4 research outputs found

    Y├╝n keratozu bazl─▒ hidrojeller ve kendili─činden d├╝zenlenebilen nanoyap─▒lar

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    Thesis (Master)--Izmir Institute of Technology, Chemical Engineering, Izmir, 2017Includes bibliographical references (leaves: 45-52)Text in English; Abstract: Turkish and EnglishIn this study, water soluble keratose proteins were extracted from ÔÇťOvis ariesÔÇŁ wool using peracetic acid oxidation with a yield of 35 ┬▒ 5 %. Wool samples and the extracted keratose proteins were characterized by using FT-IR, XRD, SEM and TGA techniques. ╬▒-keratose fractions (MW = 43-53 kDa) along with cleaved fragments of ╬▒-keratoses with molecular weights between 23 and 33 kDa were identified in the extracted protein mixture using SDS-PAGE analysis. DLS and AFM experiments indicated self-assembled globular nanoparticles with diameters of 20-40 nm formed at 5 and 10 mg/ml keratose concentrations. On the other hand, at 10 % w/v keratose concentration interconnected keratose hydrogels with pore sizes of 6 ┬▒ 4 and 7 ┬▒ 4 ╬╝m were obtained upon incubation at 37 and 50 ┬░C, respectively. Storage moduli (GÔÇÖ) of these physical hydrogels were increased from ~100 to ~1000 Pa, as gelation temperature was increased from 37 to 50 ┬░C. Hydrogels were also obtained at 7.5 % w/v keratose concentration by the addition of a crosslinker, THPC. Amine group:crosslinker ratio was used as 1:1, 1:2 and 1:4. As the amount of crosslinker increased, network transformed from fibrous to more planar structures exhibiting a significant decrease in average pore size from 24 to 11 ╬╝m. GÔÇÖ values of the crosslinked hydrogels were obtained between ~1 and ~5 kPa tuned by the crosslinking amount. Cell interaction properties of a select physical hydrogel prepared at 37 ┬░C was tested using CCK-8 assay. It was observed that the keratose hydrogel supported L929 mouse fibroblast cell proliferation as much as collagen, which suggests that these keratose hydrogels can be promising candidates in soft tissue engineering applications.Bu ├žal─▒┼čmada, keratoz proteinleri, ÔÇťOvis ariesÔÇŁ ad─▒ verilen evcil koyun t├╝r├╝n├╝n y├╝n├╝nden, perasetik asit oksidasyonu kullan─▒larak yakla┼č─▒k 35% verim ile ├ž─▒kart─▒lm─▒┼čt─▒r. Y├╝nler ve elde edilen keratoz proteinleri, FT-IR, XRD, SEM ve TGA teknikleri ile karakterize edilmi┼čtir. SDS-PAGE analizi ile bu protein kar─▒┼č─▒m─▒n─▒n 43-53 kDa molek├╝l a─č─▒rl─▒─č─▒na sahip olan ╬▒-keratoz proteinlerinden ve 23-33 kDa aral─▒─č─▒nda bu proteinlerin par├žalar─▒ndan olu┼čtu─ču saptanm─▒┼čt─▒r. DLS ve AFM testleri, 5 ve 10 mg/ml deri┼čime sahip keratoz ├ž├Âzeltilerinin 20-40 nm hidrodinamik ├žapa sahip k├╝resel nanotaneciklere kendili─činden d├╝zenlendi─čini g├Âstermi┼čtir. ├ľte yandan, keratoz konsantrasyonu 100 mg/ml seviyesine ├ž─▒kar─▒ld─▒─č─▒nda ve s─▒ras─▒yla 37 ve 50 ┬░C s─▒cakl─▒klar alt─▒nda ink├╝be edildi─činde, ortalama g├Âzenek boyutlar─▒ s─▒ras─▒yla 6 ┬▒ 4 and 7 ┬▒ 4 ╬╝m olan hidrojel a─člar─▒n─▒n elde edildi─či g├Âr├╝lm├╝┼čt├╝r. Bu fiziksel hidrojellerin ink├╝basyon s─▒cakl─▒klar─▒n─▒n 37 ┬░CÔÇÖden 50 ┬░C ye ├ž─▒kar─▒lmas─▒yla, saklanan mod├╝l (GÔÇÖ) de─čerinin 100 PaÔÇÖdan 1000 Pa de─čerine ├ž─▒kt─▒─č─▒ g├Âzlemlenmi┼čtir. Hidrojeller ayr─▒ca k├╝tlece % 7.5 keratoz konsantrasyonuna sahip olan protein ├ž├Âzeltilerine THPC kimyasal ├žapraz ba─člama ajanlar─▒ eklenerek de olu┼čturulmu┼čtur. 1:1, 1:2 ve 1:4 amin grubu:├žapraz ba─člay─▒c─▒ oranlar─▒nda 3 farkl─▒ tipte kimyasal hidrojel haz─▒rlanm─▒┼čt─▒r. Bu oran artt─▒k├ža, jel a─č─▒n─▒n i─čsi yap─▒dan d├╝zlemsel yap─▒ya do─čru de─či┼čti─či ve ortalama g├Âzenek ├žap─▒n─▒n 24 ╬╝mÔÇÖden 11 ╬╝mÔÇÖye d├╝┼čt├╝─č├╝ g├Âzlemlenmi┼čtir. 1 kPa ve 5 kPa aras─▒nda elde edilen GÔÇÖ de─čerlerinin THPC miktar─▒n─▒n de─či┼čimi ile kontrol edilebildi─či saptanm─▒┼čt─▒r. 37 ┬░CÔÇÖde haz─▒rlanm─▒┼č se├žilmi┼č fiziksel hidrojelin h├╝cre etkile┼čim ├Âzellikleri CCK-8 y├Ântemi ile test edilmi┼čtir. Bu hidrojelin L929 fare fibroblast h├╝crelerinin b├╝y├╝mesini kollajen kadar destekledi─či g├Âr├╝lm├╝┼č ve keratoz bazl─▒ hidrojellerin yumu┼čak doku m├╝hendisli─činde gelecek vaat eden adaylar olabilece─či ├Âng├Âr├╝lm├╝┼čt├╝r

