Creation and promotion Iranian fisheries research institute laboratories database

Creating a database of affiliated laboratories of the institute is organized with the aim of integrating information related to laboratories of research centers and their subsidiaries. The main objective of conducting this project in this stage is to upgrade it, establish and run one software system based on up-to-date networking technology. For this purpose organizing the centers database, a periodic report on various aspects can be done which help for implementing appropriate monitoring and management. Among the sectors that are designed and upgraded for this system include: Portal, information bank, advanced possibilities for inputting data, searching and reporting on laboratory. The advantages of this precise and updated reports can be collected easily from the general information of research institutes and centers, reports of the number of lab experts with different educational levels in affiliated centers, awareness of numbers and status of the chemical materials in the laboratories of each center, and the significant and important point is about economizing equipment, chemical materials and on time calibration

Effect of flavonoids of extract of cichorium intybus L. leaf on induction of P19 stem-cells differentiation to insulin-producing cells

Background and purpose: Regarding the effective role of plant flavonoids in the prevention and treatment of diabetes mellitus, in this investigation, induction of P19 stem cells differentiation into insulin producing cells by flavonoids of methanol extract of Cichorium intybus L. leaf was examined. Materials and methods: Initially, flavonoids of methanol extract of Cichorium intybus L. leaf were concentrated. The P19-derived embryonic bodies were cultured in α-MEM (Minimum Essential Medium) containing 15 fetal bovine serum for three days. To induce the differentiation, the cells treated with the concentrations of 50, 100 and 200 μg/ml of flavonoids of methanol extract of Cichorium intybus L. leaf for 12 days. Untreated P19 cells and embryonic bodies were used as controls. Dithizone staining, immunoflorecence and real-time polymerase chain reaction (RT-PCR) techniques were used to demonstrate the differentiated cells. Results: Differentiated P19 cells by different concentrations of extract shown positive reaction to dithizone staining. Most percentage of positive dithizone cells was reached in concentration of 100 μg/ml. Immunoflorecence method showed that differentiated P19 cells were able to express the pancreas beta cell-specific markers. Pdx-1 gene expression in the cells was demonstrated by RT-PCR technique. Conclusion: The flavonoids of methanol extract of Cichorium intybus L. leaf are able to induce differentiation of P19 stem cells to insulin producing cells. © 2014 Mazandaran University of Medical Sciences. All rights reserved

Simultaneous determination of irinotecan hydrochloride and its related compounds by high performance liquid chromatography using ultraviolet detection

A new simple, precise and accurate high performance liquid chromatography (HPLC) method was developed and validated for the simultaneous determination of irinotecan (CPT-11) and two related compounds viz., 7-ethyl-10 hydroxycamptothecin (SN-38) and camptothecin (CPT) in pharmaceutical dosage forms. Chromatography was accomplished using a reversed-phase C18 column and ultraviolet (UV)detection and an isocratic mobile phase consisting of 3 % v/v triethylammonium acetate buffer (pH 3) and acetonitrile (70:30 v/v). The linear range of quantitation for all the compounds was 0.1-10 μg/mL. The limit of quantitation for all the compounds ranged between 0.01-0.05 μg/mL. The method has the requisite accuracy, selectivity, sensitivity and precision to assay of CPT-11 and related compounds in pharmaceutical dosage forms and bulk API

Differentiation of embryonal carcinoma stem cells into insulin-producing cells by using pancreas extract in vitro

