4 research outputs found

    TEAD1 mediates YAP-dependent S100A7 expression.

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    <p>(a) Western blot analyses for S100A7 expression after TEAD1 silencing in normal attached HCC94 cells. (b) The mRNA expression of <i>TEAD1</i>, <i>CTGF</i>, <i>CYR61</i> and <i>S100A7</i> is examined using qRT-PCR. H: HCC94 cells. Error bar, SD of three different experiments. <i>*P<0</i>.<i>05</i>, **<i>P</i><0.01; <i>t-test</i>. (c, d) Overexpression of YAP-WT and mutant activated YAP-S94A in normal attached HCC94 (c) and FaDu (d) cells. Anti-Flag tag antibody was used to judge the transfection efficiency. β-actin was used as a loading control.</p

    The precautionary principle and genetically modified organisms: a bone of contention between European institutions and member states

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    Theoretical thesis.Bibliography: pages 75-86.Introduction -- Chapter I. The precautionary principle -- Chapter II. The precautionary principle and genetically modified organisms under the European legal framework -- Chapter III. The precautionary principle and genetically modified organisms : analysis of three seminal cases of the European Court of Justice (2003-2011) -- Chapter IV. A high level of protection for health and the environment vs free circluation of GMOs on the European market : interests in conflict -- Bibliography.This dissertation examines how the Precautionary Principle, as an internationally recognised concept enshrined in a range of legal instruments, has been applied to provide a mechanism for protection of the environment and health in response to the introduction of Genetically Modified Organisms (GMOs) in Europe. It examines how the European Court of Justice substantively handled the risk assessment phase across three seminal cases between 2003 and 2011 in which Member States had failed in their attempt to trigger the Precautionary Principle in order to uphold a ban or suspension of the cultivation or sale of products derived from GMOs in their territory. The analysis of these judgements suggests that the Court has applied a narrow approach to the evidence provided by national governments during the risk assessment stage, and has thereby limited the potential for precautionary measures by Member States to be upheld by the Court. This outcome reflects a ‘weak’ application of the Precautionary Principle by the Court in contrast with the ‘strong’ interpretation implied by the European legal and policy framework and objections of Member States.Mode of access: World wide web1 online resource (86 pages

    The Hippo pathway is responsible for S100A7 induction in well differentiated cervical and pharyngeal SCC cells.

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    <p>(a, b) Overexpression of LATS1 in normal attached HCC94 (a, left), FaDu (a, right), SiHa (b, left) and H226 (b, right) cells. Anti-Flag tag antibody was used to judge the transfection efficiency. HCC94-WT was used as the positive control. β-actin was used as a loading control (c, d) Depletion of LATS1 using siRNA attenuates S100A7 in suspended or dense HCC94 (c) and FaDu (d) cells. siLATS1+S48h (or D48h) indicates that cells are cultured in suspension (or dense) for 48 h after silencing of LATS1. (e, f) Silencing MST1 in HCC94 (e) and FaDu (f) cells cultured in suspension or dense. siMST1+S48h (or D48h) indicates that cells are cultured in suspension (or dense) for 48 h after losing of MST1. GAPDH was used as a loading control.</p
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