初期発生段階のニワトリ胚のEGFP遺伝子導入による標識

内容の要旨 , 審査の要旨広島大学(Hiroshima University)博士(医学)Doctor of Philosophy in Medical Sciencedoctora

MAKET KRATON PLERED CIRCA : 1675

Maket ini berusaha mengangkat suatu program yang subjeknya cukup menantang yakni, Kraton Plered yang keberadaannya sekarang sudah hancur rata dengan tanah. Pertimbangan kurikuler historis didaktis tetap menjadi acuan seleksi subjek model dan dilakukan secara objektif saintifik, Tentatif Historis Imajinasi Berdasarkan Kesaksian Sejarah Tujuan Penelitian ini adalah sebuah analisis historis pertumbuhan dan perkembangan Kraton Plered serta merupakan visualisasi Kraton Plered beserta lingkungan sekitarnya pada masa Kasultanan Amangkurat Agung (I). Penelitian ini menggunakan metode sejarah yakni, diawali dari penentuan subjek, perumusan problema, survai, pengumpulan sumber, analisa sumber, kesimpulan sementara dan berakhir dengan seminar umum, follow up hasil seminar, disimpulkan dalam bentuk grafis (peta, diagram, Chart, denah, irisan, tampak depan, samping, dan atas) "BLUEPRINT". Berdasarkan blueprint, kerja fisik pembuatan maket dilakukan, yang meliputi langkah memotong, merakit, memasang dan finishing. Hasil penelitian merupakan rekonstruksi sebuah model tiga dimensi berupa maket Kraton Plered beserta kelengkapannya, seperti ilustrasi, fotografi, poster, peta, makalah, bibliografi, dokumen, audio visual, dan sebagainya. Kesemuanya untuk sementara disimpan di Ruang Disply Laboratorium Sejarah, FKIP Unversitas PGRI Yogyakarta, Kampus Unit I lantai I gedung B

Complex Precipitation Behavior in a Co-Free High Entropy Alloy during Aging

High entropy alloys (HEAs) demonstrate high strength, thermal stability, and irradiation resistance, making them desirable for applications in nuclear reactors and other harsh environments. Many existing HEAs contain cobalt (Co), which makes them unsuitable for nuclear applications due to the long-term activation of Co. A previously studied Co-free alloy, (Fe0.3Ni0.3Mn0.3Cr0.1)88Ti4Al8, exhibits high strength but compromised ductility due to its network of brittle precipitates upon aging. This work investigates the formation mechanism and evolution of the precipitates, including L12 (Ni3Ti type, ordered face-centered cubic structure), B2 (NiAl type, ordered body-centered cubic structure), and Chi (FeCr-rich ordered α-Mn structure), in this alloy at 650 °C, the aging temperature that yields the alloy\u27s peak strength after 120 hours. Using ex-situ scanning electron microscopy, transmission electron microscopy, and atom probe tomography, the precipitates were characterized in terms of their composition, morphology, and crystal structure at various aging times. In addition, synchrotron-based, in-situ X-ray diffraction was used to probe the evolution of precipitates in the bulk during key phases of in-situ aging. The L12 precipitates exhibit complex growth and coarsening behavior – their coarsening starts after just 24 hours, and competition between the L12 and B2 phases arises after 72 hours leading to a decrease in L12 volume fraction. Conversely, B2 and Chi precipitates exhibit delayed nucleation and growth, and do not form in observable quantities until after 24 hours. Once formed, however, these precipitates quickly dominate the microstructure, with \u3e50 % volume fraction after 120 hours, leading to the previously observed mechanical properties. This study provides a foundational understanding of the evolution of multi-precipitate structures in HEAs and guides future alloy development and optimization by tailoring the precipitates

Strain Rockbursts Simulated by Low-Strength Brittle Equivalent Materials

This paper presents experimental study on rockbursts that occur in deep underground excavations. To begin with, the boundary conditions for excavation in deep underground engineering were analysed and elastic adaptive boundary is an effective way to minimize the boundary effect of geomechanical model test. Then, in order to simulate an elastic adaptive loading boundary, Belleville springs were used to establish this loading boundary. With the aforementioned experimental set-ups and fabrication of similarity models for test, the phenomena of strain mode rockbursts were satisfactorily reproduced in laboratory. The internal stress, strain, and convergences of the openings of the model were instrumented by subtly preembedded sensors and transducers. Test results showed that, with an initial state of high stress from both upper layers’ gravitational effects and in situ stress due to tectonic movements, the excavation brings a dramatic rise in the hoop stress and sharp drop in radial stress, which leads to the splitting failure of rock mass. Finally a rockburst occurred associated with the release of strain energy stored in highly stressed rock mass. In addition, the failure of the surrounding rock demonstrated an obvious hysteresis effect which supplies valuable guide and reference for tunnel support. Not only do these results provide a basis for further comprehensive experiments, but also the data can offer assisting aids for further theoretical study of rockbursts

