4 research outputs found

    Production of a new anti-A monoclonal reagent

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    Monoclonal antibodies are essential tools in molecular and cellular immunology research. They have essentially replaced the polyclonal antibodies in identifying blood groups and detecting cell markers and pathogenic agents. The aim of the present study is to produce monoclonal antibody identifying the ABO blood groups using the murine hybridoma technology. An anti-A monoclonal antibody A907 was selected and estimated for its use in the manufacture of a reagent anti-A. The selected antibody specifically reacts with A1, A2/sub>, A1B and A2B erythrocytes. It does not recognize B, O, A3 and Ax erythrocytes. The A907 monoclonal antibody can be used in blood grouping in association with a reagent recognizing the A weak phenotypes.Key words: Anti-A monoclonal antibodies, murine myeloma cells, haemagglutination.African Journal of Biotechnology Vol. 4 (8), pp. 844-84

    Fenugreek seeds estrogenic activity in ovariectomized female rats

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    The estrogenic activities of fenugreek seeds (Trigonella foenum-graecum L.), widely used in traditional pharmacopoeia, are reflected in the uterus of ovariectomized female rats, with a slight increase in dry and wet weight, a thickening of the stroma and the uterine epithelium and the development of the endometrial glands. In the vagina, the estrogenic action is shown through an increase in the epidermal cell number and a tendency to keratinization, leading to vaginal opening

    The Effectiveness of a Learner-Centered Pedagogical Approach with Flipped Pedagogy and Digital Learning Environment in Higher Education Feedback on a Cell Biology Course

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    This paper aims to examine the results of a survey of 292 first-year biology students to evaluate their perception of their learning environment, as well as their ability to handle an educational innovation such as reverse pedagogy and serious gaming integrated into a hybrid learning environment. We also present the approaches and steps that allowed us to validate our statistical study. We used a methodology to validate the instrument developed; using standard parameters, the mean and the standard deviation to describe quantitative data and percentages for qualitative data. The internal consistency of the scales was assessed using the correlation coefficient calculated after a one-factor analysis of variance as well as Cronbach's alpha coefficient (α). The scores show that the items and subscales have good internal consistency. In addition, it shows that the students are fully aware of their learning environment and the difficulties they encounter. They are motivated to embrace our pedagogical innovation. It, therefore, confirms our willingness to implement this pedagogical model for students in the coming years

    Pancreatic surgery outcomes: multicentre prospective snapshot study in 67 countries

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    Background: Pancreatic surgery remains associated with high morbidity rates. Although postoperative mortality appears to have improved with specialization, the outcomes reported in the literature reflect the activity of highly specialized centres. The aim of this study was to evaluate the outcomes following pancreatic surgery worldwide.Methods: This was an international, prospective, multicentre, cross-sectional snapshot study of consecutive patients undergoing pancreatic operations worldwide in a 3-month interval in 2021. The primary outcome was postoperative mortality within 90 days of surgery. Multivariable logistic regression was used to explore relationships with Human Development Index (HDI) and other parameters.Results: A total of 4223 patients from 67 countries were analysed. A complication of any severity was detected in 68.7 percent of patients (2901 of 4223). Major complication rates (Clavien-Dindo grade at least IIIa) were 24, 18, and 27 percent, and mortality rates were 10, 5, and 5 per cent in low-to-middle-, high-, and very high-HDI countries respectively. The 90-day postoperative mortality rate was 5.4 per cent (229 of 4223) overall, but was significantly higher in the low-to-middle-HDI group (adjusted OR 2.88, 95 per cent c.i. 1.80 to 4.48). The overall failure-to-rescue rate was 21 percent; however, it was 41 per cent in low-to-middle-compared with 19 per cent in very high-HDI countries.Conclusion: Excess mortality in low-to-middle-HDI countries could be attributable to failure to rescue of patients from severe complications. The authors call for a collaborative response from international and regional associations of pancreatic surgeons to address management related to death from postoperative complications to tackle the global disparities in the outcomes of pancreatic surgery (NCT04652271; ISRCTN95140761)
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