Fund for Shared Insight: Media Analysis

Fund for Shared Insight ("Shared Insight") is a collaborative effort among fundersthat pools financial and other resources to make grants to improve philanthropy. Shared Insight believes philanthropy can have a greater social and environmental impact if foundations and nonprofits listen to the people they seek to help, act on what they hear, and openly share what they learn.Related to feedback loops, Shared Insight's work is focused on increasingthe extent to which foundations listen to others—especially the people they seek to help—and respond to their expressed interests. When Shared Insight talks about "the people they seek to help," they are referring to the individuals who receive programs and services from nonprofit organizations; for example, the students served by charter schools, the recently released prisoners benefiting from job-training services, and the low-income first-time mothers participating in prenatal through birth programs.Over the next three years, Shared Insight would hope to see changes in the amount and kind of discourse in the field related tobeneficiary feedback loops. In the summer of 2015, one year since the launch of the collaborative, ORS Impact repeated a media analysis of relevant blogs, periodicals, and reports. The following memo outlines changes in the amount and kind of discourse in the field around feedback loops compared to the year before Shared Insight launched. We raise a few observations and considerations. More detailed methodological notes follow

Data_Sheet_1_Mapping and Analysis of a Novel Genic Male Sterility Gene in Watermelon (Citrullus lanatus).PDF

Seed production is critical for watermelon production, which mostly involves first-generation hybrid varieties. However, watermelon hybrid seed production currently requires complex procedures, including artificial isolation and pollination. Therefore, the development and use of a male-sterile system to generate watermelon hybrids can simplify the process. The scarcity of male-sterile watermelon germplasm resources necessitates the use of molecular breeding methods. Unfortunately, the genes responsible for male sterility in watermelon have not been cloned. Thus, the genetic basis of the male sterility remains unknown. In this study, two DNA pools derived from male-sterile and normal plants in the F2 population were used for whole-genome resequencing. The Illumina high-throughput sequencing resulted in 62.99 Gbp clean reads, with a Q30 of 80% after filtering. On the basis of the SNP index association algorithm, eight candidate regions (0.32 Mb) related to specific traits were detected on chromosome 6. Expression pattern analyses and watermelon transformation studies generated preliminary evidence that Cla006625 encodes a pollen-specific leucine-rich repeat protein (ClaPEX1) influencing the male sterility of watermelon. The identification and use of genic male sterility genes will promote watermelon male sterility research and lay the foundation for the efficient application of seed production technology.</p

Additional file 2 of The DELLA proteins interact with MYB21 and MYB24 to regulate filament elongation in Arabidopsis

Additional file 2: Figure S1. Detection of Auto-activation for MYB21, MYB21NT, MYB24, and MYB24NT in Y2H Experiments. MYB21, MYB21NT, MYB24, and MYB24NT were individually fused with the LexA DNA binding domain (BD) in pLexA. The full-length of MYB21 and MYB24 with BD domain exhibited auto-activation, while N-terminal of MYB21 (MYB21NT) and MYB24 (MYB24NT) with BD domain lost auto-activation. Auto-activation (represented by blue color) were assessed on 2% Gal/1% raffinose/SD/−Ura/−His/−Trp/−Leu/X-β-Gal medium

Additional file 3 of The DELLA proteins interact with MYB21 and MYB24 to regulate filament elongation in Arabidopsis

Additional file 3: Figure S2. Negative Controls for the Y2H Experiments. No interaction was detected after co-expression of pB42AD-RGA/RGA-L/RGA-R/GAI/RGL1/RGL2/RGL2-L/RGL2-R/RGL3/MYB21/MYB21NT/MYB21CT/MYB24/MYB24NT/MYB24CT with the BD domain in pLexA empty vector. Interactions were assessed on 2% Gal/1% raffinose/SD/−Ura/−His/−Trp/−Leu/X-β-Gal medium

Additional file 1 of The DELLA proteins interact with MYB21 and MYB24 to regulate filament elongation in Arabidopsis

Additional file 1: Table S1. Primers Used for Vector Constructio

Additional file 11: of Identification of cold stress responsive microRNAs in two winter turnip rape (Brassica rapa L.) by high throughput sequencing

Table S8. Targets prediction of all the sequenced miRNAs (XLS 57 kb

Additional file 4 of The DELLA proteins interact with MYB21 and MYB24 to regulate filament elongation in Arabidopsis

Additional file 4: Figure S3. Source data for Figs. 1e-g and 2f-g. (a-c) Red frame in Figures a-c respectively displayed the source data for Figs. 1e-g. (d) Full scan of SDS-PAGE gel shown in Figs. 1e-g. Red frame from left to right displayed the source data for Fig. 1e, g, and f, respectively. Asterisks indicated the positions of purified MBP, MBP-MYB21 and MBP-MYB24. (e) Full scan of the results shown in Figs. 2f-g. Red frame from left to right respectively displayed the source data for Fig. 2f, and g. (f) Red frame from left to right respectively displayed the source data for Figs. 2f, and 2 g

Additional file 3: of Identification of cold stress responsive microRNAs in two winter turnip rape (Brassica rapa L.) by high throughput sequencing

Figure S1. The correlation analysis of miRNA expression between two biological duplicates (JPEG 3923 kb

Additional file 4: of Identification of cold stress responsive microRNAs in two winter turnip rape (Brassica rapa L.) by high throughput sequencing

Table S3. The conserved miRNAs identified in winter turnip rape (XLS 76 kb

唐代渭河流域与泾河流域涝灾研究

Table S7. Differentially expressed miRNAs between two varieties (DOC 41 kb