Introduction: Type I diabetes mellitus results from the autoimmune destruction of the β cells in pancreatic islets. Currently, extensive research is being conducted on the generation of insulin-producing cells (IPCs) from stem cells. P19 embryonal carcinoma cells are multipotent and can differentiate into cell types of all three germ layers. In this study, the differentiation of P19 cells into IPCs by using mouse pancreas extract (MPE) was investigated. Materials and Methods: Embryoid bodies (EBs) obtained from P19 cells were cultured in medium containing 3 fetal bovine serum, supplemented by concentration of 50, 100, 200,300 μg/mL MPE for 7-14 days. Dithizone (DTZ) staining was used to detect IPCs derived from EBs in vitro. Mouse monoclonal insulin-proinsulin and monoclonal insulin receptor beta antibodies were used for immunoflourescence. Insulin content from the cells and insulin secreted by differentiated cells in response to concentrations of 5.5 and 25 mM glucose were measured using ELISA kits. Results: DTZ-positive cells showed purple-red clusters. immunoflourescence indicated expression of Beta cell markers (insulin-proinsulin and insulin receptor beta) in these cells. Increasing glucose concentration, caused more insulin to be secreted by differentiated cells. Conclusions: P19 cells can in the presence of pancreas extract differentiate to cell producing and secreting insulin cells. Differentiated cells can increase insulin secretion in response to increasing glucose medium

Isolation and identification of Flavobacterium columnaris like organisms from grass carp (Ctenopharyngodon idella) and assessment of its histopathological effects in Khouzestan Province, Southern Iran

Following a mortality of up to 40% of cultured Grass Carp (Ctenopharyngodon idella) in fish farms of Khouzestan province when water temperature was up to 20°C, samples were taken and examined for etiological clues. We observed that fish gills were coated with a gray-white mucus layer, fin was eroded and wounds were present in peduncle of the affected samples. Bacteriological studies on the samples of gills and eroded and wounded peduncle skin using Cytophaga selective medium, resulted in isolation of filamentous gram negative bacteria chemically similar to Flavobacterium columnaris. Histological observations showed the affected fish carrying symptoms including congestion, hemorrhagia, edema in base membrane, hyperplasia of chondrocyte and secondary lamellae cells, fusion of lamellae, necrosis and peeling of secondary lamellae and also presence of filamentous bacteria in gill tissue sections. Necrosis of skin layers and expansion of ulcer to underlying muscles, degeneration of muscle cells and necrosis of muscle bundles were seen in peduncle muscles. We did not find this bacterium in internal organs. We conclude that the isolation of the bacterium and observation of histo-pathological changes suggest the organisms may be considered as a primary or secondary factor in occurrence of the disease

Effect of pancreatic extract on insulin secreting cell differentiation from mouse bone marrow mesenchymal stem cells

زمینه و هدف: با دیابت نوع یک در نتیجه ی تخریب خود ایمنی سلول های بتای جزایر پانکراس ایجاد می شود. مطالعات اخیر نشان می‌دهد بسیاری از انواع سلول های بنیادی می توانند به عنوان منابع احتمالی برای به دست آوردن سلول های قابل پیوند تولید کننده انسولین (IPCs) در نظر گرفته شوند. در این مطالعه تمایز سلول های بنیادی مزانشیمی مغز استخوان به سلول های مولد انسولین با استفاده از عصاره پانکراس موش مورد بررسی قرار گرفت. روش بررسی: در این مطالعه تجربی آزمایشگاهی از سلول های بنیادی مزانشیمی مغز استخوان موش کوچک آزمایشگاهی برای تمایز به سلول های انسولین ساز استفاده شد. سلول های تمایز یافته با استفاده از رنگ اختصاصی دیتیزون و آنتی بادی های ضد انسولین- پروانسولین و ضد رسپتور بتای انسولین مورد ارزیابی قرار گرفتند. همچنین بیان ژن اختصاصی سلول های پانکراسی یعنی pdx-I در این سلول ها با روش RT-PCR بررسی شد. یافته ها: سلول های تمایز یافته مشتق از روش مستقیم مورفولوژی مشابه با سلول های بتای پانکراس نشان دادند. سلول های دیتیزون مثبت به صورت دستجات قرمز ارغوانی دیده شدند. نتایج بررسی RT-PCR بیان ژن اختصاصی سلول های بتا (pdx-I) را در سلول های تمایز مستقیم نشان داد. ایمنوفلورسنس وجود نشانگرهای اختصاصی سلول های بتا را دراین سلول ها به اثبات رساند. نتیجه گیری: نتایج این مطالعه نشان داد که سلول های بنیادی مزانشیمی مغز استخوان می توانند در حضور عصاره پانکراس به سلول های مولد انسولین تمایز یابند؛ لذا استفاده از این نتایج تولید سلول های بتا از سلول های بنیادی در شرایط آزمایشگاهی را تسهیل می کند

