Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism

Schmallenberg virus (SBV) is a newly emerged orthobunyavirus (family Bunyaviridae) that has caused severe disease in the offspring of farm animals across Europe. Like all orthobunyaviruses, SBV contains a tripartite negative-sense RNA genome that is encapsidated by the viral nucleocapsid (N) protein in the form of a ribonucleoprotein complex (RNP). We recently reported the three-dimensional structure of SBV N that revealed a novel fold. Here we report the crystal structure of the SBV N protein in complex with a 42-nt-long RNA to 2.16 Å resolution. The complex comprises a tetramer of N that encapsidates the RNA as a cross-shape inside the protein ring structure, with each protomer bound to 11 ribonucleotides. Eight bases are bound in the positively charged cleft between the N- and C-terminal domains of N, and three bases are shielded by the extended N-terminal arm. SBV N appears to sequester RNA using a different mechanism compared with the nucleoproteins of other negative-sense RNA viruses. Furthermore, the structure suggests that RNA binding results in conformational changes of some residues in the RNA-binding cleft and the N- and C-terminal arms. Our results provide new insights into the novel mechanism of RNA encapsidation by orthobunyaviruses

Improving Stochastic Estimator Techniques for Disconnected Diagrams

Disconnected diagrams are expected to be sensitive to the inclusion of dynamical fermions. We present a feasibility study for the observation of such effects on the nucleonic matrix elements of the axial vector current, using SESAM full QCD vacuum configurations with Wilson fermions on $16^3\times 32$ lattices, at $\beta =5.6$. Starting from the standard methods developed by the Kentucky and Tsukuba groups, we investigate the improvement from various refinements thereof.Comment: One author added. Contribution to Lattice 1997, 3 pages LaTex, to appear in Nucl. Phys. B (Proc. Suppl.

The perils of credit booms

We present a dynamic general equilibrium model of production economies with adverse selection in the financial market to study the interaction between funding liquidity and market liquidity and its impact on business cycles. Entrepreneurs can take on short-term collateralized debt and trade long-term assets to finance investment. Funding liquidity can erode market liquidity. High funding liquidity discourages firms from selling their good long-term assets since these good assets have to subsidize lemons when there is information asymmetry. This can cause a liquidity dry-up in the market for long-term assets and even a market breakdown, resulting in a financial crisis. Multiple equilibria can coexist. Credit booms combined with changes in beliefs can cause equilibrium regime shifts, leading to an economic crisis or expansion.Published versio

A quantum solution to Gibbs Paradox with few particles

We present a fully quantum solution to the Gibbs paradox (GP) with an illustration based on a gedanken experiment with two particles trapped in an infinite potential well. The well is divided into two cells by a solid wall, which could be removed for mixing the particles. For the initial thermal state with correct two-particle wavefunction according to their quantum statistics, the exact calculations shows the entropy changes are the same for boson, fermion and non-identical particles. With the observation that the initial unmixed state of identical particles in the conventional presentations actually is not of a thermal equilibrium, our analysis reveals the quantum origin of the paradox, and confirm the E. J. Jaynes' observation that entropy increase in Gibbs mixing is only due to the including more observables measuring the entropy. To further show up the subtle role of the quantum mechanism in the GP, we study the different finite size effect on the entropy change and shows the works performed in the mixing process are different for various types of particle.Comment: 4 pages, 3 figures + Supplementary Materia