    Y├╝n keratozu bazl─▒ hidrojeller ve kendili─činden d├╝zenlenebilen nanoyap─▒lar

    No full text
    Thesis (Master)--Izmir Institute of Technology, Chemical Engineering, Izmir, 2017Includes bibliographical references (leaves: 45-52)Text in English; Abstract: Turkish and EnglishIn this study, water soluble keratose proteins were extracted from ÔÇťOvis ariesÔÇŁ wool using peracetic acid oxidation with a yield of 35 ┬▒ 5 %. Wool samples and the extracted keratose proteins were characterized by using FT-IR, XRD, SEM and TGA techniques. ╬▒-keratose fractions (MW = 43-53 kDa) along with cleaved fragments of ╬▒-keratoses with molecular weights between 23 and 33 kDa were identified in the extracted protein mixture using SDS-PAGE analysis. DLS and AFM experiments indicated self-assembled globular nanoparticles with diameters of 20-40 nm formed at 5 and 10 mg/ml keratose concentrations. On the other hand, at 10 % w/v keratose concentration interconnected keratose hydrogels with pore sizes of 6 ┬▒ 4 and 7 ┬▒ 4 ╬╝m were obtained upon incubation at 37 and 50 ┬░C, respectively. Storage moduli (GÔÇÖ) of these physical hydrogels were increased from ~100 to ~1000 Pa, as gelation temperature was increased from 37 to 50 ┬░C. Hydrogels were also obtained at 7.5 % w/v keratose concentration by the addition of a crosslinker, THPC. Amine group:crosslinker ratio was used as 1:1, 1:2 and 1:4. As the amount of crosslinker increased, network transformed from fibrous to more planar structures exhibiting a significant decrease in average pore size from 24 to 11 ╬╝m. GÔÇÖ values of the crosslinked hydrogels were obtained between ~1 and ~5 kPa tuned by the crosslinking amount. Cell interaction properties of a select physical hydrogel prepared at 37 ┬░C was tested using CCK-8 assay. It was observed that the keratose hydrogel supported L929 mouse fibroblast cell proliferation as much as collagen, which suggests that these keratose hydrogels can be promising candidates in soft tissue engineering applications.Bu ├žal─▒┼čmada, keratoz proteinleri, ÔÇťOvis ariesÔÇŁ ad─▒ verilen evcil koyun t├╝r├╝n├╝n y├╝n├╝nden, perasetik asit oksidasyonu kullan─▒larak yakla┼č─▒k 35% verim ile ├ž─▒kart─▒lm─▒┼čt─▒r. Y├╝nler ve elde edilen keratoz proteinleri, FT-IR, XRD, SEM ve TGA teknikleri ile karakterize edilmi┼čtir. SDS-PAGE analizi ile bu protein kar─▒┼č─▒m─▒n─▒n 43-53 kDa molek├╝l a─č─▒rl─▒─č─▒na sahip olan ╬▒-keratoz proteinlerinden ve 23-33 kDa aral─▒─č─▒nda bu proteinlerin par├žalar─▒ndan olu┼čtu─ču saptanm─▒┼čt─▒r. DLS ve AFM testleri, 5 ve 10 mg/ml deri┼čime sahip keratoz ├ž├Âzeltilerinin 20-40 nm hidrodinamik ├žapa sahip k├╝resel nanotaneciklere kendili─činden d├╝zenlendi─čini g├Âstermi┼čtir. ├ľte yandan, keratoz konsantrasyonu 100 mg/ml seviyesine ├ž─▒kar─▒ld─▒─č─▒nda ve s─▒ras─▒yla 37 ve 50 ┬░C s─▒cakl─▒klar alt─▒nda ink├╝be edildi─činde, ortalama g├Âzenek boyutlar─▒ s─▒ras─▒yla 6 ┬▒ 4 and 7 ┬▒ 4 ╬╝m olan hidrojel a─člar─▒n─▒n elde edildi─či g├Âr├╝lm├╝┼čt├╝r. Bu fiziksel hidrojellerin ink├╝basyon s─▒cakl─▒klar─▒n─▒n 37 ┬░CÔÇÖden 50 ┬░C ye ├ž─▒kar─▒lmas─▒yla, saklanan mod├╝l (GÔÇÖ) de─čerinin 100 PaÔÇÖdan 1000 Pa de─čerine ├ž─▒kt─▒─č─▒ g├Âzlemlenmi┼čtir. Hidrojeller ayr─▒ca k├╝tlece % 7.5 keratoz konsantrasyonuna sahip olan protein ├ž├Âzeltilerine THPC kimyasal ├žapraz ba─člama ajanlar─▒ eklenerek de olu┼čturulmu┼čtur. 1:1, 1:2 ve 1:4 amin grubu:├žapraz ba─člay─▒c─▒ oranlar─▒nda 3 farkl─▒ tipte kimyasal hidrojel haz─▒rlanm─▒┼čt─▒r. Bu oran artt─▒k├ža, jel a─č─▒n─▒n i─čsi yap─▒dan d├╝zlemsel yap─▒ya do─čru de─či┼čti─či ve ortalama g├Âzenek ├žap─▒n─▒n 24 ╬╝mÔÇÖden 11 ╬╝mÔÇÖye d├╝┼čt├╝─č├╝ g├Âzlemlenmi┼čtir. 1 kPa ve 5 kPa aras─▒nda elde edilen GÔÇÖ de─čerlerinin THPC miktar─▒n─▒n de─či┼čimi ile kontrol edilebildi─či saptanm─▒┼čt─▒r. 37 ┬░CÔÇÖde haz─▒rlanm─▒┼č se├žilmi┼č fiziksel hidrojelin h├╝cre etkile┼čim ├Âzellikleri CCK-8 y├Ântemi ile test edilmi┼čtir. Bu hidrojelin L929 fare fibroblast h├╝crelerinin b├╝y├╝mesini kollajen kadar destekledi─či g├Âr├╝lm├╝┼č ve keratoz bazl─▒ hidrojellerin yumu┼čak doku m├╝hendisli─činde gelecek vaat eden adaylar olabilece─či ├Âng├Âr├╝lm├╝┼čt├╝r

