Isolated glandular trichomes recovered from 30 µm mesh (A) and sucrose gradient purification (B).

<p>NT, non-glandular trichome; GST, glandular secreting trichome.</p

Imagines of up-regulated proteins in glandular trichomes compared with corresponding leaves.

<p>The images on the left side of each panel are: A. p510: NAD(P)H-quinone oxidoreductase subunit M (gi|209573110); B. p667: V-type (H+)-ATPase V1, A subunit (gi|224109966); C. p90: cytochrome C oxidase polypeptide vib (gi|168061725); D. p245: artemisinic aldehyde delta-11(13) reductase (gi|197310860); E. p484: plastidic aldolase NPALDP1 (gi|108864048); F. p467: glyceraldehyde-3 phosphate dehydrogenase (gi|115371630); G. p353: phosphoglycerate mutase 1 (gi|114326546); H. p26: phosphopyruvate hydratase (gi|224080171); I. p88: glycine cleavage system H protein (gi|1346119); J. p106: predicted protein (PRK, gi|168006632); K. p242: nucleoside diphosphate kinase B (gi|1346675); L. p414: 4-aminobutyrate aminotransferase (gi|159477247); M. p83: eukaryotic translation initiation factor eIF5A (gi|85376261); N. p328: putative zinc dependent protease (gi|84468324); O. p200: chloroplast FtsH protease (gi|1483215); P. p191: cell division protein FtsH-like protein (072367_3859_2372_2p); Q. p399: cell division protein FtsH-like protein (072367_3859_2372_2p); R: p139: mitochondrial-processing peptidase subunit alpha (gi|266567); S. p457: cysteine protease (gi|239937266); T. p473: peroxiredoxin (Contig38348_2p_5); U. p244: ABC_NikE_OppD_transporters (gi|218196275). The images on the right side of each panel are 2DE gels for corresponding leaves.</p

Trichome distribution on the leaf surface of <i>A. annua</i> during different developmental stages.

<p>Trichome distribution on the leaf surface of <i>A. annua</i> during different developmental stages.</p

The simplied pathway of terpenoid biosynthsis in plants.

<p>Modified from reported data <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041822#pone.0041822-Pamela1" target="_blank">[48]</a> MVA, 3R-Mevalonic acid; MEP, 2-C-Methy-D-erythritol 4-phosphate; DMAPP, dimethylallyl diphosphate; IPP, isopentenyl diphosphate; FPS, farnesyl diphosphate synthase; HMGR, 3-hydroxy-3-methylglutaryl coenzyme A reductase; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; DXR, 1-deoxy-D-xylulose-5-phosphate reductoisomerase; SQC, sesquiterpene cyclase; SQS, squalene synthase.</p

Categorization and distribution of proteins in glandular trichomes.

<p>Categorization and distribution of proteins in glandular trichomes.</p

Venn diagram of identified proteins in GSTs using NCBI or Artemisia EST databases.

<p>Venn diagram of identified proteins in GSTs using NCBI or Artemisia EST databases.</p

2DE patterns of trichome (A) and leaves (B) proteins.

<p>82 non-redundant proteins in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041822#pone.0041822.s002" target="_blank">Table S1</a> were shown on the gel of glandular trichomes. About 70% of them were highly expressed in the glandular trichomes.</p

2DE patterns of proteins from trichomes (A, B, C) and leaves (D, E, F).

<p>900 µg of proteins were loaded in each gel and stained with “Blue silver” staining solution. Proteins of isolated glandular trichomes of leaves (A, B, C) and corresponding leaves (D, E, F) are separated on pH gradients 4–7. Three biological repeats were obtained in the study.</p

Additional file 1: Table S1. of A heuristic model for computational prediction of human branch point sequence

The formulas of 15 scoring measures (Score0-Score14) and corresponding values for P j , Q j  ∈ [0, 1], j = 1, 2, 3. Table S2. The endpoints (5′- and 3′-) of the shortened AGEZ and corresponding branch sites labeled by their positons relative to the 3’ss for each intron in Additional file 2: Dataset S1 when L = 9. Table S3. The endpoints (5′- and 3′-) of the shortened AGEZ and corresponding branch sites labeled by their positons relative to the 3’ss for each intron in Additional file 3: Dataset S2 when L = 9. Table S4. The relative frequencies of nucleotides at each position for BPSs in Additional file 2: Dataset S1 and corresponding information content (IC). Table S5. The relative frequencies of nucleotides at each position for BPSs in Additional file 3: Dataset S2 and corresponding information content (IC). Table S6. The relative frequencies of nucleotides at each position for 252,302 human BPSs predicted by sequencing method. Table S7. The results of 15 scoring measures (Score0-Score14) for BPS prediction on Additional file 2:Dataset S1 and Additional file 3: Dataset S2. Table S8. The relative frequencies of nucleotides at each position for genome-wide predicted BPSs and corresponding information content (IC) (DOCX 44 kb

Additional file 2: Dataset_S1. of A heuristic model for computational prediction of human branch point sequence

(FASTA 146 kb