Additional file 7 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 7: Fig. S4. Predicted binding pocket in WT TPP domain. The Mg2+ ion binding site of WTTPP domain was expressed

Additional file 2 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 2: Fig. S1. Whole gel Southern blot result. Lane 1, WT (no digestion); lane 2, Mut68 (no digestion); Lane 3, WT (digested by NheI); lane 4, Mut68 (digested by NheI); Lane 5, WT (digested by NheI and XhoI); lane 6, Mut68 (digested by NheI and XhoI)

Additional file 3 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 3: Table S2. Dry cell weight and biomass productivity analysis of WT and Mut68

Additional file 1 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 1: Table S1. Oligonucleotides used in this study

Additional file 6 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 6: Fig. S3. Predicted protein crystal structure of WT (a) and truncated (b) TPS based on the template-based prediction tool RaptorX. Yellow, TPSWT and TPS Tr domain; cyan, TPPWT and TPPTr domain; purple, N-terminalWT and N-terminalTr domain; orange, C-terminalWT; grey, disordered region

Additional file 5 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 5: Fig. S2. Coding DNA sequence of truncated TPS in Mut68. Inserted pNsShble was highlighted in green and stop codon generated by insertion of pNsShble was underlined

Additional file 4 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 4: Table S3. Comparison of FAME composition of WT and Mut68

MOESM1 of Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution

Additional file 1: Figure S1. Effect of the filE gene deletion on LA tolerance of JHM30. JHY30, JHY31 (JHM30 ΔfliE::KanR), JHM80 were grown in the absence or the presence of 8.0 g/L LA. Error bars indicate standard deviations of three independent experiments

MOESM2 of Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution

Additional file 2: Table S1. Primers used in this study