    Investigation of Membrane Based Processes for Biomedical Applications

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    As substantial developments were achieved in nanotechnology and polymer engineering, especially in the last few decades, the use of membranes and membrane-based procedures was found to be expanding into more and more research and development areas; including biological engineering, life sciences and biomedical engineering. Not only have they been the main focus of meaningful research, but they have also been the main pieces of the solutions to very thorny problems encountered within a wide range of applications from microfluidics to water treatment, thanks to their versatility, cost-effectiveness and biocompatibility, when compared to conventional separation techniques. To celebrate and embrace these qualities, the current research focuses on several impactful membrane-based approaches including reverse electrodialysis (RED) (Chapters 2 and 3), electrodeionization (EDI) (Chapter 4) and hemodialysis (HD) (Chapter 5) and their implementations for biomedical engineering applications. Moreover, this dissertation aims to offer a helpful reference for novel technologies including the development of an external hollow fiber membrane salt cartridge to boost the performance of a reverse electrodialysis cell, and a unique activated ion exchange technology that could be adapted into selective electrolyte removal in various ion-exchange based aqueous treatment applications. As we continue to thrive to solve the mysteries created by fluid-solid surface interactions, membrane technologies will remain to be a strong tool to navigate the humanity in his endless journey

    Development of an Integrated Salt Cartridge-Reverse Electrodialysis (Red) Device to Increase Electrolyte Concentrations to Biomedical Devices

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    Emerging technologies in nanotechnology and biomedical engineering have led to an increase in the use of implantable biomedical devices. These devices are currently battery powered which often means they must be surgically replaced during a patient’s lifetime. Therefore, there is an important need for a power source that could provide continuous, stable power over a prolonged time. Reverse electrodialysis (RED) based biopower cells have been previously used to generate continuous power from physiologically relevant fluids; however, the low salinity gradient that exists within the body limited the performance of the biopower cell. In this study, a miniaturized RED biopower cell design coupled with a salt cartridge was evaluated for boosting the salt concentration gradient supplied to RED in situ. For the salt cartridge, polysulfone (PSf) hollow fibers were prepared in-house and saturated with NaCl solutions to deliver salt and thereby enhance the concentration gradient. The effect of operational parameters including solution flow rate and cartridge salt concentration on salt transport performance was evaluated. The results demonstrated that the use of the salt cartridge was able to increase the salt concentration of the RED inlet stream by 74% which in turn generated a 3-fold increase in the open circuit voltage (OCV) of the biopower cell. This innovative adaptation of the membrane-based approach into portable power generation could help open new pathways in various biomedical applications
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