Human-like collagen protein-coated magnetic nanoparticles with high magnetic hyperthermia performance and improved biocompatibility

Human-like collagen (HLC)-coated monodispersed superparamagnetic Fe(3)O(4) nanoparticles have been successfully prepared to investigate its effect on heat induction property and cell toxicity. After coating of HLC, the sample shows a faster rate of temperature increase under an alternating magnetic field although it has a reduced saturation magnetization. This is most probably a result of the effective heat conduction and good colloid stability due to the high charge of HLC on the surface. In addition, compared with Fe(3)O(4) nanoparticles before coating with HLC, HLC-coated Fe(3)O(4) nanoparticles do not induce notable cytotoxic effect at higher concentration which indicates that HLC-coated Fe(3)O(4) nanoparticles has improved biocompatibility. Our results clearly show that Fe(3)O(4) nanoparticles after coating with HLC not only possess effective heat induction for cancer treatment but also have improved biocompatibility for biomedicine applications

Label-free visualization of carbapenemase activity in living bacteria

Evaluating enzyme activity intracellularly on natural substrates is a significant experimental challenge in biomedical research. We report a label‐free method for real‐time monitoring of the catalytic behavior of class A, B, and D carbapenemases in live bacteria based on measurement of heat changes. By this means, novel biphasic kinetics for class D OXA‐48 with imipenem as substrate is revealed, providing a new approach to detect OXA‐48‐like producers. This in‐cell calorimetry approach offers major advantages in the rapid screening (10 min) of carbapenemase‐producing Enterobacteriaceae from 142 clinical bacterial isolates, with superior sensitivity (97 %) and excellent specificity (100 %) compared to conventional methods. As a general, label‐free method for the study of living cells, this protocol has potential for application to a wider range and variety of cellular components and physiological processes

Endoscopic variceal ligation combined with carvedilol versus endoscopic variceal ligation combined with propranolol for the treatment of oesophageal variceal bleeding in cirrhosis: study protocol for a multicentre, randomised controlled trial

Introduction Liver cirrhosis and its severe complication, oesophageal variceal bleeding (EVB), pose significant health risks. Standard treatment for EVB combines non-selective beta-blockers (NSBB) with endoscopic variceal ligation (EVL). Carvedilol, an NSBB with additional benefits, is preferred for compensated cirrhosis. However, no randomised controlled trial (RCT) has compared carvedilol with propranolol, a conventional NSBB, in combination with EVL for secondary prophylaxis. This study aims to compare the effectiveness and safety of these treatments in preventing variceal rebleeding or death in patients with cirrhosis and EVB. Methods and analysis This multicentre, RCT is scheduled to begin in December 2024, with recruitment and follow-up continuing until December 2026. Eligible participants are patients with liver cirrhosis and EVB. Participants are randomly assigned in a 1:1 ratio to receive EVL combined with either carvedilol or propranolol. The primary endpoint is the incidence of variceal rebleeding or all-cause death. Secondary endpoints include all-cause death, liver-related death, each of the complications of portal hypertension (overt ascites, overt hepatic encephalopathy, spontaneous bacterial peritonitis, hepatorenal syndrome, portal vein thrombosis), hepatocellular carcinoma, changes in liver function (assessed by Child-Pugh and Model for End-Stage Liver Disease scores), changes in liver stiffness, changes in spleen stiffness, and adverse events. Subgroup and sensitivity analyses will be conducted to evaluate the consistency and robustness of the treatment effects. A total sample size of 524 patients (262 per group) is required to detect a significant difference between the treatment arms. Ethics and dissemination The study protocol has been approved by the ethics committee of the First Hospital of China Medical University (No. 2024-656-2). The study will follow the Declaration of Helsinki and Good Clinical Practice guidelines. The findings of this trial will be disseminated through peer-reviewed publications, conference presentations and healthcare professionals to guide future clinical practice