Additional file 1: of Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditions

Results of PEDV N-gene RT-qPCR tests on environmental samples from coupons and rectal swabs from pigs used for bioassay. Results from all diagnostic tests conducted for the study are reported for each replicate, one row for each replicate. Test results, Ct value and genomic copies are reported for PCR tests on pre and post treatment environmental samples from coupons. Test result and Ct values are reported for PCR tests on rectal swabs from pigs 3 and 7 days post inoculation for the bioassay. (XLSX 12 kb

Overall half-lives.

Representation of the overall half-life in hours for PEDV, PRRSV 1-4-4 L1C variant, and PRRSV MN 184.</p

PRRSV titration results.

PRRSV MN 1-8-4 and PRRSV 1-4-4 L1C Variant titration results per surface, contact time and temperature. (PDF)</p

PEDV titration results.

Results of PEDV titrations per surface, contact time and temperature. (PDF)</p

Half-lives per surface.

Representation of the half-life in hours for each virus (PEDV, PRRSV 1-4-4 L1C variant, and PRRSV MN 184) by each surface type (aluminum and cardboard).</p

Summary of results.

Fomites might be responsible for virus introduction in swine farms, highlighting the importance of implementing practices to minimize the probability of virus introduction. The study’s objective was to assess the efficacy of different combinations of temperatures and holding-times on detecting live PRRSV and PEDV on surfaces commonly found in supply entry rooms in swine farms. Two PRRSV isolates (MN 184 and 1-4-4 L1C variant) and one PEDV isolate (NC 49469/2013) were inoculated on cardboard and aluminum. An experimental study tested combinations of four temperatures (20°C, 30°C, 40°C, and 50°C) and six holding-times (15 minutes, 60 minutes, 6 hours, 12 hours, 24 hours, and 36 hours) for the presence of the viruses on each surface type. After virus titration, virus presence was assessed by assessing the cytopathic effects and immunofluorescence staining. The titers were expressed as log10 TCID50/ml, and regression models; half-lives equations were calculated to assess differences between treatments and time to not detect the live virus. The results suggest that the minimum time that surfaces should be held to not detect the virus at 30°C was 24 hours, 40°C required 12 hours, and 50°C required 6 hours; aluminum surfaces took longer to reach the desired temperature compared to cardboard. The results suggest that PRRSV 1-4-4 L1C variant had higher half-lives at higher temperatures than PRRSV MN 184. In conclusion, time and temperature combinations effectively decrease the concentration of PRRSV and PEDV on different surfaces found in supply entry rooms in swine farms.</div

Study design.

Description of the surfaces, viruses, temperatures and times evaluated in this study.</p