Sensitivity of BxPC3 cells to glc-IONPs and PVP-IONPs.

<p>Cells were treated for 1, 3, 6 and 24h with increasing concentrations. IONPs and cell viability was determined by MTT assay. Values are the mean (±SD) of three independent experiments.</p

High Resolution TEM micrograph, magnification 600000x (A) and FFT image performed on a particle of glc-IONP (B).

<p>High Resolution TEM micrograph, magnification 600000x (A) and FFT image performed on a particle of glc-IONP (B).</p

Magnetization (emu·g^-1) vs. applied field (Oe) at 4 K.

<p>Magnetization (emu·g^-1) vs. applied field (Oe) at 4 K.</p

Preparation of Glucose-coated iron nanoparticles using our metal vapor synthesis protocol.

<p>Preparation of Glucose-coated iron nanoparticles using our metal vapor synthesis protocol.</p

GLUT1 levels estimated by Western blotting in BxPC3 and MRC5 cells.

<p>The immunoblot (left panel) bands were analyzed (right panel) for the intensity of the areas by OD with Image J software.</p

TEM micrographs: a) glc-IONP (magnification 250000x); b) PVP-IONP (magnification 160000x).

<p>TEM micrographs: a) glc-IONP (magnification 250000x); b) PVP-IONP (magnification 160000x).</p

TEM images at 20 nm zoom of: a) glc-IONP-treated cells; b) PVP-IONP-treated cells.

<p>TEM images at 20 nm zoom of: a) glc-IONP-treated cells; b) PVP-IONP-treated cells.</p

BxPC3 cells were incubated with 10 and 50 mcg/mL of glc-IONP (panel A) and PVP-IONP (panel B) for 1, 3 or 6h.

<p>The Fe cellular content was estimated by means of GF-AAS analysis. Panel C represents the percentage of inhibition of internalization of PVP-IONP and glc-IONP after pre-tratment with anti GLU1 antibody. Values are the mean (±SD) of three independent experiments.</p