Evaluation of pdx-1 gene expression in insulin producing cells, derived from embryonal carcinoma stem cells

زمینه و هدف: درک عملکرد سلول های بتا در سطح مولکولی به احتمال زیاد توسعه تکنیک های تولید سلول های بتا را تسهیل می کند. این مطالعه با هدف بررسی بیان ژن هومئوباکس دئودنال 1 (pdx-1) در سلول‌های تمایز یافته طراحی و اجرا شد. روش بررسی: این مطالعه بنیادی-کاربردی بر روی تمایز سلول‌های بنیادی به سلول‌های انسولین‌ساز انجام گرفت. محیط ثانویه حاصل از کشت پانکراس نوزاد یک هفته‌ای موش برای تمایز سلول‌های P19 استفاده شد. اجسام شبه جنینی (EBs) با کشت معلق 24 ساعته سلول‌های P19 تشکیل شدند. برای القای تمایز، غلظت‌های متفاوت محیط ثانویه (25، 50، 75 و 100) به محیط کشت اضافه شد. جهت شناسایی سلول‌های تمایز یافته مشتق از EBs در شرایط آزمایشــگاهی از رنگ آمیزی دیتیزون استفاده شد. تولید انسولین-پروانسولین و رسپتور بتای انسولین در این سلول ها به روش ایمنوفلورسنس تعیین و بیان ژن pdx-1 به وسیله واکنش زنجیره پلی‌مراز-رونویسی معکوس ارزیابی شد. داده ها با استفاده از آزمون های آماری آنالیز واریانس یک طرفه و دانکن تجزیه و تحلیل شدند. یافته‌ها: پس از هفت روز القا، دستجات سلولی تمایز یافته ظاهر شدند. اوج پاسخ‌گویی تمایزی مربوط به غلظت 50 از محیط ثانویه بود. بیان ژن pdx-1 در دستجات سلولی تمایز یافته مشاهده شد. بیان نشانگرهای انسولین-پروانسولین و رسپتور بتا در سلول‌های تمایز یافته به روش ایمنوفلورسنس اثبات شد. نتیجه‌گیری: محیط ثانویه پانکراس باعث تمایز سلول‌های P19به سلول‌های انسولین ساز ‌‌شد، لذا نتایج این مطالعه می تواند تولید سلول های بتا را از سلول های بنیادی تسهیل نماید

Suffering Sources among the Newly-Graduated Nurses at the Beginning of Their Clinical Work: A Qualitative Study

Suffering is an inevitable reality at the beginning of work that may cause several damages to the amateur nurses and health care organizations. Although suffering has been investigated in some studies, its sources among the newly-graduated nurses have not been investigated so far. The goal of this study is to investigate suffering sources of the newly-graduated nurses at the beginning of work. The present research is a qualitative content analysis study. The participants included 17 amateur nurses of Tehran educational hospitals. The data was collected through a semi-systematic interview. Sampling was made by using a targeted method and was continued until data saturation. All interviews were recorded and were then written down and were analyzed by using the qualitative content analysis method. Findings of this study showed that suffering is a joint experience among the newly-graduated nurses at the first months of their beginning to work. The findings showed the suffering sources among the nurses. Four main themes emerged in this study, namely non-preparedness for working, workplace, patients, and colleagues as the suffering sources. Experiences of the participants showed that suffering of the newly-employed nurses has extensive sources that affect their personal and professional lives. Understanding suffering sources of the newly-employed nurses can be an important factor in helping this group of nurses. Sensitivity of nursing managers to the reduction of these suffering sources and supporting the newly-graduated nurses are very important. Rafii F, Sajadi hezaveh M, Seyedfatemi N, Esmaeili Vardanjani SA. Suffering Sources among the Newly-Graduated Nurses at the Beginning of Their Clinical Work: A Qualitative Study. Life Sci J 2012;9(4):5517-5524] (ISSN:1097-8135). http://www.lifesciencesite.com. 81

