24 research outputs found

    Array analysis of genes regulated by the PI3K/AKT pathway in lung epithelial cells.

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    <p>(A) Heatmap representation of expression values of DEGs comparing BEAS-AKT1-E17K, BEAS-PIK3CA-E545K and BEAS-shPTEN to BEAS-C control cells. Hierarchical clustering analysis was perfomed with Euclidean metric distance and WardÔÇÖs linkage rule. (B) The Venn diagram shows the number of common, specific and exclusive DEGs.</p

    Comparison analysis of Diseases and Bio Functions enriched in BEAS-2B cells expressing constitutively active PIK3CA, AKT1 or interfered for PTEN.

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    <p>IPA heatmap displays the top three Disease and Biofunctions enriched by DEGs that are common among BEAS-AKT1-E17K, BEAS-PIK3CA-E545K and BEAS-shPTEN cells (commons, rightmost column) or that are exclusive for each cell line (AKT1, PIK3CA, shPTEN). Diseases and Bio Functions were sorted for p-value, from the most significant (violet) to the least significant (white) values.</p

    Analysis of DEG expression in BEAS-C and derivatives cells treated with pharmacological inhibitors of the PI3K/AKT pathway.

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    <p>BEAS-C cells and derivatives were treated for 24 hours with the indicated doses of MK2206 and LY294002, respectively. <b>(A)</b> The graphs show the mRNA levels (mean ┬▒ SD) of the indicated up-regulated genes in BEAS-2B cells and derivatives. <b>(B)</b> The graphs show the mRNA levels (mean ┬▒ SD) of the indicated down-regulated genes in BEAS-2B cells and derivatives. Bars are mean ┬▒ SD of 3 independent experiments conducted in triplicate, statistical analysis that was performed by Two-way ANOVA and Dunnett's test, p-values as indicated <b>(C)</b> Immunoblot analysis of Phospho-AKT (Ser 473), AKT1 and actin in BEAS-C cells and derivatives.</p

    Correlation between the activation status of the PI3K/AKT pathway and DEG expression in NSCLC cell lines.

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    <p><b>(A)</b> Immunoblot analysis of phosphorylated AKT in NSCLC cell lines. AKT1 and actin were used to normalize AKT phosphorylation and protein loading, respectively. <b>(B)</b> The graph shows mRNA levels of up-regulated DEGs (GDF15, PTGES, S100P) in NSCLC cell lines. Statistical analysis was performed on 3 independent experiments conducted in triplicate and was performed by One-way ANOVA test for each gene relative to each cell line, p-values as indicated <b>(C)</b> The graph shows mRNA levels of three representative down-regulated DEGs (SGK1, IGFBP3, PEG10) in NSCLC cell lines. Bars are mean ┬▒ SD of a representative experiment. Statistical analysis was performed on 3 independent experiments conducted in triplicate and was performed by One-way ANOVA test for each cell line, p-values as indicated.</p

    Validation of common DEGs in BEAS-C and derivatives cells treated with pharmacological inhibitors of the PI3K/AKT pathway.

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    <p><b>A)</b> Analysis of 7 common DEGs enriched in BioFunctions in BEAS-C and derivatives. Bars are mean┬▒SD of 3 independent experiments performed in triplicate. Statistical analysis was performed by One-way ANOVA test and Dunnett's test for each gene. p-values were indicated in the figure. <b>B)</b> BEAS-C cells and derivatives were treated for 24 hours with the indicated doses of LY294002. Graphs show mRNA levels of the indicated genes in BEAS-C cells and derivatives. Bars are mean┬▒SD of 3 independent experiments conducted in triplicate. Statistical analysis was performed by <i>t-</i>test. p-values as indicated in the figure.</p
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