A Method to Solve Non-homogeneous Strongly Coupled Mixed Parabolic Boundary Value Systems with Non-homogeneous Boundary Conditions

In this paper, a method to construct the solution of non-homogeneous parabolic coupled systems with non-homogeneous boundary conditions of the type ut−Auxx = G(x, t), A1u(0, t)+B1ux(0, t) = P(t), A2u(l, t)+ B2ux(l, t) = Q(t), 0 0, u(x, 0) = f(x), where A is a positive stable matrix and A1, A2, B1, B2 are arbitrary matrices for which the block matrix A1 B1 A2 B2 is non-singular, is proposed. Two illustrative examples of the method are given.Soler Basauri, V.; Defez Candel, E.; Capilla Lladró, R. (2015). A Method to Solve Non-homogeneous Strongly Coupled Mixed Parabolic Boundary Value Systems with Non-homogeneous Boundary Conditions. International Journal of Mathematical Analysis. 9(40):1955-1970. doi:10.12988/ijma.2015.57176S1955197094

On the Construction of Analytic-Numerical Approximations for a Class of Coupled Differential Models in Engineering

In this paper, a method to construct an analytic-numerical solution for homogeneous parabolic coupled systems with homogeneous boundary conditions of the type ut = Auxx, A1u(o,t) + B1ux(o,t) = 0, A2u(1,t) + B2ux(1,t) = 0, ot>0, u (x,0) = f(x), where A is a positive stable matrix and A1, B1, B1, B2, are arbitrary matrices for which the block matrix is non-singular, is proposed.Defez Candel, E.; Soler Basauri, V.; Capilla Lladró, R. (2015). On the Construction of Analytic-Numerical Approximations for a Class of Coupled Differential Models in Engineering. Open Journal of Modelling and Simulation. 3:1-18. doi:10.4236/ojmsi.2015.31001S118

On the Exact Series Solution for Nonhomogeneous Strongly Coupled Mixed Parabolic Boundary Value Problems

This paper studies the construction of the exact solution for parabolic coupled systems of the type [u.sub.t] = A[u.sub.xx], [A.sub.1]u(0, t) + [B.sub.1][u.sub.x](0, t) = 0, [A.sub.2] u(l, t) + [B.sub.2][u.sub.x](l, t) = 0, 0 0, and u(x, 0) = f(x), where [A.sub.1], [A.sub.2], [B.sub.1], and [B.sub.2] are arbitrary matrices for which the block matrix...Soler Basauri, V.; Defez Candel, E.; Capilla Lladró, R.; Verdoy González, JA. (2014). On the Exact Series Solution for Nonhomogeneous Strongly Coupled Mixed Parabolic Boundary Value Problems. Abstract and Applied Analysis. 2014:1-10. doi:10.1155/2014/826860S1102014Melezhik, V. ., Puzynin, I. ., Puzynina, T. ., & Somov, L. . (1984). Numerical solution of a system of integrodifferential equations arising from the quantum mechanical three-body problem with coulomb interaction. Journal of Computational Physics, 54(2), 221-236. doi:10.1016/0021-9991(84)90115-3Levine, R. D., Shapiro, M., & Johnson, B. R. (1970). Transition Probabilities in Molecular Collisions: Computational Studies of Rotational Excitation. The Journal of Chemical Physics, 52(4), 1755-1767. doi:10.1063/1.1673214Lill, J. V., Schmalz, T. G., & Light, J. C. (1983). Imbedded matrix Green’s functions in atomic and molecular scattering theory. The Journal of Chemical Physics, 78(7), 4456-4463. doi:10.1063/1.445338Mrugal/a, F., & Secrest, D. (1983). The generalized log‐derivative method for inelastic and reactive collisionsa). The Journal of Chemical Physics, 78(10), 5954-5961. doi:10.1063/1.444610Soler, V., Defez, E., Ferrer, M. V., & Camacho, J. (2013). On Exact Series Solution of Strongly Coupled Mixed Parabolic Problems. Abstract and Applied Analysis, 2013, 1-9. doi:10.1155/2013/524514Soler, V., Defez, E., & Verdoy, J. A. (2014). On Exact Series Solution for Strongly Coupled Mixed Parabolic Boundary Value Problems. Abstract and Applied Analysis, 2014, 1-9. doi:10.1155/2014/759427Navarro, E., Jódar, L., & Ferrer, M. V. (2002). Constructing eigenfunctions of strongly coupled parabolic boundary value systems. Applied Mathematics Letters, 15(4), 429-434. doi:10.1016/s0893-9659(01)00154-

Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109

Bradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It is known for its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. japonicum E109 was recently analyzed and it showed the presence of genes related to the synthesis of IAA by indole-3-acetonitrile, indole-3-acetamide and tryptamine pathways. Nevertheless, B. japonicum E109 is not able to produce IAA and instead has the ability to degrade this hormone under saprophytic culture conditions. This work aimed to study the molecular and physiological features of IAA degradation and identify the genes responsible of this activity. In B. japonicum E109 we identified two sequences coding for a putative 3-phenylpropionate dioxygenase (subunits α and β) responsible for the IAA degradation that were homologous to the canonical cluster of iacC and iacD of Pseudomonas putida 1290. These genes form a separate cluster together with three additional genes with unknown functions. The degradation activity was found to be constitutively expressed in B. japonicum E109. As products of IAA degradation, we identified two compounds, 3-indoleacetic acid 2,3-oxide and 2-(2-hydroperoxy-3-hydroxyindolin-3-yl) acetic acid. Our report proposes, for the first time, a model for IAA degradation in Bradyrhizobium.Fil: Torres, Daniela Soledad. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaFil: Donadío, Evelyn Florencia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Donoso, Raúl. Universidad Tecnológica Metropolitana; ChileFil: Recabarren Gajardo, Gonzalo. Pontificia Universidad Católica de Chile; ChileFil: Gualpa, José Luis. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Spaepen, Stijn. Katholikie Universiteit Leuven; BélgicaFil: Defez, Roberto. Institute Of Biosciences And Bioresources; ItaliaFil: Lopez, Gaston Alberto. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Bianco, Carmen. Institute Of Biosciences And Bioresources; ItaliaFil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentin

Sex difference and intra-operative tidal volume: Insights from the LAS VEGAS study

BACKGROUND: One key element of lung-protective ventilation is the use of a low tidal volume (VT). A sex difference in use of low tidal volume ventilation (LTVV) has been described in critically ill ICU patients.OBJECTIVES: The aim of this study was to determine whether a sex difference in use of LTVV also exists in operating room patients, and if present what factors drive this difference.DESIGN, PATIENTS AND SETTING: This is a posthoc analysis of LAS VEGAS, a 1-week worldwide observational study in adults requiring intra-operative ventilation during general anaesthesia for surgery in 146 hospitals in 29 countries.MAIN OUTCOME MEASURES: Women and men were compared with respect to use of LTVV, defined as VT of 8 ml kg-1 or less predicted bodyweight (PBW). A VT was deemed 'default' if the set VT was a round number. A mediation analysis assessed which factors may explain the sex difference in use of LTVV during intra-operative ventilation.RESULTS: This analysis includes 9864 patients, of whom 5425 (55%) were women. A default VT was often set, both in women and men; mode VT was 500 ml. Median [IQR] VT was higher in women than in men (8.6 [7.7 to 9.6] vs. 7.6 [6.8 to 8.4] ml kg-1 PBW, P < 0.001). Compared with men, women were twice as likely not to receive LTVV [68.8 vs. 36.0%; relative risk ratio 2.1 (95% CI 1.9 to 2.1), P < 0.001]. In the mediation analysis, patients' height and actual body weight (ABW) explained 81 and 18% of the sex difference in use of LTVV, respectively; it was not explained by the use of a default VT.CONCLUSION: In this worldwide cohort of patients receiving intra-operative ventilation during general anaesthesia for surgery, women received a higher VT than men during intra-operative ventilation. The risk for a female not to receive LTVV during surgery was double that of males. Height and ABW were the two mediators of the sex difference in use of LTVV.TRIAL REGISTRATION: The study was registered at Clinicaltrials.gov, NCT01601223

New Insights into Structural and Functional Roles of Indole-3-acetic acid (IAA): Changes in DNA Topology and Gene Expression in Bacteria

Indole-3-acetic acid (IAA) is a major plant hormone that affects many cellular processes in plants, bacteria, yeast, and human cells through still unknown mechanisms. In this study, we demonstrated that the IAA-treatment of two unrelated bacteria, the Ensifer meliloti 1021 and Escherichia coli, harboring two different host range plasmids, influences the supercoiled state of the two plasmid DNAs in vivo. Results obtained from in vitro assays show that IAA interacts with DNA, leading to DNA conformational changes commonly induced by intercalating agents. We provide evidence that IAA inhibits the activity of the type IA topoisomerase, which regulates the DNA topological state in bacteria, through the relaxation of the negative supercoiled DNA. In addition, we demonstrate that the treatment of E. meliloti cells with IAA induces the expression of some genes, including the ones related to nitrogen fixation. In contrast, these genes were significantly repressed by the treatment with novobiocin, which reduces the DNA supercoiling in bacterial cells. Taking into account the overall results reported, we hypothesize that the IAA action and the DNA structure/function might be correlated and involved in the regulation of gene expression. This work points out that checking whether IAA influences the DNA topology under physiological conditions could be a useful strategy to clarify the mechanism of action of this hormone, not only in plants but also in other unrelated organisms

Nota al documento del CBV-A sulla "Conservazione dell’agrobiodiversità, sostenibilità per gli agricoltori e profili bioetici"

Il Comitato Bioetico per la Veterinaria e l’Agroalimentare ha recentemente proposto un interessante documento dal titolo "Conservazione dell’agrobiodiversità, sostenibilità per gli agricoltori e profili bioetici" il cui merito principale é di togliere l'agricoltura "dall'angolo dei cattivi" dove un certo ambientalismo l'ha da tempo relegata, per evidenziarne i non pochi meriti, sia pure senza trascurare i problemi da affrontare e da risolvere evitando inutili drammatizzazioni, ma piuttosto evidenziando le prospettive su cui puntare per renderla sempre più funzionale al bene comune della società