The fluctuations of physicochemical factors and phytoplankton populations of Urmia Lake, Iran

Urmia Lake is one of the two large hypersaline lakes in the world which have Artemia. It is located in northwest of Iran. Due to a decrease in water inflow and volume, the salinity of Urmia Lake has reached to more than 300 g.l-1 since 2001. The increased salinity has greatly influenced biological aspects of the lake, and caused the lake undergoes at critical conduction. The aim of the present study was to investigate the distribution fluctuations of phytoplanktons and selected physicochemical factors in relation to Artemia distribution in Urmia Lake during 8 months. Statistical analysis of mean values of ion concentrations and phytoplankton abundance indicated significant differences among sampling months. The minimum and maximum values for the selected factors were, as Cl- 176.2-201.3 g.l-1 , CO2 95-175mg.l-1 , dissolved oxygen (DO) 0.1-2.8 mg.l-1 , HCO3 - 144-496 mg.l-1 , PO42+ 104-875 mg.l-1, NO3- 330-4104 mg.l-1, NO2- 4-21.5 mg.l-1, SO42- 10490-29840 mg.l-1, Ca2+ 561-1606 mg.l-1, Mg2+ 3649-14587 mg.l-1 while water hardness was 21000- 62000 mg.l-1. Fourteen phytoplankton genera included Bacillariophyceae (10 genera),Chlorophyceae (2 genera) and Cyanophyceae (2 genera) were identified during sampling period. The smallest average density of phytoplankton 97249 L-1 was observed in December 2005 and the greatest average density 481983 L-1 in August 2005. Dunaliella sp. composed 92.1% of the lake's phytoplankton. Statistical analysis of phytoplanktons fluctuations showed a significant difference among different months (p< 0.05)

Surveying and Comparing the effect of Two Training Methods: Drug Addiction Prevention (Peer education with Teachers) on the Level of Knowledge and Attitudes on the High School Students of ShahreKord, Iran

Considering the increasing growth of addiction in the youth as well as the importance of training to avoid that, the effect of two training methods (by the teacher and peer education) for preventing from the addiction of high school boy students of Shahr-e-Kord were studied in this research. In this study 450 boy students in third grade at high school were included in the study from Shahr-e-Kord High Schools on a random and stratified basis. 225 of the total 450 studied students were trained by the teacher and 225 students were trained by the group of the same age. 20 days after completion of training, a posttest was made by using the same questionnaire. The collected data was analyzed by spss 11.5 and by using descriptive and analytical statistics. The studied students included 450 students with an average age of 16.78 +/- 0.7. Average pretest and posttest scores in both interventional groups were significantly different (p=/0001). Average change of pretest and posttest scores in the training group by the students of the same age (10/.7 +/- 3.6) was higher than the average change of scores before and after the training by the teacher (8.8 +/- 3.4). Bon Froni test showed these differences to be statistically significant (p=0.0001). Considering the findings of this study, training on how to avoid addiction by those of the same age will be more efficient in increasing the knowledge and views of the students. Baraz S, Rostami M, Karimi Pour F, Lalehgani H, Esmaeili Vardanjani SA. Surveying and Comparing the effect of Two Training Methods: Drug Addiction Prevention (Peer education with Teachers) on the Level of Knowledge and Attitudes of the High School Boy Students of Shahr-e-Kord, Iran. Life Sci J 2012;9(2):1304-1307] (ISSN: 1097-8135). http://www.lifesciencesite.com. 19