Histopathological findings in canine leishmaniasis ulcers.

(A) Dog 1 Ulcer with intense inflammation 4X. (B) Dog 1 Tissue granulation 20X. (C) Dog 5 Intense inflammation, plasma cells 40X. (D) Dog 26 Granuloma 20X. (E) Dog 1 Necrosis 20X. (F) Dog 2 Fibrosis (4X). (G) Dog 1 Amastigotes inside macrophage (arrow) 10X. (H) Dog 1 Amastigotes inside macrophage (arrow) 100X. (I) Dog 2 Fibrosis in blue (same of F) stained by Masson`s Trichrome 4X.</p

Cutaneous and mucosal lesions caused by <i>Leishmania (Viannia) braziliensis</i>.

The ulcerated lesions suggestive of ATL were located in the scrotal sac (A and B), muzzle (C and D) and ears (E and F), being, for the most part, single lesions, ulcerated or ulcero-crusted and of chronic evolution.</p

Frequency distribution of seven alleles in <i>L</i>. <i>braziliensis</i> locus CHR28/425451 among ATL patients and domestic dogs from Corte de Pedra, Bahia, Northeast Brazil.

Frequency distribution of seven alleles in L. braziliensis locus CHR28/425451 among ATL patients and domestic dogs from Corte de Pedra, Bahia, Northeast Brazil.</p

Clinical and demographic features of 61 dogs with ulcerated skin or mucosal lesions in the village of Corte de Pedra.

Clinical and demographic features of 61 dogs with ulcerated skin or mucosal lesions in the village of Corte de Pedra.</p

Histopathological findings in dogs with ulcerated lesions.

Histopathological findings in dogs with ulcerated lesions.</p

STARD flowchart to evaluate the diagnostic performance of IBMP chimeric antigens to detect anti-<i>Trypanosoma cruzi</i> antibodies in dog sera.

Reference Standard Tests: in-house ELISA using fractionated lysates of T. cruzi at the epimastigote and modified Gold ELISA Chagas (Rem Indústria e Comércio, Brazil).</p

Reactivity index for antigen performance for the different <i>Trypanosoma cruzi</i> strains tested.

The cut-off is set at the reactivity index value = 1.0 and the shadowed area represents the grey zone (RI = 1.0 ± 0.10). Horizontal lines and numbers for each results group represent the geometric means (± 95% CI). CI (confidence interval); Sen (sensitivity).</p

Reactivity index and diagnostic performance parameters obtained with serum samples from <i>T</i>. <i>cruzi</i>-infected and non-infected dogs.

Panel 1 (dogs experimentally infected with Y, Berenice, and Colombian T. cruzi strains); Panel 2 (dogs naturally infected with unknown T. cruzi strains). The cut-off is set at the reactivity index value = 1.0 and the shadowed area represents the grey zone (RI = 1.0 ± 0.10). Horizontal lines and numbers for each result group represent the geometric means (± 95% CI). Acc (accuracy); AUC (area under curve); Sen (sensitivity); Spe (specificity); TcP (T. cruzi-positive samples); TcN (T. cruzi-negative samples).</p

SDS-PAGE stained with Coomassie brilliant showing the chimeric purified antigens.

The T. cruzi proteins whose antigenic regions were used to construct the chimeric antigens are described on the right. Lanes 8.1 to 8.4 indicate the IBMP multi-epitope antigens (1 μg of each antigen was loaded per lane). MM: molecular weight marker.</p

Analysis of IBMP chimeric antigens cross-reactivity with sera from dogs affected by unrelated parasites infection.

The cut-off value is reactivity index = 1.0 and the shadowed area represents the grey zone (RI = 1.0 ± 0.10). ANA (anaplasmosis); BAB (babesiosis); DIR (dirofilariosis); EHR (ehrlichiosis); LEI (leishmaniasis).</p