2 research outputs found
Rapid Sample Enrichment, Novel Derivatization, and High Sensitivity for Determination of 3‑Chloropropane-1,2-diol in Soy Sauce via High-Performance Liquid Chromatography–Tandem Mass Spectrometry
A novel, simplified derivatization method and a rapid
sample preparation
process using carbon yarn as a sorbent for the determination of 3-chloropropane-1,2-diol
(3-MCPD) in soy sauce via high-performance liquid chromatography–tandem
mass spectrometry (HPLC-MS/MS) was developed. 3-MCPD was first enriched
and purified with carbon yarn and then eluted with a methanol–water
solution. Subsequently, the analyte underwent derivatization with p-(dimethylamino)-phenol for sensitive detection via HPLC-MS/MS.
The limit of detection and the limit of quantitation for 3-MCPD were
validated to be 0.5 and 1.0 μg/kg, respectively. Spiking experiments
showed recoveries between 83 and 94%, with a relative standard deviation
of ≤10%. The method was further validated with a certified
reference material. Furthermore, 11 real soy sauce samples from local
markets were tested by using this method. These results reveal the
widespread 3-MCPD contamination. Consequently, this study offers a
preferable alternative for the sensitive, accurate, and precise determination
of 3-MCPD in soy sauce
Dual-Modal Apoptosis Assay Enabling Dynamic Visualization of ATP and Reactive Oxygen Species in Living Cells
ATP and reactive oxygen species (ROS) are considered
significant
indicators of cell apoptosis. However, visualizing the interplay between
apoptosis-related ATP and ROS is challenging. Herein, we developed
a metal–organic framework (MOF)-based nanoprobe for an apoptosis
assay using duplex imaging of cellular ATP and ROS. The nanoprobe
was fabricated through controlled encapsulation of gold nanorods with
a thin zirconium-based MOF layer, followed by modification of the
ROS-responsive molecules 2-mercaptohydroquinone and 6-carboxyfluorescein-labeled
ATP aptamer. The nanoprobe enables ATP and ROS visualization via fluorescence
and surface-enhanced Raman spectroscopy, respectively, avoiding the
mutual interference that often occurs in single-mode methods. Moreover,
the dual-modal assay effectively showed dynamic imaging of ATP and
ROS in cancer cells treated with various drugs, revealing their apoptosis-related
pathways and interactions that differ from those under normal conditions.
This study provides a method for studying the relationship between
energy metabolism and redox homeostasis in cell apoptosis processes