34 research outputs found

    Sex ratio, female weight and longevity (mean ┬▒ SE) of <i>Nilaparvata lugens</i> developed on rice germplasms.

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    <p>A: Sex ratio (n = 3). B: Female weight (n = 30). C: Female longevity (n = 15). The different letters over the bars in a panel indicate significant differences (Tukey HSD, <i>P</i> = 0.05).</p

    Developmental duration (A, n = 240) and survival (B, n = 3) (mean ┬▒ SE) of <i>Nilaparvata lugens</i> nymphs on rice germplasms.

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    <p>Different letters over the bars in a panel indicate significant differences (Tukey HSD, <i>P</i> = 0.05).</p

    Evaluation standard for rice resistance to planthoppers based on seedling mortality (adapted from Horgan et al. 2015).

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    <p>Evaluation standard for rice resistance to planthoppers based on seedling mortality (adapted from Horgan et al. 2015).</p

    Resistance to <i>Nilaparvata lugens</i> assessed with the standard seed box test at the seedling stage of rice germplasms.

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    <p>Data are expressed as the mean ┬▒ SE from 10 replicates. Different letters over the bars indicate significant differences (Tukey HSD, <i>P</i> = 0.05).</p

    Weight of honeydew (mean ┬▒ SE, n = 30) excreted by female <i>Nilaparvata lugens</i> adults on rice germplasms.

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    <p>Different letters over the bars indicate significant differences (Tukey HSD, <i>P</i> = 0.05).</p

    Population size (no./100 hills, mean ┬▒ SE) of <i>Nilaparvata lugens</i> in field plots (n = 3) of the TN1, IR64, B5, HHZ, BR4831, 9311, HF106, and C602 rice germplasms.

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    <p>Population size (no./100 hills, mean ┬▒ SE) of <i>Nilaparvata lugens</i> in field plots (n = 3) of the TN1, IR64, B5, HHZ, BR4831, 9311, HF106, and C602 rice germplasms.</p

    Population parameters (mean ┬▒ SE) of <i>Nilaparvata lugens</i> fed on the TN1, IR64, B5, HHZ, BR4831, 9311, HF106, and C602 rice germplasms.

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    <p>Population parameters (mean ┬▒ SE) of <i>Nilaparvata lugens</i> fed on the TN1, IR64, B5, HHZ, BR4831, 9311, HF106, and C602 rice germplasms.</p

    Settling preferences of macropterous female <i>Nilaparvata lugens</i> adults for plants of the rice germplasm at 24 h (A), 72 h (B) and 120 h (C) after insect release in the cage tests.

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    <p>Data are expressed as the mean ┬▒ SE from 10 replicates. Different letters over the bars in a panel indicate significant differences (Tukey HSD, <i>P</i> = 0.05).</p

    Expression profiles of 11 candidate reference genes in <i>Frankliniella occidentalis</i>.

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    <p>The expression levels of the candidate reference genes are documented by the <i>C</i><sub><i>t</i></sub> values. The median is represented by the line in the box. The interquartile rang is bordered by the upper and lower edges, which indicate the 75<sup>th</sup> and 25<sup>th</sup> percentiles, respectively.</p

    A single-step reverse transcription loop-mediated isothermal amplification assay for rapid and accurate detection of <i>Pepper vein yellows virus</i>

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    <p>Pepper vein yellows virus (PeVYV) is a member of Polerovirus and infects solanaceous crops worldwide. In this study, a highly efficient and easy-to-use single-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for PeVYV detection. This assay used two outer (F3 and B3), two inner (FIP and BIP) and two loop (LF and LB) primers in a single reaction. The optimized reaction was determined to be at 62°C for 45 min and the whole reaction took less than 1 h. This assay was about 10 times more sensitive than the conventional one-step RT-PCR. This RT-LAMP assay should allow specific detection of PeVYV in plant tissues. We recommend this PeVYV-specific RT-LAMP assay for large scale field studies of PeVYV infection.</p
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