sj-pdf-1-jva-10.1177_11297298241230110 – Supplemental material for Knowledge, attitude, and practice toward arteriovenous fistulas for hemodialysis among nurses

Supplemental material, sj-pdf-1-jva-10.1177_11297298241230110 for Knowledge, attitude, and practice toward arteriovenous fistulas for hemodialysis among nurses by Sifeng Huang and Dongmei Liu in The Journal of Vascular Access</p

sj-pdf-2-jva-10.1177_11297298241230110 – Supplemental material for Knowledge, attitude, and practice toward arteriovenous fistulas for hemodialysis among nurses

Supplemental material, sj-pdf-2-jva-10.1177_11297298241230110 for Knowledge, attitude, and practice toward arteriovenous fistulas for hemodialysis among nurses by Sifeng Huang and Dongmei Liu in The Journal of Vascular Access</p

Additional file 1 of Astragaloside IV restrains pyroptosis and fibrotic development of pulmonary artery smooth muscle cells to ameliorate pulmonary artery hypertension through the PHD2/HIF1α signaling pathway

Supplementary Material

Effects of Cd on ovarian index, hepatopancreatic index, triglyceride and lipid content.

<p>Ovarian index (a), hepatopancreatic index (b), triglyceride levels in hemolymph (e) and lipid content expressed as percentage (weight of lipid/ tissue samples, FW: fresh weight) in the ovary (c) and hepatopancreas (d) for control and exposed crabs after 10, 15, and 20 days of experimentation. Data are expressed as mean ± standard deviation (n = 5). Comparison between the control and treatment groups is notified as * <i>p</i> < 0.05.</p

Pathological changes of the subcellular structure of the <i>S</i><i>. henanense</i> hepatopancreas exposed to Cd.

<p>(<b>A</b>) Normal hepatopancreatic cell nucleus. Bar = 5 µm. (<b>B</b>) Normal mitochondria with a matrix of moderate electron density. Bar = 500 nm. (<b>C</b>) Mitochondria were damaged in the 7.12-mg/L Cd group. Mitochondria showed a matrix with low electron density and discontinuous membrane (➨). Primary lysosomes were seen (⇧). Bar = 500 nm. (<b>D</b>) Mitochondria were damaged in the 14.25-mg/L Cd group. The majority of mitochondria maintained their structure, but with a swollen matrix, reduction of the matrix density, shortening of the cristae, and rupture of a subset of the mitochondrial membrane (➨). Cristae disappeared and double-membrane vesicles were noted (✰) in severely damaged mitochondria. Bar = 500 nm. (<b>E</b>) Apoptotic cell in the 14.25-mg/L group. Chromatin was aggregated under the karyolemma and apoptotic bodies formed. Several morphologically normal mitochondria were seen. Almost all mitochondria turned into vesicles, and other organelles also disappeared. Bar = 2 µm. (<b>F</b>) Shows the autolysosomes and mitochondrion in two cells of the 14.25-mg/L Cd group. Bar = 2 µm. (<b>G</b>) Nucleus of a necrotic cell in the 28.49-mg/L group. The nuclear membrane was ruptured (<b>←</b>) and most organelles were disappeared. Bar = 500 nm. (<b>H</b>) Damaged RER and Mit in the 14.25-mg/L group. Bar = 500 nm (Ly: lysosome; Lip: lipid; Mit: mitochondria; N: nucleus; RER: rough endoplasmic reticulum).</p

Effect of Cd on lipid digestion, lipid transport and lipid synthesis of <i>Sinopotamon henanense</i>.

<p>Activity of pancreatic lipase (PL, a), lipoprotein lipase (LPL, b) fatty acid synthase (FAS) in the hepatopancreas (c) and ovary (d) and acyl-CoA carboxylase (ACC) activity in the hepatopancreas (e) and ovary (f) of control and exposed crabs after 10, 15 and 20 days of experimentation. Comparison between the control and treatment groups is notified as * <i>p</i> < 0.05.</p

Effect of Cd on the content of cytochrome <i>c</i> in the <i>S</i><i>. henanense</i> hepatopancreas.

<p>No significance between the Cd-treated and the control groups were observation except the 56.98 mg/L group markedly reduction, compared with control, different letter indicated <i>p</i> < 0.05 (<i>n</i> = 6).</p

Effect of Cd on hepatopancreas caspase 3, 8, and 9 activities in <i>S</i><i>. henanense</i>.

<p>(<b>A</b>) Caspase 3 activity (<i>n</i> = 6). (<b>B</b>) Caspase 9 activity. (<b>C</b>) Caspase 8 activity (The different small letters were used to indicate the significant difference (<i>p</i> < 0.05) between groups).</p

TUNEL assay for detection of the effect of Cd on hepatopancreas apoptosis in <i>S</i><i>. henanense</i> (<i>n</i> = 4).

<p>(<b>A</b>) Control group. (<b>B</b>) 3.56-mg/L group. (<b>C</b>) 7.12-mg/L group. (<b>D</b>) 14.25-mg/L group. (<b>E</b>) 28.49-mg/L group. (<b>F</b>) 56.98-mg/L group. The apoptotic cellular nucleus was stained brown, ×600. The positive cells were dispersedly distributed or clustered.</p

Cadmium concentrations in the tissues of control and exposed crabs.

<p>Data of hepatopancreas (a) and ovary (b) are expressed as mean ± standard deviation (n = 5). Statistical significance is denoted by * <i>p</i> < 0.05 compared to the respective control.</p