8 research outputs found

    International journal of antimicrobial agents

    No full text
    <p><b>VWF propeptide concentrations are elevated in melioidosis (A) and correlate with VWF antigen levels (B), but do not correlate with mortality (C).</b> VWF = von Willebrand factor. The data from 34 melioidosis patients (of whom 12 died) and 52 controls are presented as box plots with Tukey whiskers showing the smallest observation, lower quartile, median, upper quartile and largest observation. ***<i>P</i> <0.001 for the difference between patients and controls; (Student’s t-test); <i>P</i> = 0.21 for the difference between survivors (n = 22) and non-survivors (n = 12). For the scatter plot, each dot represents a single study subject from the patient group only (n = 34); the correlation coefficient and <i>*P</i> <0.05 reported are for Pearson’s <i>r</i>. The corresponding regression line for the scatter plot is drawn in bold, with the 95% confidence interval for the regression line marked by interrupted lines.</p

    Profound changes in fecal microbiota composition during melioidosis.

    No full text
    <p>Fecal pellets were sampled from eight mice before (t = 0) they were infected intranasally with 150 CFU of <i>B</i>. <i>pseudomallei</i> and 72 hours after (t = 72). Microbial composition was analysed by IS-pro, using the number of nucleotides between the genes for ribosomal subunit 16 and 23 in the DNA (interspacer region) of the bacterium as a unique classification characteristic. (A) Clustering analysis, by unweighted pair group method with arithmetic mean (UPGMA) on cosine distances, shows the similarity of samples; individual mice are indicated by a number. Colors represent the most important bacterial phyla (purple, Actinobacteria; red, Bacteroidetes; blue, Firmicutes, Actinobacteria, Fusobacteria, and Verrucomicrobia (FAFV); yellow, Proteobacteria). Length of the interspacer regions in basepairs is indicated on the y-axis; lines indicate the presence of PCR products. Color intensity increases with the presence of PCR product. (B) Diversity of microbial communities before and 72 hours after induction of melioidosis, expressed as Shannon index (green: total bacteria; red: Bacteroidetes; Blue: Firmicutes, Actinobacteria, Fusobacteria, and Verrucomicrobia (FAFV); yellow: Proteobacteria). Data are presented as box- and whisker plots showing the smallest observation, lower quartile, median, upper quartile and largest observation. ** p<0.01 pre- versus post-infection.</p

    Antibiotic microbiota disruption does not affect neutrophil influx.

    No full text
    <p>Lungs were obtained at the indicated time points after intranasal inoculation with 500 CFU <i>B</i>. <i>pseudomallei</i>. Paraffin-embedded lung tissue sections were stained with haematoxylin/eosin to score different parameters for pathology (A-B, 2x magnification, representative images). The combined score, given by a blinded pathologist, was not different between the two groups (C). Sections from the same samples were stained for Ly-6GC as a neutrophil marker (representative microphotographs, 2x magnification) (D-E). The percentage Ly-6GC-positive surface of the total lung surface was calculated using ImageJ (F). The number of cells per mL BALF was counted using a Coulter counter (G). Myeloperoxidase was quantified in lung homogenates as a measure for neutrophil degranulation (H). Bone marrow and blood was obtained from naĂŻve control and antibiotic pre-treated mice and using FACS analysis the percentage of Ly6GC+, CD11b+ cells within the viable CD45+ population was determined (I). Data are presented as box- and whisker plots showing the smallest observation, lower quartile, median, upper quartile and largest observation. White bars represent control mice, grey bars antibiotic treated mice. No statistically significant differences were found.</p

    Limited effect of antibiotic induced gut microbiota disruption on survival and organ damage.

    No full text
    <p>Survival (A) and clinical observation score (B) of control (white dots) and antibiotic treated mice (grey dots) after intranasal inoculation with 150 CFU <i>B</i>. <i>pseudomallei</i> (n = 20 mice per group, depicted is the mean). No statistically significant differences were detected. Aspartate aminotranspherase (AST, C), alanine aminotranspherase (ALT, D), urea (E) and lactate dehydrogenase (LDH, F) were measured in plasma after inoculation with 500 CFU <i>B</i>. <i>pseudomallei</i> as markers for liver-, renal- and general damage. Data are presented as box- and whisker plots showing the smallest observation, lower quartile, median, upper quartile and largest observation. White bars represent control mice, grey bars antibiotic treated mice. N = 5–6 samples per group. ND = not detectable.</p

    Antibiotic pre-treated mice show increased growth and dissemination of <i>B</i>. <i>pseudomallei</i> during experimental melioidosis.

    No full text
    <p>(A) Study design. (B) Before infection, the fecal microbiota of control- and antibiotic treated mice was analysed by IS-pro. Colors represent the most important bacterial phyla (purple, Actinobacteria; red, Bacteroidetes; blue, Firmicutes, Actinobacteria, Fusobacteria, and Verrucomicrobia (FAFV); yellow, Proteobacteria). Length of the interspacer regions in basepairs is indicated on the x-axis; height of the peaks indicates the presence of PCR products. Samples are pooled from eight mice per group; representative of two experiments. Control and antibiotic pre-treated mice were inoculated intranasally with 150 CFU (C-E) or 500 CFU (F-H) <i>B</i>. <i>pseudomallei</i> and sacrificed at the indicated time points. Bacterial loads in lung homogenate (C, F), blood (D, G) and liver homogenate (E, H) are depicted as scatter dot plots with a line at the median. Numbers in the boxes below (D) and (G) indicate the number of positive blood cultures for the total number of mice. White dots represent control mice, grey dots antibiotic treated mice. N = 6–8 mice per group. * p<0.05, ** p<0.01, *** p<0.001 control versus antibiotic treated.</p
    corecore