Univariate and multivariate Cox regression analyses of pre-operative variables for the prediction of biochemical progression after RP for 77 patients with clinically localized PCa.

<p>*Initial PSA levels were categorized as ≥10 ng/ml versus <10 ng/ml.</p>†<p>Clinical stage was categorized as T1 versus T2.</p>‡<p>Gleason score was categorized as grade 2 to 6 versus grade 7 to 10.</p>§<p>p<0.05, statistically significant.</p

Clinical and Pathologic Features of 128 Patients with PCa.

<p>Clinical and Pathologic Features of 128 Patients with PCa.</p

Kaplan-Meier estimates of PSA progression-free survival probability for the 77 patients with clinically localized PCa treated with RP, who were grouped by the baseline serum EPCA level above or below the median value of 15.20 ng/ml.

<p>Kaplan-Meier estimates of PSA progression-free survival probability for the 77 patients with clinically localized PCa treated with RP, who were grouped by the baseline serum EPCA level above or below the median value of 15.20 ng/ml.</p

Baseline serum levels of EPCA in healthy controls and PCa patients and association of EPCA levels with clinicopathological variables in 128 prostatic carcinomas.

∥<p>Categorized by the median value.</p><p>*Mann-Whitney U test.</p>†<p>Kruskal-Wallis test.</p>‡<p>Including lymph node metastases and distant metastases to bone and liver.</p>§<p>p<0.05, statistically significant.</p

Kaplan-Meier estimates of AIP-free survival probability for the 51 patients with locally advanced and metastatic PCa treated with ADT, who were grouped by the baseline serum EPCA level above or below the median value of 15.20 ng/ml.

<p>Kaplan-Meier estimates of AIP-free survival probability for the 51 patients with locally advanced and metastatic PCa treated with ADT, who were grouped by the baseline serum EPCA level above or below the median value of 15.20 ng/ml.</p

Effect of Ku80 expression on cell cycle distribution under hyperthermia.

<p>786-O-shKu80 and 786-O-scramble cells were subjected to 42°C for the indicated amount of time. Then the cell cycle distribution was measured immediately after hyperthermia. Images showing flow cytometric analysis of cell cycle distribution.</p

Ku expression was detected in 786-O cells exposed to 37°C or 42°C for the indicated amount of time.

<p><b>A</b> and <b>B:</b> Ku70 and Ku80 mRNA expression was analysed by RT-PCR. <b>C</b> and <b>D:</b> Ku70 and Ku80 protein expression was detected by Western blot. *P<0.05 compared to control.</p

Primers used in this study.

<p>Primers used in this study.</p

Hyperthermia induces apoptosis in 786-O cells.

<p>Cells were exposed to 37°C and 42°C for the indicated amount of time. Apoptotic cells were measured by flow cytometry immediately after heat treatment. <b>A:</b> Images showing flow cytometric analysis of apoptosis. <b>B:</b> The histogram shows the result from A (%). *P<0.05 compared to control. Results are representative of three independent experiments.</p

Effect of Ku80 expression on heat-sensitivity under hyperthermia.

<p>786-O-shKu80 and 786-O-scramble cells were subjected to 42°C for the indicated amount of time. Survival fractions were measured by colony formation assay after hyperthermia. <b>A:</b> Images showing colony formation assay. <b>B:</b> The histogram shows the result from A (%). *P<0.05 compared to control. Each date point is the mean of three independent experiments.</p