16 research outputs found

    Analyses of the detergent-insoluble membrane proteins of chloramphenicol-resistant variants.

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    <p>SDS-PAGE analysis was performed on ATCC700388 and PHLSE082, two wild-type strains of <i>B. thailandensis</i>, and on 64CM16 and 132CM128, their respectively chloramphenicol resistant derivative strains. Proteins were stained with Coomassie blue. The variants presented different additional bands at around 51 kDa (band A), 48 kDa (band B), 43 kDa (bands C and band E) and at 95 kDa (band D). Molecular weight standards are indicated in kilodaltons.</p

    Antibiotic susceptibility of the <i>B. thailandensis</i> strains.

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    <p>Antimicrobial agent abbreviations: CM, chloramphenicol; NAL, nalidixic acid; NFX, norfloxacin; CIP, ciprofloxacin; FOX, cefoxitin; CAZ, ceftazidime; OXA, oxacillin; CLO, cloxacillin; PIP, piperacillin; IMI, imipenem; ERY, erythromycin; TC, tetracycline; DC, doxycycline; TP, trimethoprim; TS, trimethoprim/sulfamethoxazole; POL B, polymyxin B. Values are means of three independent determinations.</p

    Susceptibility of <i>B. thailandensis</i> strains to Ăź-lactams and aminoglycosides.

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    <p>Antimicrobial agent abbreviations: AMX, amoxicillin; AMC, amoxicillin/clavulanate (2/1); OXA, oxacillin; TIM, ticarcillin/clavulanic acid (2 mg/L); TMO, temocillin; PIP, piperacillin; TZP, piperacillin/tazobactam (4 mg/L); CEF, cefalotin; CXM, cefuroxime; CAZ, ceftazidime; CTX, cefotaxime; ATM, aztreonam; IPM, imipenem; ETP, ertapenem; MEM, meropenem; DPM, doripenem; AMK, amikacin; GEN, gentamicin; TOB, tobramycin; KAN, kanamycin; NET, netilmicin.</p

    Effects of PAĂźN on the antibiotic susceptibility of <i>B. thailandensis</i> strains and mutants.

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    <p>Abbreviations: PAßN, phenylalanine-arginine ß-naphthylamide; DOX, doxycycline; CHL, chloramphenicol; NAL, nalidixic acid; SXT, triméthoprime/sulfamethoxazole; AMC, amoxicillin/clavulanate (2/1); GEN, gentamicin; ERY, erythromycin.</p

    Effects of PAĂźN on the antibiotic susceptibility of <i>B. thailandensis</i> strains.

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    <p>Abbreviations: PAĂźN, phenylalanine-arginine Ăź-naphthylamide; DOX, doxycycline; SXT, trimethoprim/sulfamethoxazole; NAL, nalidixic acid; CHL, chloramphenicol; TGC, tigecycline; CAZ, ceftazidime; CTX, cefotaxime; CIP, ciprofloxacin; GEN, gentamicin; NOR, norfloxacin; AMK, amikacin; AMC, amoxicillin/clavulanate (2/1); IPM, imipenem; ATM, aztreonam.</p

    Analyses of the detergent-insoluble membrane proteins of doxycycline-resistant variants.

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    <p>SDS-PAGE analysis was performed on the E264 wild-type strain of <i>B. thailandensis</i>, and on the four doxycycline-resistant derivative strains, E264DC16, E264DC32, E264DC64 and E264DC128. Proteins were stained with Coomassie blue. The variants presented additional bands at around 58 kDa (band A), 55 kDa (band B) and at 54 kDa (bands C and band D). Molecular weight standards are indicated in kilodaltons.</p

    Susceptibility of <i>B. thailandensis</i> strains to cyclines, and antibiotics from other classes.

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    <p>Antimicrobial agent abbreviations: DOX, doxycycline; TET, tetracycline; MIN, minocycline; TGC, tigecycline; CHL, chloramphenicol; NAL, nalidixic acid; CIP, ciprofloxacin; LVX, levofloxacin; SXT, trimethoprim/sulfamethoxazole; ERY, erythromycin; CST, colistin.</p
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